JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB114654

Recombinant Human EPB41 protein (GST tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human EPB41 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 720 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

E41P, EPB41, Protein 4.1, P4.1, 4.1R, Band 4.1, EPB4.1, Erythrocyte membrane protein band 4.1

1 Images
SDS-PAGE - Recombinant Human EPB41 protein (GST tag N-Terminus) (AB114654)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human EPB41 protein (GST tag N-Terminus) (AB114654)

12.5% SDS-PAGE showing ab114654 at approximately 105.27kDa stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, ELISA, WB

applications

Biologically active

No

Accession

P11171

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

style
left-column

Complementary Products

Primary Antibodies

AB185704

Anti-EPB41 antibody

Immunocytochemistry/ Immunofluorescence - Anti-EPB41 antibody (AB185704)

  • 0
  • 0
Proteins & Peptides

AB268846

Recombinant Human NKF3 kinase family member/PEAK1 protein (Tagged)

SDS-PAGE - Recombinant Human NKF3 kinase family member/PEAK1 protein (Tagged) (AB268846)

  • 0
  • 0
Primary Antibodies

AB126617

Anti-SHBG antibody [EPR6628]

RabMAb|Recombinant

Western blot - Anti-SHBG antibody [EPR6628] (AB126617)

  • 0
  • 0
Proteins & Peptides

AB224853

Recombinant Human PZP protein (His tag)

SDS-PAGE - Recombinant Human PZP protein (His tag) (AB224853)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein).</p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MTTEKSLVTEAENSQHQQKEEGEEAINSGQQEPQQEESCQTAAEGDNWCEQKLKASNGDTPTHEDLTKNKERTSESRGLSRLFSSFLKRPKSQVSEEEGKEVESDKEKGEGGQKEIEFGTSLDEEIILKAPIAAPEPELKTDPSLDLHSLSSAETQPAQEELREDPDFEIKEGEGLEECSKIEVKEESPQSKAETELKASQKPIRKHRNMHCKVSLLDDTVYECVVEKHAKGQDLLKRVCEHLNLLEEDYFGLAIWDNATSKTWLDSAKEIKKQVRGVPWNFTFNVKFYPPDPAQLTEDITRYYLCLQLRQDIVAGRLPCSFATLALLGSYTIQSELGDYDPELHGVDYVSDFKLAPNQTKELEEKVMELHKSYRSMTPAQADLEFLENAKKLSMYGVDLHKAKDLEGVDIILGVCSSGLLVYKDKLRINRFPWPKVLKISYKRSSFFIKIRPGEQEQYESTIGFKLPSYRAAKKLWKVCVEHHTFFRLTSTDTIPKSKFLALGSKFRYSGRTQAQTRQASALIDRPAPHFERTASKRASRSLDGAAAVDSADRSPRPTSAPAITQGQVAEGGVLDASAKKTVVPKAQKETVKAEVKKEDEPPEQAEPEPTEAWKVEKTHIEVTVPTSNGDQTQKKRERLDGENIYIRHSNLMLEDLDKSQEEIKKHHASISELKKNFMESVPEPRPSEWDKRLSTHSPFRTLNINGQIPTGEGVSTLST","proteinLength":"Full Length","predictedMolecularWeight":"105.27 kDa","actualMolecularWeight":null,"aminoAcidEnd":720,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P11171","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein 4.1 also known as EPB41 plays an important role in maintaining the mechanical stability and elasticity of the erythrocyte membrane. This protein exists as a component of the erythrocyte membrane skeleton where it interacts with spectrin and actin contributing to the cytoskeletal network. It weighs approximately 80kDa. EPB41 expression is not limited to erythrocytes; it's found in various tissues including the brain and kidney which indicates its diverse functional roles.
Biological function summary

The protein supports cell shape and mechanical properties by linking the cytoskeleton to the plasma membrane. EPB41 is involved in the membrane-cytoskeletal junctional complex where it interacts with other proteins such as spectrin and glycophorin C. This interaction is key in erythrocytes to prevent cell lysis under mechanical stress. Beyond its role in erythrocytes EPB41 assists in protein stabilization and localization within the plasma membrane in other cell types.

Pathways

EPB41 plays an important part in the erythrocyte cell surface structure pathway as well as cell signaling pathways in non-erythroid cells. In the former EPB41 works closely with spectrin and actin to maintain the cellular integrity necessary for oxygen transport. Furthermore in cell signaling pathways its interaction with ankyrin influences membrane organization and signal transduction which are vital for diverse cellular functions.

Mutations or malfunctions in EPB41 correlate with conditions such as hereditary elliptocytosis and neurodegenerative disorders. Hereditary elliptocytosis results from defects in erythrocyte membrane proteins where EPB41 interplays with spectrin to contribute to the disorder. In neurodegenerative diseases alterations in EPB41 may affect brain tissue stability and function due to its role in sustaining membrane mechanics.
style
left-column

Specifications

Form

Solid

style
left-column

General info

Function

Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase (PubMed : 23870127).

Post-translational modifications

Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions.. Phosphorylation on Tyr-660 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex.. O-glycosylated; contains N-acetylglucosamine side chains in the C-terminal domain.

style
left-column

Product protocols

style
left-column

Target data

Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase (PubMed : 23870127).
See full target information EPB41
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com