Recombinant Human ENPP1/PC1 protein is a Human Fragment protein, in the 98 to 925 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, WB.
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- BioMed research international 2019:52148212019Misleading Westerns: Common Quantification Mistakes in Western Blot Densitometry and Proposed Corrective Measures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTrent A J Butler et. al.PubMed 30800670
Key facts
- Purity
>95% Densitometry
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
Amino acid sequence
A S K P S C A K E V K S C K G R C F E R T F G N C R C D A A C V E L G N C C L D Y Q E T C I E P E H I W T C N K F R C G E K R L T R S L C A C S D D C K D K G D C C I N Y S S V C Q G E K S W V E E P C E S I N E P Q C P A G F E T P P T L L F S L D G F R A E Y L H T W G G L L P V I S K L K K C G T Y T K N M R P V Y P T K T F P N H Y S I V T G L Y P E S H G I I D N K M Y D P K M N A S F S L K S K E K F N P E W Y K G E P I W V T A K Y Q G L K S G T F F W P G S D V E I N G I F P D I Y K M Y N G S V P F E E R I L A V L Q W L Q L P K D E R P H F Y T L Y L E E P D S S G H S Y G P V S S E V I K A L Q R V D G M V G M L M D G L K E L N L H R C L N L I L I S D H G M E Q G S C K K Y I Y L N K Y L G D V K N I K V I Y G P A A R L R P S D V P D K Y Y S F N Y E G I A R N L S C R E P N Q H F K P Y L K H F L P K R L H F A K S D R I E P L T F Y L D P Q W Q L A L N P S E R K Y C G S G F H G S D N V F S N M Q A L F V G Y G P G F K H G I E A D T F E N I E V Y N L M C D L L N L T P A P N N G T H G S L N H L L K N P V Y T P K H P K E V H P L V Q C P F T R N P R D N L G C S C N P S I L P I E D F Q T Q F N L T V A E E K I I K H E T L P Y G R P R V L Q K E N T I C L L S Q H Q F M S G Y S Q D I L M P L W T S Y T V D R N D S F S T E D F S N C L Y Q D F R I P L S P V H K C S F Y K N N T K V S Y G F L S P P Q L N K N S S G I Y S E A L L T T N I V P M Y Q S F Q V I W R Y F H D T L L R K Y A E E R N G V N V V S G P V F D F D Y D G R C D S L E N L R Q K R R V I R N Q E I L I P T H F F I V L T S C K D T S Q T P L H C E N L D T L A F I L P H R T D N S E S C V H G K H D S S W V E E L L M L H R A R I T D V E H I T G L S F Y Q Q R K E P V S D I L K L K T H L P T F S Q E D G P K L H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Nucleotide pyrophosphatase that generates diphosphate (PPi) and functions in bone mineralization and soft tissue calcification by regulating pyrophosphate levels (By similarity). PPi inhibits bone mineralization and soft tissue calcification by binding to nascent hydroxyapatite crystals, thereby preventing further growth of these crystals (PubMed:11004006). Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP (PubMed:27467858, PubMed:8001561, PubMed:25344812). May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling (PubMed:27467858, PubMed:8001561). Inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling; it is however unclear whether hedgehog inhibition is direct or indirect (By similarity). Appears to modulate insulin sensitivity and function (PubMed:10615944). Also involved in melanogenesis (PubMed:28964717). Also able to hydrolyze 2'-3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production (PubMed:25344812). 2'-3'-cGAMP degradation takes place in the lumen or extracellular space, and not in the cytosol where it is produced; the role of 2'-3'-cGAMP hydrolysis is therefore unclear (PubMed:25344812). Not able to hydrolyze the 2'-3'-cGAMP linkage isomer 3'-3'-cGAMP (PubMed:25344812).
Alternative names
Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, E-NPP 1, Membrane component chromosome 6 surface marker 1, Phosphodiesterase I/nucleotide pyrophosphatase 1, Plasma-cell membrane glycoprotein PC-1, M6S1, PDNP1, PC1, NPPS, ENPP1
Recommended products
Recombinant Human ENPP1/PC1 protein is a Human Fragment protein, in the 98 to 925 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Purity
>95% Densitometry
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
- Accession
- P22413-1
- Animal free
No
- Species
Human
- Reconstitution
Add 200µl deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
- Concentration
- Storage buffer
Constituents: 99% Phosphate Buffer, 0.43% Sodium chloride
Sequence info
Amino acid sequence
A S K P S C A K E V K S C K G R C F E R T F G N C R C D A A C V E L G N C C L D Y Q E T C I E P E H I W T C N K F R C G E K R L T R S L C A C S D D C K D K G D C C I N Y S S V C Q G E K S W V E E P C E S I N E P Q C P A G F E T P P T L L F S L D G F R A E Y L H T W G G L L P V I S K L K K C G T Y T K N M R P V Y P T K T F P N H Y S I V T G L Y P E S H G I I D N K M Y D P K M N A S F S L K S K E K F N P E W Y K G E P I W V T A K Y Q G L K S G T F F W P G S D V E I N G I F P D I Y K M Y N G S V P F E E R I L A V L Q W L Q L P K D E R P H F Y T L Y L E E P D S S G H S Y G P V S S E V I K A L Q R V D G M V G M L M D G L K E L N L H R C L N L I L I S D H G M E Q G S C K K Y I Y L N K Y L G D V K N I K V I Y G P A A R L R P S D V P D K Y Y S F N Y E G I A R N L S C R E P N Q H F K P Y L K H F L P K R L H F A K S D R I E P L T F Y L D P Q W Q L A L N P S E R K Y C G S G F H G S D N V F S N M Q A L F V G Y G P G F K H G I E A D T F E N I E V Y N L M C D L L N L T P A P N N G T H G S L N H L L K N P V Y T P K H P K E V H P L V Q C P F T R N P R D N L G C S C N P S I L P I E D F Q T Q F N L T V A E E K I I K H E T L P Y G R P R V L Q K E N T I C L L S Q H Q F M S G Y S Q D I L M P L W T S Y T V D R N D S F S T E D F S N C L Y Q D F R I P L S P V H K C S F Y K N N T K V S Y G F L S P P Q L N K N S S G I Y S E A L L T T N I V P M Y Q S F Q V I W R Y F H D T L L R K Y A E E R N G V N V V S G P V F D F D Y D G R C D S L E N L R Q K R R V I R N Q E I L I P T H F F I V L T S C K D T S Q T P L H C E N L D T L A F I L P H R T D N S E S C V H G K H D S S W V E E L L M L H R A R I T D V E H I T G L S F Y Q Q R K E P V S D I L K L K T H L P T F S Q E D G P K L H H H H H H
- Accession
- Protein length
Fragment
- Predicted molecular weight
96.5 kDa
- Amino acids
98 to 925
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
- Additional notes
ab167943 was purifed using Ni-NTA chromatography.
General info
- Function
Nucleotide pyrophosphatase that generates diphosphate (PPi) and functions in bone mineralization and soft tissue calcification by regulating pyrophosphate levels (By similarity). PPi inhibits bone mineralization and soft tissue calcification by binding to nascent hydroxyapatite crystals, thereby preventing further growth of these crystals (PubMed:11004006). Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP (PubMed:27467858, PubMed:8001561, PubMed:25344812). May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling (PubMed:27467858, PubMed:8001561). Inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling; it is however unclear whether hedgehog inhibition is direct or indirect (By similarity). Appears to modulate insulin sensitivity and function (PubMed:10615944). Also involved in melanogenesis (PubMed:28964717). Also able to hydrolyze 2'-3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production (PubMed:25344812). 2'-3'-cGAMP degradation takes place in the lumen or extracellular space, and not in the cytosol where it is produced; the role of 2'-3'-cGAMP hydrolysis is therefore unclear (PubMed:25344812). Not able to hydrolyze the 2'-3'-cGAMP linkage isomer 3'-3'-cGAMP (PubMed:25344812).
- Sequence similarities
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
- Post-translational modifications
Autophosphorylated as part of the catalytic cycle of phosphodiesterase/pyrophosphatase activity.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-80°C
Notes
This product was previously labelled as ENPP1
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ENPP1 also known as PC-1 is a type II transmembrane glycoprotein with a molecular weight of approximately 130 kDa. It is expressed in various tissues including the liver kidney and bone. ENPP1 exhibits the enzymatic activity of nucleotide pyrophosphatase/phosphodiesterase which hydrolyzes ATP to AMP and diphosphate. This activity is important for the regulation of extracellular nucleotide levels and mineralization processes. Researchers often utilize the ENPP1 assay to study its function and activity.
- Biological function summary
ENPP1 significantly influences bone mineralization and soft tissue calcification. It interacts with proteins like ectonucleoside triphosphate diphosphohydrolase-1 (ENTPD1) impacting cellular uptake of calcified materials. The protein is also involved in the regulatory networks responsible for controlling insulin signaling impacting metabolic processes. ENPP1 may form complexes with other proteins which can finely tune its biological activity and effects.
- Pathways
ENPP1 plays a role in the insulin signaling pathway and the pyrophosphate pathway. Via its participation in insulin signaling ENPP1 regulates glucose metabolism and influences insulin sensitivity. It affects pathways related to mineral metabolism where it interacts with proteins like ENPP3. By regulating extracellular pyrophosphate levels ENPP1 contributes to inorganic phosphate homeostasis and influences skeletal development and maintenance.
- Associated diseases and disorders
ENPP1 has associations with type 2 diabetes and generalized arterial calcification of infancy (GACI). Its role in type 2 diabetes relates to its ability to inhibit insulin receptor function therefore contributing to insulin resistance. ENPP1 also connects to proteins involved in vascular calcification such as matrix Gla protein (MGP) in the context of GACI. These associations highlight the importance of ENPP1 in metabolic and calcification disorders.
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1 product image
SDS-PAGE - Recombinant Human ENPP1/PC1 protein (ab167943)
10% SDS-PAGE analysis of ab167943.
Lane 1: reduced and boiled sample, 2.5μg/lane.
Lane 2: non-reduced and non-boiled sample, 2.5μg/lane.
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