Recombinant human Eph receptor A4/SEK protein is a Human Fragment protein, in the 570 to 986 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
S R R R S K Y S K A K Q E A D E E K H L N Q G V R T Y V D P F T Y E D P N Q A V R E F A K E I D A S C I K I E K V I G V G E F G E V C S G R L K V P G K R E I C V A I K T L K A G Y T D K Q R R D F L S E A S I M G Q F D H P N I I H L E G V V T K C K P V M I I T E Y M E N G S L D A F L R K N D G R F T V I Q L V G M L R G I G S G M K Y L S D M S Y V H R D L A A R N I L V N S N L V C K V S D F G M S R V L E D D P E A A Y T T R G G K I P I R W T A P E A I A Y R K F T S A S D V W S Y G I V M W E V M S Y G E R P Y W D M S N Q D V I K A I E E G Y R L P P P M D C P I A L H Q L M L D C W Q K E R S D R P K F G Q I V N M L D K L I R N P N S L K R T G T E S S R P N T A L L D P S S P E F S A V V S V G D W L Q A I K M D R Y K D N F T A A G Y T T L E A V V H V N Q E D L A R I G I T A I T H Q N K I L S S V Q A M R T Q M Q Q M H G R M V P V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, also plays a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium. During development of the cochlear organ of Corti, regulates pillar cell separation by forming a ternary complex with ADAM10 and CADH1 which facilitates the cleavage of CADH1 by ADAM10 and disruption of adherens junctions (By similarity). Phosphorylates CAPRIN1, promoting CAPRIN1-dependent formation of a membraneless compartment (By similarity).
Alternative names
HEK8, SEK, TYRO1, EPHA4, Ephrin type-A receptor 4, EPH-like kinase 8, Tyrosine-protein kinase TYRO1, Tyrosine-protein kinase receptor SEK, EK8, hEK8
Recommended products
Recombinant human Eph receptor A4/SEK protein is a Human Fragment protein, in the 570 to 986 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab206013 was determined to be 22 nmol /min/mg.
- Accession
- P54764-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- S R R R S K Y S K A K Q E A D E E K H L N Q G V R T Y V D P F T Y E D P N Q A V R E F A K E I D A S C I K I E K V I G V G E F G E V C S G R L K V P G K R E I C V A I K T L K A G Y T D K Q R R D F L S E A S I M G Q F D H P N I I H L E G V V T K C K P V M I I T E Y M E N G S L D A F L R K N D G R F T V I Q L V G M L R G I G S G M K Y L S D M S Y V H R D L A A R N I L V N S N L V C K V S D F G M S R V L E D D P E A A Y T T R G G K I P I R W T A P E A I A Y R K F T S A S D V W S Y G I V M W E V M S Y G E R P Y W D M S N Q D V I K A I E E G Y R L P P P M D C P I A L H Q L M L D C W Q K E R S D R P K F G Q I V N M L D K L I R N P N S L K R T G T E S S R P N T A L L D P S S P E F S A V V S V G D W L Q A I K M D R Y K D N F T A A G Y T T L E A V V H V N Q E D L A R I G I T A I T H Q N K I L S S V Q A M R T Q M Q Q M H G R M V P V
- Accession
- P54764
- Protein length
- Fragment
- Predicted molecular weight
- 73 kDa
- Amino acids
- 570 to 986
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, also plays a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium. During development of the cochlear organ of Corti, regulates pillar cell separation by forming a ternary complex with ADAM10 and CADH1 which facilitates the cleavage of CADH1 by ADAM10 and disruption of adherens junctions (By similarity). Phosphorylates CAPRIN1, promoting CAPRIN1-dependent formation of a membraneless compartment (By similarity).
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
- Subcellular localisation
- Early endosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product was previously labelled as Eph receptor A4
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Eph receptor A4 also known as SEK is a type of ephrin receptor that plays a significant role in cellular communication. It belongs to the Eph protein family and it is classified as a receptor tyrosine kinase. EphA4 has an approximate molecular mass of 110 kDa. It is mainly expressed in the nervous system although it can also be found in other tissues including the vascular and skeletal systems. This receptor has an important function in mediating signal transduction upon binding with their ephrin ligands.
- Biological function summary
EphA4 plays a critical role in developmental processes particularly in the development of the nervous system and vascular patterning. The receptor engages in bidirectional signaling meaning that signals are transmitted not only into the receptor-expressing cells but also into the ephrin-expressing ones. EphA4 is part of complexes that can include multiple receptor and ephrin molecules which allows for the modulation of cell adhesion and migration. These processes are essential for forming tissue boundaries and axon guidance.
- Pathways
EphA4 signaling is involved in two significant pathways: the Eph/ephrin signaling pathway and the cytoskeletal remodeling pathway. In the Eph/ephrin signaling pathway EphA4 interacts with various ephrin ligands leading to changes in cellular architecture and behavior. The cytoskeletal remodeling pathway involves interactions with proteins such as Rho and Ras which coordinate cytoskeletal changes necessary for cell movement and positioning. These pathways are integral to processes like axon guidance and cell migration underpinning EphA4’s role in development.
- Associated diseases and disorders
EphA4 has been implicated in conditions such as amyotrophic lateral sclerosis (ALS) and cancer metastasis. In the context of ALS EphA4's interaction with proteins like RhoA affects axonal repair and neuron survival contributing to the disease's progression. Regarding cancer EphA4 influences tumor growth and spread with its signaling pathways impacting cellular invasion and angiogenesis. Understanding these aspects of EphA4 activity assists in developing therapeutic strategies for these disorders.
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2 product images
SDS-PAGE - Recombinant human Eph receptor A4/SEK protein (ab206013)
SDS-PAGE analysis of Eph receptor A4/SEK (ab206013).
Functional Studies - Recombinant human Eph receptor A4/SEK protein (ab206013)
Sample Kinase Activity Plot. The specific activity of ab206013 was determined to be 22 nmol /min/mg.
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