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AB276448

Recombinant human Eph receptor B1/NET protein (His tag)

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Recombinant human Eph receptor B1/NET protein (His tag) is a Human Fragment protein, in the 1 to 540 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

ELK, EPHT2, HEK6, NET, EPHB1, Ephrin type-B receptor 1, EPH tyrosine kinase 2, EPH-like kinase 6, Neuronally-expressed EPH-related tyrosine kinase, Tyrosine-protein kinase receptor EPH-2, EK6, hEK6

3 Images
Functional Studies - Recombinant human Eph receptor B1/NET protein (His tag) (AB276448)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Eph receptor B1/NET protein (His tag) (AB276448)

Measured by its binding ability in a functional ELISA.

Immobilized human EPHB1-His at 10 μg/ml (100 μl/well) can bind human EFNB1-Fc2h with a linear ranger of 3.125-200 ng/mL.

Functional Studies - Recombinant human Eph receptor B1/NET protein (His tag) (AB276448)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Eph receptor B1/NET protein (His tag) (AB276448)

Measured by its binding ability in a functional ELISA.

Immobilized human EPHB1-His at 10 μg/ml (100 μl/well) can bind human EFNB2-Fch with a linear ranger of 0.3125-20 ng/mL.

SDS-PAGE - Recombinant human Eph receptor B1/NET protein (His tag) (AB276448)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Eph receptor B1/NET protein (His tag) (AB276448)

SDS-PAGE analysis of ab276448

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Measured by its binding ability in a functional ELISA.

Immobilized human EPHB1-His at 10 μg/ml (100 μl/well) can bind human EFNB1-Fc2h with a linear ranger of 3.125-200 ng/mL.

Immobilized human EPHB1-His at 10 μg/ml (100 μl/well) can bind human EFNB2-Fch with a linear ranger of 0.3125-20 ng/mL.

Accession

P54762

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 95% PBS, 5% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MALDYLLLLLLASAVAAMEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQEAEGCSHCPSNSRSPAEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLP","proteinLength":"Fragment","predictedMolecularWeight":"60 kDa","actualMolecularWeight":null,"aminoAcidEnd":540,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P54762","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Eph receptor B1 also called NET or B1 receptor is a member of the Eph receptor family. It plays roles in various cellular processes through its mechanical action as a receptor tyrosine kinase. The Eph receptor B1 primarily mediates signaling by binding to ephrin-B ligands. This interaction triggers bidirectional signaling between cells influencing cell positioning shape and movement. Eph receptor B1 which has a molecular weight of approximately 120 kDa expresses highly in tissues like the brain and testes suggesting its importance in these organ systems.
Biological function summary

Eph receptor B1 affects cell adhesion migration and tissue patterning by participating in complex networks. These receptors and their ephrin ligands form a complex that enables cross-communication between adjacent cells. Eph receptor B1 modulates processes like synapse formation in the brain thereby impacting neural development and connectivity. As a result it plays a role in maintaining cellular architecture and function in a specific tissue context.

Pathways

Eph receptor B1 is an integral part of the Eph/Ephrin signaling pathway which influences developmental processes and cellular interactions. In this pathway Eph receptor B1 works alongside proteins like ephrin-B to mediate cell-cell communication and signal transduction. This pathway has implications for the formation and remodeling of blood vessels suggesting further links to the angiogenesis pathway. Such pathways are important for translating external signals into cellular responses shaping the biology of tissues and organs.

Alterations in Eph receptor B1 function can relate to conditions such as cancer and neurological disorders. In cancers aberrant Eph receptor B1 signaling can promote tumor growth and metastasis with the receptor often associating with proteins like ephrin-B to drive tumorigenic processes. In the context of neurological diseases like Alzheimer's disease disruptions in Eph receptor B1 signaling can affect neural connectivity and function illustrating its role in maintaining brain health. Understanding these connections helps clarify the receptor's impact on disease development and progression.
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Specifications

Form

Lyophilized

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General info

Function

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance also plays an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively. Involved in the maintenance of the pool of satellite cells (muscle stem cells) by promoting their self-renewal and reducing their activation and differentiation (By similarity).

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Post-translational modifications

Phosphorylated. Autophosphorylation is stimulated by the ligand EFNB1. Required for interaction with SH2 domain-containing interactors, for activation of the MAPK/ERK and JUN signaling cascades and for ubiquitination by CBL.. Ubiquitinated; (EFNB1)ligand-induced poly- and/or multi-ubiquitination by CBL is regulated by SRC and leads to lysosomal degradation.

Subcellular localisation

Early endosome membrane

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Product protocols

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Target data

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance also plays an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively. Involved in the maintenance of the pool of satellite cells (muscle stem cells) by promoting their self-renewal and reducing their activation and differentiation (By similarity).
See full target information EPHB1
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