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AB127104

Recombinant Human ENTPD4 protein

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Recombinant Human ENTPD4 protein is a Human Fragment protein, in the 374 to 551 aa range, expressed in Escherichia coli, with 85%, suitable for SDS-PAGE.

View Alternative Names

KIAA0392, LALP70, LYSAL1, ENTPD4, Ectonucleoside triphosphate diphosphohydrolase 4, NTPDase 4, Golgi UDPase, Lysosomal apyrase-like protein of 70 kDa, Uridine-diphosphatase, UDPase

Key facts

Purity

85% SDS-PAGE

Expression system

Escherichia coli

Tags

His-DHFR tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9Y227

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"21 kDa","actualMolecularWeight":null,"aminoAcidEnd":551,"aminoAcidStart":374,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9Y227","tags":[{"tag":"His-DHFR","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENTPD4 also known as ectonucleoside triphosphate diphosphohydrolase 4 is an enzyme involved in the hydrolysis of nucleoside triphosphates and diphosphates into monophosphates. This enzyme has an approximate molecular mass of 55 kDa. ENTPD4 is ubiquitously expressed with significant levels detected in the liver and brain tissues which indicates its broad functional importance in various physiological processes.
Biological function summary

ENTPD4 participates in the phosphorylation cycle by regulating nucleotide levels within the cell. This enzyme is part of the ectonucleoside triphosphate diphosphohydrolase family which plays a role in extracellular nucleotide metabolism. ENTPD4 influences cellular signaling processes by modulating the concentrations of substrates and products in these reactions which facilitates proper cellular communication and metabolic regulation.

Pathways

ENTPD4 contributes significantly to ATP catabolism and purinergic signaling pathways. Within the purinergic signaling pathway ENTPD4 influences the availability of extracellular ATP a signaling molecule that binds to purinergic receptors affecting cellular processes such as inflammation and thrombosis. ENTPD4 is related to proteins such as ENTPD1 another enzyme in this family that similarly regulates ATP levels in the extracellular space maintaining physiological balance and cellular function.

ENTPD4 has connections with diseases affecting the nervous system and metabolic disorders. Variations in ENTPD4 expression or function may contribute to neurodegenerative diseases where nucleotide imbalance plays a role in neuronal damage. Additionally disorders like hyperuricemia have a relation with ENTPD4 activity as purine metabolism is affected. ENTPD4 shows correlations with proteins like ADA which also partake in nucleotide metabolism and can influence disease progression in these contexts.
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Specifications

Form

Lyophilized

Additional notes

Purified via His tag

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General info

Function

Isoform 1. Catalyzes the hydrolysis of nucleoside triphosphates and diphosphates in a calcium- or magnesium-dependent manner, with a preference for pyrimidines. Preferentially hydrolyzes UTP and TTP. AMP, ADP, ATP and UMP are not substrates (PubMed : 10858452, PubMed : 9556635). Preferentially activated by Ca(2+) over Mg(2+) (PubMed : 10858452).. Isoform 2. Has a broad substrate specificity with the ability of cleaving all nucleotide di- and triphosphates with the exception of adenosine di- and triphosphate (ADP and ATP). Preferentially hydrolyzes CTP, UDP, CDP, GTP and GDP. Can use either Ca(2+) or Mg(2+) equally.

Sequence similarities

Belongs to the GDA1/CD39 NTPase family.

Subcellular localisation

Lysosome membrane

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Product protocols

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Target data

Isoform 1. Catalyzes the hydrolysis of nucleoside triphosphates and diphosphates in a calcium- or magnesium-dependent manner, with a preference for pyrimidines. Preferentially hydrolyzes UTP and TTP. AMP, ADP, ATP and UMP are not substrates (PubMed : 10858452, PubMed : 9556635). Preferentially activated by Ca(2+) over Mg(2+) (PubMed : 10858452).. Isoform 2. Has a broad substrate specificity with the ability of cleaving all nucleotide di- and triphosphates with the exception of adenosine di- and triphosphate (ADP and ATP). Preferentially hydrolyzes CTP, UDP, CDP, GTP and GDP. Can use either Ca(2+) or Mg(2+) equally.
See full target information ENTPD4
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Primary Antibodies

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