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AB113127

Recombinant human ENO3 protein

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Recombinant human ENO3 protein is a Human Full Length protein, in the 1 to 434 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec, FuncS.

View Alternative Names

Beta-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, Skeletal muscle enolase, MSE, ENO3

1 Images
SDS-PAGE - Recombinant human ENO3 protein (AB113127)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human ENO3 protein (AB113127)

15% SDS-PAGE analysis of ENO3 protein (ab113127; 3 μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE, Mass Spec

applications

Biologically active

Yes

Biological activity

Specific activity is > 5,000pmol/min/μg, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37°C.

Accession

P13929

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAMQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGKGVLKAVENINSTLGPALLQKKLSVADQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPKAK","proteinLength":"Full Length","predictedMolecularWeight":"49 kDa","actualMolecularWeight":null,"aminoAcidEnd":434,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P13929","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ENO3 protein also known as beta-enolase mechanically functions as an enzyme involved in glycolysis catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate. This protein has a molecular mass of approximately 47 kDa. It is specifically expressed in skeletal muscle tissue where it plays a critical role in energy metabolism. Beta-enolase is an isoform of enolase that is distinct from the alpha (ENO1) and gamma (ENO2) isoforms which have different tissue specificities.
Biological function summary

Beta-enolase supports cellular energy production in muscles. It does not typically form part of a protein complex but acts independently in the glycolytic pathway. Its function in glycolysis is important for providing ATP to muscle cells especially during periods of high energy demand such as exercise. The efficient conversion of metabolic substrates in this pathway is essential for muscle function and maintenance.

Pathways

Beta-enolase operates within the glycolytic pathway and plays an important role in energy production. This pathway is fundamental for breaking down glucose to provide ATP the energy currency of the cell. Beta-enolase works closely with other key glycolytic enzymes such as hexokinase and phosphofructokinase. Its role is indispensable in maintaining the flow of carbon through glycolysis ensuring robust energy supply for muscle contraction.

Beta-enolase is associated with diseases like glycogen storage disease type XIII and muscle-related disorders. Mutations in ENO3 gene can lead to reduced energetic efficiency and impact muscular health. Of particular note is the connection with related glycolytic proteins which may exhibit compensatory or contributing roles in these disorders. This highlights the importance of beta-enolase and its interactions in maintaining muscle physiology and energy homeostasis.
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Specifications

Form

Liquid

Additional notes

ab113127 was purified using conventional chromatography.

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General info

Function

Glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Appears to have a function in striated muscle development and regeneration.

Sequence similarities

Belongs to the enolase family.

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Product protocols

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Target data

Glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Appears to have a function in striated muscle development and regeneration.
See full target information ENO3
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Complementary Products

Primary Antibodies

AB157474

Anti-ENO1 + ENO3 antibody [EPR11366(B)]

RabMAb|Recombinant

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (AB157474)

  • 0
  • 0
Primary Antibodies

AB223287

Anti-FSTL1/FRP antibody [EPR21933-155]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-FSTL1/FRP antibody [EPR21933-155] (AB223287)

  • 0
  • 0
Primary Antibodies

AB30682

Anti-SREBP2 antibody

Western blot - Anti-SREBP2 antibody (AB30682)

  • 4
  • 11
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15

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