Recombinant Human EpCAM protein (His tag) is a Human Fragment protein, in the 24 to 265 aa range, expressed in Baculovirus, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
Q E E C V C E N Y K L A V N C F V N N N R Q C Q C T S V G A Q N T V I C S K L A A K C L V M K A E M N G S K L G R R A K P E G A L Q N N D G L Y D P D C D E S G L F K A K Q C N G T S T C W C V N T A G V R R T D K D T E I T C S E R V R T Y W I I I E L K H K A R E K P Y D S K S L R T A L Q K E I T T R Y Q L D P K F I T S I L Y E N N V I T I D L V Q N S S Q K T Q N D V D I A D V A Y Y F E K D V K G E S L F H S K K M D L T V N G E Q L D L D P G Q T L I Y Y V D E K A P E F S M Q G L K H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Alternative names
CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314
Recommended products
Recombinant Human EpCAM protein (His tag) is a Human Fragment protein, in the 24 to 265 aa range, expressed in Baculovirus, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P16422-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- Q E E C V C E N Y K L A V N C F V N N N R Q C Q C T S V G A Q N T V I C S K L A A K C L V M K A E M N G S K L G R R A K P E G A L Q N N D G L Y D P D C D E S G L F K A K Q C N G T S T C W C V N T A G V R R T D K D T E I T C S E R V R T Y W I I I E L K H K A R E K P Y D S K S L R T A L Q K E I T T R Y Q L D P K F I T S I L Y E N N V I T I D L V Q N S S Q K T Q N D V D I A D V A Y Y F E K D V K G E S L F H S K K M D L T V N G E Q L D L D P G Q T L I Y Y V D E K A P E F S M Q G L K H H H H H H
- Accession
- P16422
- Protein length
- Fragment
- Predicted molecular weight
- 28.2 kDa
- Amino acids
- 24 to 265
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
- Sequence similarities
Belongs to the EPCAM family.
- Post-translational modifications
Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
- Biological function summary
EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.
- Pathways
EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.
- Associated diseases and disorders
EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
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1 product image
SDS-PAGE - Recombinant Human EpCAM protein (His tag) (ab270570)
SDS-PAGE analysis of 3 μg of ab270570.
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