Recombinant Human Endonuclease V protein (GST tag N-Terminus)
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Recombinant Human Endonuclease V protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 264 aa range, expressed in Wheat germ, suitable for ELISA, WB.
View Alternative Names
Endonuclease V, hEndoV, Inosine-specific endoribonuclease, ENDOV
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human Endonuclease V protein (GST tag N-Terminus) (AB166165)
ab166165 on a 12.5% SDS-PAGE stained with Coomassie Blue.
Complementary Products
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The primary role of Endonuclease V involves maintaining genomic stability through its repair activity. This enzyme belongs to the endonuclease family which is essential for correcting mutations that arise due to deamination events. Although Endonuclease V typically acts independently it often collaborates with other proteins like DNA glycosylases to efficiently execute DNA repair mechanisms ensuring fidelity of genetic information.
Pathways
The function of Endonuclease V is highly integrated into the base excision repair (BER) pathway. This pathway is responsible for correcting small non-helix-distorting base lesions in DNA. Another key protein in this pathway is DNA polymerase beta which helps insert the correct nucleotide following excision by Endonuclease V. Additionally Endonuclease V is linked to the nucleotide excision repair (NER) pathway although its primary role remains within BER.
Specifications
Form
Liquid
General info
Function
Isoform 1. Endoribonuclease that specifically cleaves inosine-containing RNAs : cleaves RNA at the second phosphodiester bond 3' to inosine (PubMed : 23912683, PubMed : 23912718, PubMed : 25195743, PubMed : 27573237, PubMed : 31703097). Active against both single-stranded and double-stranded RNAs (PubMed : 25195743, PubMed : 31703097). Has strong preference for single-stranded RNAs (ssRNAs) toward double-stranded RNAs (dsRNAs) (PubMed : 23912718). Cleaves mRNAs and tRNAs containing inosine (PubMed : 23912683, PubMed : 31703097). Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3' (PubMed : 23912718, PubMed : 27573237). Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear : it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing (Probable). Binds branched DNA structures (PubMed : 23139746).. Isoform 6. Endoribonuclease that specifically cleaves inosine-containing RNAs : cleaves RNA at the second phosphodiester bond 3' to inosine (PubMed : 31703097). Active against both single-stranded and double-stranded RNAs (PubMed : 31703097). Cleaves tRNAs containing inosine (PubMed : 31703097).. Isoform 7. Endoribonuclease that specifically cleaves inosine-containing RNAs : cleaves RNA at the second phosphodiester bond 3' to inosine (PubMed : 31703097). Active against both single-stranded and double-stranded RNAs (PubMed : 31703097). Cleaves tRNAs containing inosine (PubMed : 31703097).
Sequence similarities
Belongs to the endonuclease V family.
Target data
Product promise
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