Recombinant Human Entactin/NID protein is a Human Fragment protein, in the 1148 to 1247 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
E G L Q Y P F A V T S Y G K N L Y F T D W K M N S V V A L D L A I S K E T D A F Q P H K Q T R L Y G I T T A L S Q C P Q G H N Y C S V N N G G C T H L C L A T P G S R T C R C P D N T L G V D C I E R K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
Alternative names
NID, NID1, Nidogen-1, NID-1, Entactin
Recommended products
Recombinant Human Entactin/NID protein is a Human Fragment protein, in the 1148 to 1247 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P14543-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- E G L Q Y P F A V T S Y G K N L Y F T D W K M N S V V A L D L A I S K E T D A F Q P H K Q T R L Y G I T T A L S Q C P Q G H N Y C S V N N G G C T H L C L A T P G S R T C R C P D N T L G V D C I E R K
- Accession
- P14543
- Protein length
- Fragment
- Amino acids
- 1148 to 1247
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
- Post-translational modifications
N- and O-glycosylated.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Entactin.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Entactin also known as nidogen-1 or NID1 is a significant glycoprotein of the extracellular matrix with a molecular mass of approximately 150 kDa. This protein is often referred to as nidogen and nidogen 1. It localizes mainly in basement membranes across various tissues. Entactin serves as an important structural component and mediator between other extracellular molecules contributing significantly to the maintenance and integrity of the basement membrane structure.
- Biological function summary
Entactin interacts closely with laminin and type IV collagen within the extracellular matrix forming an important part of the basement membrane complex. This protein aids in the stabilization and linkage of these matrices playing an essential role in cellular adhesion and migration processes. By facilitating the structural assembly of basement membranes entactin supports cellular signaling pathways that are involved in tissue development and repair.
- Pathways
Entactin acts within the integrin signaling pathway and the basement membrane formation pathway. It interacts with integrin receptors on the cell surface thereby influencing processes like cell adhesion and morphogenesis. Through these pathways entactin signals in coordination with laminins and collagens building a robust structural framework necessary for tissue integrity and function.
- Associated diseases and disorders
Entactin's disruption has links to cancer and certain genetic disorders affecting basement membrane integrity. Aberrant expression or dysfunction of entactin can contribute to the progression of tumor invasion due to its pivotal role in cellular adhesion. Furthermore it associates with muscular dystrophies through interactions with laminins and collagens highlighting its importance in maintaining normal tissue architecture and function.
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1 product image
SDS-PAGE - Recombinant Human Entactin/NID protein (ab158997)
ab158997 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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