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AB92932

Recombinant Human ENSA protein

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(2 Publications)

Recombinant Human ENSA protein is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

Alpha-endosulfine, ARPP-19e, ENSA

1 Images
SDS-PAGE - Recombinant Human ENSA protein (AB92932)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ENSA protein (AB92932)

ab92932 staining ENSA protein on a 15% SDS-PAGE (3 μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O43768

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSQKQEEENPAEETGEEKQDTQEKEGILPERAEEAKLKAKYPSLGQKPGGSDFLMKRLQKGQKYFDSGDYNMAKAKMKNKQLPSAGPDKNLVTGDHIPTPQDLPQRKSSLVTSKLAGGQVE","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O43768","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENSA also known as alpha-endosulfine is a protein with a mass of approximately 13 kDa. ENSA is part of the endosulfine family and finds expression in various tissues including the brain and pancreas. This protein primarily acts as an endogenous ligand for sulfonylurea receptor and has a role in regulating potassium channels. Its structural and functional characteristics highlight its importance in cellular activities.
Biological function summary

ENSA functions as a modulator of protein phosphatase 2A (PP2A) a critical enzyme in controlling cell growth and division. ENSA by inhibiting the catalytic activity of PP2A influences key regulatory mechanisms in cells. It also interacts with ARPP-19 a protein that enhances its inhibitory action contributing to the regulation of cellular processes such as mitosis and signal transduction.

Pathways

ENSA plays a significant role in the mTOR signaling pathway a major regulator of cell growth metabolism and survival. In this pathway ENSA interacts with various proteins including the mTOR itself to modulate the phosphorylation state of downstream targets affecting cellular responses to nutrient availability. Additionally ENSA is involved in the regulation of glucose metabolism through its interaction in the AMPK signaling pathway contributing to energy homeostasis in cells.

ENSA demonstrates a connection to metabolic disorders such as type 2 diabetes by modulating insulin secretion and action. ENSA impacts the glucose regulatory processes through its association with proteins like the sulfonylurea receptor important in insulin release from pancreatic beta cells. Moreover ENSA's involvement in the dysregulation of cell growth pathways relates to certain cancers indicating its potential role in tumorigenesis through its interaction with PP2A and associated signaling components.
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Specifications

Form

Liquid

Additional notes

ab92932 is purified using conventional chromatography techniques.

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General info

Function

Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.

Sequence similarities

Belongs to the endosulfine family.

Post-translational modifications

Phosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A (By similarity). Phosphorylated by PKA.

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Product protocols

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Target data

Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.
See full target information Alpha-endosulfine
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:10568 PubMed35732702

2022

SILAC kinase screen identifies potential MASTL substrates.

Applications

Unspecified application

Species

Unspecified reactive species

Kamila A Marzec,Samuel Rogers,Rachael McCloy,Benjamin L Parker,David E James,D Neil Watkins,Andrew Burgess

Molecular psychiatry 27:1816-1828 PubMed34737456

2021

Somatostatin-evoked Aβ catabolism in the brain: Mechanistic involvement of α-endosulfine-K channel pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Naoto Watamura,Naomasa Kakiya,Per Nilsson,Satoshi Tsubuki,Naoko Kamano,Mika Takahashi,Shoko Hashimoto,Hiroki Sasaguri,Takashi Saito,Takaomi C Saido
View all publications
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