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Recombinant Human ENSA protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human ENSA protein (AB92932), expandable thumbnail

Publications

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M S Q K Q E E E N P A E E T G E E K Q D T Q E K E G I L P E R A E E A K L K A K Y P S L G Q K P G G S D F L M K R L Q K G Q K Y F D S G D Y N M A K A K M K N K Q L P S A G P D K N L V T G D H I P T P Q D L P Q R K S S L V T S K L A G G Q V E

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.

Alternative names

Recommended products

Recombinant Human ENSA protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE

Accession
O43768-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Sequence info

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M S Q K Q E E E N P A E E T G E E K Q D T Q E K E G I L P E R A E E A K L K A K Y P S L G Q K P G G S D F L M K R L Q K G Q K Y F D S G D Y N M A K A K M K N K Q L P S A G P D K N L V T G D H I P T P Q D L P Q R K S S L V T S K L A G G Q V E

Accession

O43768

Protein length

Full Length

Nature

Recombinant

Specifications

Form

Liquid

Additional notes

ab92932 is purified using conventional chromatography techniques.

General info

Function

Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.

Sequence similarities

Belongs to the endosulfine family.

Post-translational modifications

Phosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A (By similarity). Phosphorylated by PKA.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ENSA also known as alpha-endosulfine is a protein with a mass of approximately 13 kDa. ENSA is part of the endosulfine family and finds expression in various tissues including the brain and pancreas. This protein primarily acts as an endogenous ligand for sulfonylurea receptor and has a role in regulating potassium channels. Its structural and functional characteristics highlight its importance in cellular activities.

Biological function summary

ENSA functions as a modulator of protein phosphatase 2A (PP2A) a critical enzyme in controlling cell growth and division. ENSA by inhibiting the catalytic activity of PP2A influences key regulatory mechanisms in cells. It also interacts with ARPP-19 a protein that enhances its inhibitory action contributing to the regulation of cellular processes such as mitosis and signal transduction.

Pathways

ENSA plays a significant role in the mTOR signaling pathway a major regulator of cell growth metabolism and survival. In this pathway ENSA interacts with various proteins including the mTOR itself to modulate the phosphorylation state of downstream targets affecting cellular responses to nutrient availability. Additionally ENSA is involved in the regulation of glucose metabolism through its interaction in the AMPK signaling pathway contributing to energy homeostasis in cells.

Associated diseases and disorders

ENSA demonstrates a connection to metabolic disorders such as type 2 diabetes by modulating insulin secretion and action. ENSA impacts the glucose regulatory processes through its association with proteins like the sulfonylurea receptor important in insulin release from pancreatic beta cells. Moreover ENSA's involvement in the dysregulation of cell growth pathways relates to certain cancers indicating its potential role in tumorigenesis through its interaction with PP2A and associated signaling components.

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1 product image

  • SDS-PAGE - Recombinant Human ENSA protein (ab92932), expandable thumbnail

    SDS-PAGE - Recombinant Human ENSA protein (ab92932)

    ab92932 staining ENSA protein on a 15% SDS-PAGE (3 μg)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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