AB89248

Recombinant human ENO1 protein (Active) (Tag Free)

Bioactive
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(4 Publications)

Recombinant human ENO1 protein (Active) (Tag Free) is a Human Full Length protein, in the 1 to 434 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

ENO1L1, MBPB1, MPB1, ENO1, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, NNE

2 Images

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Western blot - Recombinant human ENO1 protein (Active) (Tag Free) (AB89248)

All lanes:

Anti-ENO1 antibody (ab85086) at 1/1000 dilution

All lanes:

Western blot - Recombinant human ENO1 protein (Active) (Tag Free) (ab89248) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 30s

SDS-PAGE - Recombinant human ENO1 protein (Active) (AB89248)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human ENO1 protein (Active) (AB89248)

SDS-PAGE of ab89248 (3µg) under reducing condition and visualized by coomassie blue stain.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Applications

SDS-PAGE, WB, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity is > 20,000pmol/min/ug.

Obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.

Accession

P06733

Animal free

Carrier free

Species

Human

Storage buffer

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.012% Magnesium sulphate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab89248 can be used as a WB positive control in conjunction with ab85086." }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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Product details

MW confirmed by MALDI-TOF.

Endotoxin level determined by LAL method.

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Sequence info

[{"sequence":"MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK","proteinLength":"Full Length","predictedMolecularWeight":"47.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":434,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P06733","tags":[]}]

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

True

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Enolase 1 or alpha-enolase is an important enzyme in the glycolytic pathway. It catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. We also refer to it as an enolase reaction. ENO1 protein has a molecular mass of approximately 47 kDa and is expressed in most tissues but found most abundantly in the brain muscle and liver. Additionally ENO1 can exist in both cytosolic and plasma membrane-associated forms playing functional roles in different cellular contexts.

Biological function summary

The ENO1 protein serves multiple functions beyond its glycolytic activity. It also acts as a plasminogen receptor on the cell surface aiding in the modulation of pericellular and extracellular matrix degradation processes. Through this role ENO1 influences cell migration and tissue remodeling. This protein can form part of larger protein complexes contributing to its versatility in various cellular functions.

Pathways

ENO1 plays a critical role in glycolysis a central metabolic pathway that converts glucose into pyruvate generating ATP as a result. In this pathway ENO1 closely interacts with other enzymes like phosphoglycerate kinase and pyruvate kinase. Additionally as a plasminogen receptor ENO1 links to the plasminogen activation pathway affecting fibrinolysis and cellular response to tissue injury.

ENO1 is associated with cancer and neurodegenerative diseases. Overexpression or aberrant regulation of ENO1 often correlates with tumor progression and metastasis in cancers such as lung and breast cancer. In neurodegenerative disorders like Alzheimer's disease abnormal functioning of ENO1 can impact brain energy metabolism and neuron degeneration. ENO1's interaction with other proteins such as beta-enolase further connects it to these conditions influencing disease mechanisms and patient outcomes.

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Specifications

Form

Liquid

Additional notes

ab89248 is purified using conventional chromatography techniques.

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General info

Function

Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate (PubMed : 1369209, PubMed : 29775581). In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed : 10802057, PubMed : 12666133, PubMed : 2005901, PubMed : 29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed : 12666133). Stimulates immunoglobulin production (PubMed : 1369209).. Isoform MBP-1. Binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

Sequence similarities

Belongs to the enolase family.

Post-translational modifications

ISGylated.. Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity.

Subcellular localisation

Nucleus

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information ENO1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 14:74 PubMed38849850

2024

Tumour-specific phosphorylation of serine 419 drives alpha-enolase (ENO1) nuclear export in triple negative breast cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Morgan L Marshall,Kim Yc Fung,David A Jans,Kylie M Wagstaff

Stem cell research & therapy 14:63 PubMed37013624

2023

Crotonylation of GAPDH regulates human embryonic stem cell endodermal lineage differentiation and metabolic switch.

Applications

Unspecified application

Species

Unspecified reactive species

Jingran Zhang,Guang Shi,Junjie Pang,Xing Zhu,Qingcai Feng,Jie Na,Wenbin Ma,Dan Liu,Zhou Songyang

International journal of molecular sciences 24: PubMed36614179

2023

ENO1 Promotes OSCC Migration and Invasion by Orchestrating IL-6 Secretion from Macrophages via a Positive Feedback Loop.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Lin,Wenwen Zhang,Luyao Liu,Weibo Li,Yafei Li,Bo Li

PloS one 9:e88314 PubMed25171719

2014

Modulation of α-enolase post-translational modifications by dengue virus: increased secretion of the basic isoforms in infected hepatic cells.

Applications

ELISA

Species

Unspecified reactive species

Luiza M Higa,Bruno M Curi,Renato S Aguiar,Cynthia C Cardoso,André G De Lorenzi,Silvia L F Sena,Russolina B Zingali,Andrea T Da Poian

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Recombinant human ENO1 protein (Active) (Tag Free)

Western blot - Recombinant human ENO1 protein (Active) (Tag Free) (AB89248)

SDS-PAGE - Recombinant human ENO1 protein (Active) (AB89248)

Key facts

Purity

Endotoxin level

Expression system

Tags

Applications

Biologically active

Biological activity

Accession

Animal free

Carrier free

Species

Storage buffer

Reactivity data

Product details

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Specifications

Form

Additional notes

General info

Function

Sequence similarities

Post-translational modifications

Subcellular localisation

Product protocols

Target data

Publications (4)

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

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