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AB130059

Recombinant Human EMG1 protein (His tag N-Terminus)

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Recombinant Human EMG1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 244 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

C2F, EMG1, Ribosomal RNA small subunit methyltransferase NEP1, 18S rRNA (pseudouridine(1248)-N1)-methyltransferase, 18S rRNA Psi1248 methyltransferase, Nucleolar protein EMG1 homolog, Protein C2f, Ribosome biogenesis protein NEP1

1 Images
SDS-PAGE - Recombinant Human EMG1 protein (His tag N-Terminus) (AB130059)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human EMG1 protein (His tag N-Terminus) (AB130059)

15% SDS-PAGE showing ab130059 (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q92979

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAAPSDGFKPRERSGGEQAQDWDALPPKRPRLGAGNKIGGRRLIVVLEGASLETVKVGKTYELLNCDKHKSILLKNGRDPGEARPDITHQSLLMLMDSPLNRAGLLQVYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRAADGPQKLLKVIKNPVSDHFPVGCMKVGTSFSIPVVSDVRELVPSSDPIVFVVGAFAHGKVSVEYTEKMVSISNYPLSAALTCAKLTTAFEEVWGVI","proteinLength":"Full Length","predictedMolecularWeight":"29.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":244,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q92979","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EMG1 protein also known as Bystin-like protein or N1-methyladenosine methyltransferase non-catalytic subunit EMG1 functions as a ribosome biogenesis factor. It weighs approximately 28 kDa. EMG1 localizes mostly in the nucleolus of cells where ribosomal assembly occurs. Its expression occurs in various tissues with significant levels observed in actively dividing cells such as those in proliferative tissues.
Biological function summary

EMG1 serves as an integral component of the small subunit processome a large ribonucleoprotein complex. Within this complex EMG1 participates in properly folding and processing precursor rRNA (pre-rRNA). This step is essential for the maturation and functionality of the small ribosomal subunit which is necessary for protein synthesis.

Pathways

The involvement of EMG1 in ribosome biogenesis places it in the pre-rRNA processing pathway. EMG1 interacts with proteins such as UTP18 and has connections to the snoRNA-guided modification system. These interactions facilitate the endonucleolytic cleavage of pre-rRNA ensuring accurate and efficient production of ribosomal subunits.

Mutations or dysregulation of EMG1 affect ribosome biogenesis possibly leading to conditions like Bowen-Conradi syndrome. This syndrome results from a specific mutation in the EMG1 gene and is marked by developmental abnormalities. Moreover disruptions in EMG1 link it indirectly to ribosomopathies disorders characterized by impaired ribosomal function.
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Specifications

Form

Liquid

Additional notes

ab130059 is purified using conventional chromatography techniques.

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General info

Function

S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position (PubMed : 20047967). Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes (By similarity). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

Sequence similarities

Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position (PubMed : 20047967). Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes (By similarity). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information EMG1
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