Recombinant human Eph receptor A1/EphA1 protein is a Human Fragment protein, in the 613 to 892 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
L D F T R E L D P A W L M V D T V I G E G E F G E V Y R G T L R L P S Q D C K T V A I K T L K D T S P G G Q W W N F L R E A T I M G Q F S H P H I L H L E G V V T K R K P I M I I T E F M E N G A L D A F L R E R E D Q L V P G Q L V A M L Q G I A S G M N Y L S N H N Y V H R D L A A R N I L V N Q N L C C K V S D F G L T R L L D D F D G T Y E T Q G G K I P I R W T A P E A I A H R I F T T A S D V W S F G I V M W E V L S F G D K P Y G E M S N Q E V M K S I E D G Y R L P P P V D C P A P L Y E L M K N C W A Y D R A R R P H F Q K L Q A H L E Q L L A N P H S L R T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Also plays a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.
Alternative names
EPH, EPHT, EPHT1, EPHA1, Ephrin type-A receptor 1, hEpha1, EPH tyrosine kinase, EPH tyrosine kinase 1, Erythropoietin-producing hepatoma receptor, Tyrosine-protein kinase receptor EPH
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Recombinant human Eph receptor A1/EphA1 protein is a Human Fragment protein, in the 613 to 892 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab206006 was determined to be 20 nmol/min/mg.
- Accession
- P21709-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- L D F T R E L D P A W L M V D T V I G E G E F G E V Y R G T L R L P S Q D C K T V A I K T L K D T S P G G Q W W N F L R E A T I M G Q F S H P H I L H L E G V V T K R K P I M I I T E F M E N G A L D A F L R E R E D Q L V P G Q L V A M L Q G I A S G M N Y L S N H N Y V H R D L A A R N I L V N Q N L C C K V S D F G L T R L L D D F D G T Y E T Q G G K I P I R W T A P E A I A H R I F T T A S D V W S F G I V M W E V L S F G D K P Y G E M S N Q E V M K S I E D G Y R L P P P V D C P A P L Y E L M K N C W A Y D R A R R P H F Q K L Q A H L E Q L L A N P H S L R T
- Accession
- P21709
- Protein length
- Fragment
- Predicted molecular weight
- 55 kDa
- Amino acids
- 613 to 892
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Also plays a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
- Post-translational modifications
Phosphorylated. Autophosphorylation is stimulated by its ligand EFNA1.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Previously labelled as Eph receptor A1.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Eph receptor A1 also known by the alternative name EphA1 is a member of the ephrin receptor family part of the larger group of receptor tyrosine kinases. The EphA1 protein carries a molecular mass of approximately 108 kDa. Mechanically it functions as a cellular signaling molecule by binding ephrins its membrane-bound ligands which initiates bidirectional signaling pathways. This interaction typically occurs at cell-cell interfaces involving dynamic communication essential for cellular processes. EphA1 is expressed in a variety of tissues with a significant presence in epithelial tissues where it plays roles in maintaining tissue architecture.
- Biological function summary
EphA1 contributes to cellular adhesion migration and positioning linking to ephrin ligands to form signaling complexes. These complexes aid in regulating developmental processes such as angiogenesis and neuronal development by transmitting signals that control cell shape and movement. The EphA1 receptor also influences cell proliferation and plays a role in the inhibition of cell migration by responding to concentration gradients of ephrin ligands. Its ability to control cellular boundaries and morphology highlights its importance in tissue patterning and organogenesis.
- Pathways
EphA1 integrates into the Eph/ephrin signaling pathway a critical framework for developmental processes and cellular organization. This pathway involves cross-talk with the MAPK signaling cascade which further influences cell proliferation and differentiation. The interplay between EphA1 and other Eph receptors like EphB2 enhances bidirectional signaling that accommodates both forward and reverse signal transduction a feature that accommodates dynamic cellular responses in development and homeostasis.
- Associated diseases and disorders
EphA1 has significant connections to cancer progression and metastasis particularly in colorectal carcinoma and melanoma. Alterations in EphA1 expression can lead to deregulated cellular pathways contributing to tumor development and spread. EphA1's relationship with proteins like ephrin A1 and other Eph receptors such as EphA2 provides a link between its dysregulation and cancer pathology highlighting its potential as a therapeutic target in oncology.
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2 product images
SDS-PAGE - Recombinant human Eph receptor A1/EphA1 protein (ab206006)
SDS-PAGE analysis of ab206006 by densitometry, approximate MW 55 kDa.
Functional Studies - Recombinant human Eph receptor A1/EphA1 protein (ab206006)
The specific activity of ab206006 was determined to be 20 nmol/min/mg.
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