Recombinant human ENO3 protein is a Human Full Length protein, in the 1 to 434 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- Yes
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A M Q K I F A R E I L D S R G N P T V E V D L H T A K G R F R A A V P S G A S T G I Y E A L E L R D G D K G R Y L G K G V L K A V E N I N S T L G P A L L Q K K L S V A D Q E K V D K F M I E L D G T E N K S K F G A N A I L G V S L A V C K A G A A E K G V P L Y R H I A D L A G N P D L I L P V P A F N V I N G G S H A G N K L A M Q E F M I L P V G A S S F K E A M R I G A E V Y H H L K G V I K A K Y G K D A T N V G D E G G F A P N I L E N N E A L E L L K T A I Q A A G Y P D K V V I G M D V A A S E F Y R N G K Y D L D F K S P D D P A R H I T G E K L G E L Y K S F I K N Y P V V S I E D P F D Q D D W A T W T S F L S G V N I Q I V G D D L T V T N P K R I A Q A V E K K A C N C L L L K V N Q I G S V T E S I Q A C K L A Q S N G W G V M V S H R S G E T E D T F I A D L V V G L C T G Q I K T G A P C R S E R L A K Y N Q L M R I E E A L G D K A I F A G R K F R N P K A K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Appears to have a function in striated muscle development and regeneration.
Alternative names
Beta-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, Skeletal muscle enolase, MSE, ENO3
Recommended products
Recombinant human ENO3 protein is a Human Full Length protein, in the 1 to 434 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- Yes
- Biological activity
- Specific activity is > 5,000pmol/min/μg, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37°C.
- Accession
- P13929-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A M Q K I F A R E I L D S R G N P T V E V D L H T A K G R F R A A V P S G A S T G I Y E A L E L R D G D K G R Y L G K G V L K A V E N I N S T L G P A L L Q K K L S V A D Q E K V D K F M I E L D G T E N K S K F G A N A I L G V S L A V C K A G A A E K G V P L Y R H I A D L A G N P D L I L P V P A F N V I N G G S H A G N K L A M Q E F M I L P V G A S S F K E A M R I G A E V Y H H L K G V I K A K Y G K D A T N V G D E G G F A P N I L E N N E A L E L L K T A I Q A A G Y P D K V V I G M D V A A S E F Y R N G K Y D L D F K S P D D P A R H I T G E K L G E L Y K S F I K N Y P V V S I E D P F D Q D D W A T W T S F L S G V N I Q I V G D D L T V T N P K R I A Q A V E K K A C N C L L L K V N Q I G S V T E S I Q A C K L A Q S N G W G V M V S H R S G E T E D T F I A D L V V G L C T G Q I K T G A P C R S E R L A K Y N Q L M R I E E A L G D K A I F A G R K F R N P K A K
- Accession
- P13929
- Protein length
- Full Length
- Predicted molecular weight
- 49 kDa
- Amino acids
- 1 to 434
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab113127 was purified using conventional chromatography.
General info
- Function
Glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Appears to have a function in striated muscle development and regeneration.
- Sequence similarities
Belongs to the enolase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ENO3 protein also known as beta-enolase mechanically functions as an enzyme involved in glycolysis catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate. This protein has a molecular mass of approximately 47 kDa. It is specifically expressed in skeletal muscle tissue where it plays a critical role in energy metabolism. Beta-enolase is an isoform of enolase that is distinct from the alpha (ENO1) and gamma (ENO2) isoforms which have different tissue specificities.
- Biological function summary
Beta-enolase supports cellular energy production in muscles. It does not typically form part of a protein complex but acts independently in the glycolytic pathway. Its function in glycolysis is important for providing ATP to muscle cells especially during periods of high energy demand such as exercise. The efficient conversion of metabolic substrates in this pathway is essential for muscle function and maintenance.
- Pathways
Beta-enolase operates within the glycolytic pathway and plays an important role in energy production. This pathway is fundamental for breaking down glucose to provide ATP the energy currency of the cell. Beta-enolase works closely with other key glycolytic enzymes such as hexokinase and phosphofructokinase. Its role is indispensable in maintaining the flow of carbon through glycolysis ensuring robust energy supply for muscle contraction.
- Associated diseases and disorders
Beta-enolase is associated with diseases like glycogen storage disease type XIII and muscle-related disorders. Mutations in ENO3 gene can lead to reduced energetic efficiency and impact muscular health. Of particular note is the connection with related glycolytic proteins which may exhibit compensatory or contributing roles in these disorders. This highlights the importance of beta-enolase and its interactions in maintaining muscle physiology and energy homeostasis.
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1 product image
SDS-PAGE - Recombinant human ENO3 protein (ab113127)
15% SDS-PAGE analysis of ENO3 protein (ab113127; 3 μg).
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