Recombinant human ENO1 protein is a Human Full Length protein, in the 1 to 434 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.
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- Documenta ophthalmologica. Advances in ophthalmolo 134:227-2352017Acute progressive paravascular placoid neuroretinopathy with negative-type electroretinography in paraneoplastic retinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFred K Chen et. al.PubMed 28382556
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M S I L K I H A R E I F D S R G N P T V E V D L F T S K G L F R A A V P S G A S T G I Y E A L E L R D N D K T R Y M G K G V S K A V E H I N K T I A P A L V S K K L N V T E Q E K I D K L M I E M D G T E N K S K F G A N A I L G V S L A V C K A G A V E K G V P L Y R H I A D L A G N S E V I L P V P A F N V I N G G S H A G N K L A M Q E F M I L P V G A A N F R E A M R I G A E V Y H N L K N V I K E K Y G K D A T N V G D E G G F A P N I L E N K E G L E L L K T A I G K A G Y T D K V V I G M D V A A S E F F R S G K Y D L D F K S P D D P S R Y I S P D Q L A D L Y K S F I K D Y P V V S I E D P F D Q D D W G A W Q K F T A S A G I Q V V G D D L T V T N P K R I A K A V N E K S C N C L L L K V N Q I G S V T E S L Q A C K L A Q A N G W G V M V S H R S G E T E D T F I A D L V V G L C T G Q I K T G A P C R S E R L A K Y N Q L L R I E E E L G S K A K F A G R N F R N P L A K
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate (PubMed:1369209, PubMed:29775581). In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed:10802057, PubMed:12666133, PubMed:2005901, PubMed:29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed:12666133). Stimulates immunoglobulin production (PubMed:1369209). Isoform MBP-1. Binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
Alternative names
ENO1L1, MBPB1, MPB1, ENO1, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, NNE
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Recombinant human ENO1 protein is a Human Full Length protein, in the 1 to 434 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity: > 13 units/ml. One unit will convert 1.0 μmole of 2-phosphoglycerate to phosphoenol pyruvate per minute at pH 7.5 at 25°C.
- Accession
- P06733-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.012% Magnesium sulphate
Sequence info
Amino acid sequence
- M S I L K I H A R E I F D S R G N P T V E V D L F T S K G L F R A A V P S G A S T G I Y E A L E L R D N D K T R Y M G K G V S K A V E H I N K T I A P A L V S K K L N V T E Q E K I D K L M I E M D G T E N K S K F G A N A I L G V S L A V C K A G A V E K G V P L Y R H I A D L A G N S E V I L P V P A F N V I N G G S H A G N K L A M Q E F M I L P V G A A N F R E A M R I G A E V Y H N L K N V I K E K Y G K D A T N V G D E G G F A P N I L E N K E G L E L L K T A I G K A G Y T D K V V I G M D V A A S E F F R S G K Y D L D F K S P D D P S R Y I S P D Q L A D L Y K S F I K D Y P V V S I E D P F D Q D D W G A W Q K F T A S A G I Q V V G D D L T V T N P K R I A K A V N E K S C N C L L L K V N Q I G S V T E S L Q A C K L A Q A N G W G V M V S H R S G E T E D T F I A D L V V G L C T G Q I K T G A P C R S E R L A K Y N Q L L R I E E E L G S K A K F A G R N F R N P L A K
- Accession
- P06733
- Protein length
- Full Length
- Predicted molecular weight
- 47.1 kDa
- Amino acids
- 1 to 434
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate (PubMed:1369209, PubMed:29775581). In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed:10802057, PubMed:12666133, PubMed:2005901, PubMed:29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed:12666133). Stimulates immunoglobulin production (PubMed:1369209).
- Sequence similarities
Belongs to the enolase family.
- Post-translational modifications
ISGylated.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product is manufactured by BioVision, an Abcam company and was previously called 6363 Human Recombinant Alpha-Enolase. 6363-100 is the same size as the 100 μg size of ab167976.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Enolase 1 or alpha-enolase is an important enzyme in the glycolytic pathway. It catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. We also refer to it as an enolase reaction. ENO1 protein has a molecular mass of approximately 47 kDa and is expressed in most tissues but found most abundantly in the brain muscle and liver. Additionally ENO1 can exist in both cytosolic and plasma membrane-associated forms playing functional roles in different cellular contexts.
- Biological function summary
The ENO1 protein serves multiple functions beyond its glycolytic activity. It also acts as a plasminogen receptor on the cell surface aiding in the modulation of pericellular and extracellular matrix degradation processes. Through this role ENO1 influences cell migration and tissue remodeling. This protein can form part of larger protein complexes contributing to its versatility in various cellular functions.
- Pathways
ENO1 plays a critical role in glycolysis a central metabolic pathway that converts glucose into pyruvate generating ATP as a result. In this pathway ENO1 closely interacts with other enzymes like phosphoglycerate kinase and pyruvate kinase. Additionally as a plasminogen receptor ENO1 links to the plasminogen activation pathway affecting fibrinolysis and cellular response to tissue injury.
- Associated diseases and disorders
ENO1 is associated with cancer and neurodegenerative diseases. Overexpression or aberrant regulation of ENO1 often correlates with tumor progression and metastasis in cancers such as lung and breast cancer. In neurodegenerative disorders like Alzheimer's disease abnormal functioning of ENO1 can impact brain energy metabolism and neuron degeneration. ENO1's interaction with other proteins such as beta-enolase further connects it to these conditions influencing disease mechanisms and patient outcomes.
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SDS-PAGE - Recombinant human ENO1 protein (ab167976)
15% SDS PAGE of ab167976 (3μg).
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