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Recombinant Human Entactin/NID protein is a Human Fragment protein, in the 1148 to 1247 aa range, expressed in Wheat germ and suitable for ELISA, WB.

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Images

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

Biologically active

No

Amino acid sequence

E G L Q Y P F A V T S Y G K N L Y F T D W K M N S V V A L D L A I S K E T D A F Q P H K Q T R L Y G I T T A L S Q C P Q G H N Y C S V N N G G C T H L C L A T P G S R T C R C P D N T L G V D C I E R K

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Reactivity data

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

Alternative names

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Recombinant Human Entactin/NID protein is a Human Fragment protein, in the 1148 to 1247 aa range, expressed in Wheat germ and suitable for ELISA, WB.

Alternative names

Key facts

Expression system

Wheat germ

Applications

ELISA, WB

Accession
P14543-1
Animal free

No

Species

Human

Concentration
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Storage buffer

pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione

Sequence info

Amino acid sequence

E G L Q Y P F A V T S Y G K N L Y F T D W K M N S V V A L D L A I S K E T D A F Q P H K Q T R L Y G I T T A L S Q C P Q G H N Y C S V N N G G C T H L C L A T P G S R T C R C P D N T L G V D C I E R K

Accession

P14543

Protein length

Fragment

Amino acids

1148 to 1247

Nature

Recombinant

Tags

GST tag N-Terminus

Specifications

Form

Liquid

General info

Function

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

Post-translational modifications

N- and O-glycosylated.

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was previously labelled as Entactin.

Supplementary info

Biological summary

Entactin interacts closely with laminin and type IV collagen within the extracellular matrix forming an important part of the basement membrane complex. This protein aids in the stabilization and linkage of these matrices playing an essential role in cellular adhesion and migration processes. By facilitating the structural assembly of basement membranes entactin supports cellular signaling pathways that are involved in tissue development and repair.

Mechanical summary

Entactin also known as nidogen-1 or NID1 is a significant glycoprotein of the extracellular matrix with a molecular mass of approximately 150 kDa. This protein is often referred to as nidogen and nidogen 1. It localizes mainly in basement membranes across various tissues. Entactin serves as an important structural component and mediator between other extracellular molecules contributing significantly to the maintenance and integrity of the basement membrane structure.

Pathway

Entactin acts within the integrin signaling pathway and the basement membrane formation pathway. It interacts with integrin receptors on the cell surface thereby influencing processes like cell adhesion and morphogenesis. Through these pathways entactin signals in coordination with laminins and collagens building a robust structural framework necessary for tissue integrity and function.

Disease

Entactin's disruption has links to cancer and certain genetic disorders affecting basement membrane integrity. Aberrant expression or dysfunction of entactin can contribute to the progression of tumor invasion due to its pivotal role in cellular adhesion. Furthermore it associates with muscular dystrophies through interactions with laminins and collagens highlighting its importance in maintaining normal tissue architecture and function.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Terms & Conditions.

1 product image

  • SDS-PAGE - Recombinant Human Entactin/NID protein (ab158997), expandable thumbnail

    SDS-PAGE - Recombinant Human Entactin/NID protein (ab158997)

    ab158997 on a 12.5% SDS-PAGE stained with Coomassie Blue.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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