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AB199808

Recombinant human EMAP II/AIMP1 protein

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Recombinant human EMAP II/AIMP1 protein is a Human Full Length protein, in the 147 to 312 aa range, expressed in Escherichia coli, with >98%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.

View Alternative Names

EMAP2, SCYE1, AIMP1, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, Multisynthase complex auxiliary component p43

Key facts

Purity

>98% SDS-PAGE

Purity determined by SDS-PAGE and HPLC analyses.

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, HPLC, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Determined by the apoptotic effect on MCF-7 cells using a concentration of 20-40 ng/mL.

Accession

Q12904

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water or 0.1% BSA aqueous buffer

Storage buffer

pH: 7.4 Constituents: 99% Phosphate Buffer, 0.75% Sodium chloride

storage-buffer

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Complementary Products

Primary Antibodies

AB188320

Anti-EMAP II/AIMP1 antibody [EPR14168(B)]

RabMAb|Recombinant

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] (AB188320)

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Proteins & Peptides

AB271721

Recombinant human PRMT5 + MEP50 protein (Active) (DDDDK tag N-Terminus, His tag N-Terminus)

Functional Studies - Recombinant human PRMT5 + MEP50 protein (Active) (DDDDK tag N-Terminus, His tag N-Terminus) (AB271721)

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Proteins & Peptides

AB157285

Recombinant mouse BAFF protein

Bioactive

Functional Studies - Recombinant mouse BAFF protein (AB157285)

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Proteins & Peptides

AB206448

Recombinant human Tryptophan Hydroxylase/TPH protein (Active) (His tag N-Terminus)

Functional Studies - Recombinant human Tryptophan Hydroxylase/TPH protein (Active) (His tag N-Terminus) (AB206448)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as EMAP II
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Sequence info

[{"linker":null,"sequence":"SKPIDVSRLDLRIGCIITARKHPDADSLYVEEVDVGEIAPRTVVSGLVNHVPLEQMQNRMVILLCNLKPAKMRGVLSQAMVMCASSPEKIEILAPPNGSVPGDRITFDAFPGEPDKELNPKKKIWEQIQPDLHTNDECVATYKGVPFEVKGKGVCRAQTMSNSGIK","proteinLength":"Full Length","predictedMolecularWeight":"18.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":312,"aminoAcidStart":147,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q12904","tags":[]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EMAP II also known as AIMP1 (Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1) is a protein with a mass of approximately 34 kDa. Mechanically it modulates various cellular processes including apoptosis and inflammation. This protein is expressed in several tissues with higher levels seen in the immune cells the lung and the heart. AIMP1 is part of the wider group of proteins involved in the multi-synthetase complex which ensures efficient protein synthesis.
Biological function summary

AIMP1 functions beyond its role in the multi-synthetase complex to affect cellular proliferation and differentiation. It acts as a cytokine that can influence immune responses. When secreted AIMP1 shows pro-inflammatory activity targeting endothelial cells macrophages and fibroblasts. It is important for regulating the immune system's response to stress and external stimuli thereby bridging cellular activities with immune signaling networks.

Pathways

AIMP1 integrates into both the inflammatory response pathway and apoptosis signaling. This protein interacts with TNF-alpha and IL-6 which are important players in inflammation. AIMP1's activity influences ERK and JNK pathways contributing to the regulation of apoptosis and survival of immune cells. Its relation with these pathways highlights its role as a mediator that can shift cellular balance towards survival or programmed cell death based on external cues.

AIMP1 has connections to cancer and autoimmune diseases. Studies show increased levels of AIMP1 in tumors linking it to cancer progression especially in breast cancer where it interacts with proteins like p53. Furthermore AIMP1's involvement in autoimmune disorders such as rheumatoid arthritis connects it with TNF-alpha an important inflammatory mediator. This connection suggests AIMP1's function as a potential biomarker and target for therapeutic interventions in such conditions.
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General info

Function

Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase (PubMed : 10358004). Binds tRNA. Possesses inflammatory cytokine activity (PubMed : 11306575). Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation (By similarity). Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels (By similarity). Promotes dermal fibroblast proliferation and wound repair (PubMed : 16472771). Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum (By similarity). Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelial cell apoptosis at high concentrations (PubMed : 12237313). Induces maturation of dendritic cells and monocyte cell adhesion (PubMed : 11818442). Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7 (PubMed : 19362550).

Post-translational modifications

Cleaved by caspase-7 in response to apoptosis to produce EMAP-II.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase (PubMed : 10358004). Binds tRNA. Possesses inflammatory cytokine activity (PubMed : 11306575). Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation (By similarity). Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels (By similarity). Promotes dermal fibroblast proliferation and wound repair (PubMed : 16472771). Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum (By similarity). Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelial cell apoptosis at high concentrations (PubMed : 12237313). Induces maturation of dendritic cells and monocyte cell adhesion (PubMed : 11818442). Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7 (PubMed : 19362550).
See full target information AIMP1
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