Recombinant human Ephrin B2 protein (Fc Chimera His Tag) is a Human Fragment protein, in the 1 to 229 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A V R R D S V W K Y C W G V L M V L C R T A I S K S I V L E P I Y W N S S N S K F L P G Q G L V L Y P Q I G D K L D I I C P K V D S K T V G Q Y E Y Y K V Y M V D K D Q A D R C T I K K E N T P L L N C A K P D Q D I K F T I K F Q E F S P N L W G L E F Q K N K D Y Y I I S T S N G S L E G L D N Q E G G V C Q T R A M K I L M K V G Q D A S S A G S T R N K D P T R R P E L E A G T N G R S S T T S P F V K P N P G S S T D G N S A G H S G N N I L G S E V A L F A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons. (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.
Alternative names
EPLG5, HTKL, LERK5, EFNB2, Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5, HTK ligand, LERK-5, HTK-L
Recommended products
Recombinant human Ephrin B2 protein (Fc Chimera His Tag) is a Human Fragment protein, in the 1 to 229 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA .
Immobilized human EphB4-his at 2 μg/ml (100 μl/well) can bind human EphrinB2 Fc chimera with a linear range of 1-25 ng/ml.
- Accession
- P52799-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M A V R R D S V W K Y C W G V L M V L C R T A I S K S I V L E P I Y W N S S N S K F L P G Q G L V L Y P Q I G D K L D I I C P K V D S K T V G Q Y E Y Y K V Y M V D K D Q A D R C T I K K E N T P L L N C A K P D Q D I K F T I K F Q E F S P N L W G L E F Q K N K D Y Y I I S T S N G S L E G L D N Q E G G V C Q T R A M K I L M K V G Q D A S S A G S T R N K D P T R R P E L E A G T N G R S S T T S P F V K P N P G S S T D G N S A G H S G N N I L G S E V A L F A
- Accession
- P52799
- Protein length
- Fragment
- Predicted molecular weight
- 50.3 kDa
- Amino acids
- 1 to 229
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus, His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
- Sequence similarities
Belongs to the ephrin family.
- Post-translational modifications
Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ephrin B2 also called EFNB2 or ephrin-B2 is a transmembrane protein that plays important roles in cell signaling. The protein weighs approximately 37 kDa and it is expressed in various tissues like endothelial cells and neuronal tissues. Ephrin B2 binds to Eph receptors which are a part of the largest family of receptor tyrosine kinases. This interaction facilitates bidirectional signaling necessary for mediating cell communication.
- Biological function summary
Ephrin-B2 serves as critical mediator for vascular development and nervous system maturation. It is an important player in angiogenesis where it functions through interaction with EphB receptors to regulate blood vessel formation and remodeling. Ephrin-B2 part of the larger ephrin family often acts in concert with other ephrins and Eph receptors to form signaling complexes that coordinate cell positioning and tissue architecture.
- Pathways
The action of ephrin-B2 integrates into the Eph/ephrin signaling pathway and the VEGF signaling pathway. These pathways are important for cellular guidance and migration particularly in developing tissues. Ephrin B2's interaction with proteins like VEGF and its receptors emphasizes its role in angiogenic processes. Furthermore Ephrin-B2 interacts with other Eph receptor proteins to modulate cellular responses essential for proper vascular and neural functions.
- Associated diseases and disorders
Ephrin-B2's dysregulation associates with cancer and cardiovascular conditions. High expression of ephrin-B2 has been observed in tumor angiogenesis linking it to cancer progression through interactions with VEGF. Abnormal ephrin-B2 signaling may also relate to atherosclerosis involving disrupted endothelial cell function. These associations reveal ephrin-B2's potential as a therapeutic target for conditions involving aberrant vascular and cellular dynamics.
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2 product images
Functional Studies - Recombinant human Ephrin B2 protein (Fc Chimera His Tag) (ab276332)
Measured by its binding ability in a functional ELISA .
Immobilized human EphB4-his at 2 μg/ml (100 μl/well) can bind human EphrinB2 Fc chimera with a linear range of 1-25 ng/ml.
SDS-PAGE - Recombinant human Ephrin B2 protein (Fc Chimera His Tag) (ab276332)
SDS-PAGE analysis of ab276332
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