Recombinant Human Ephrin B3 protein (denatured) is a Human Fragment protein, in the 28 to 226 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M L S L E P V Y W N S A N K R F Q A E G G Y V L Y P Q I G D R L D L L C P R A R P P G P H S S P N Y E F Y K L Y L V G G A Q G R R C E A P P A P N L L L T C D R P D L D L R F T I K F Q E Y S P N L W G H E F R S H H D Y Y I I A T S D G T R E G L E S L Q G G V C L T R G M K V L L R V G Q S P R G G A V P R K P V S E M P M E R D R G A A H S L E P G K E N L P G D P T S N A T S R G A E G P L P P P S M P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity). (Microbial infection) Acts as a receptor for nipah virus and hendra virus.
Alternative names
EPLG8, LERK8, EFNB3, Ephrin-B3, EPH-related receptor transmembrane ligand ELK-L3, EPH-related receptor tyrosine kinase ligand 8, LERK-8
Recommended products
Recombinant Human Ephrin B3 protein (denatured) is a Human Fragment protein, in the 28 to 226 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q15768-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 12.01% Urea, 0.58% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M L S L E P V Y W N S A N K R F Q A E G G Y V L Y P Q I G D R L D L L C P R A R P P G P H S S P N Y E F Y K L Y L V G G A Q G R R C E A P P A P N L L L T C D R P D L D L R F T I K F Q E Y S P N L W G H E F R S H H D Y Y I I A T S D G T R E G L E S L Q G G V C L T R G M K V L L R V G Q S P R G G A V P R K P V S E M P M E R D R G A A H S L E P G K E N L P G D P T S N A T S R G A E G P L P P P S M P
- Accession
- Q15768
- Protein length
- Fragment
- Predicted molecular weight
- 24.6 kDa
- Amino acids
- 28 to 226
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).
- Sequence similarities
Belongs to the ephrin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ephrin B3 also known as EFNB3 is a critical membrane-bound ligand for the Eph receptor tyrosine kinases. EFNB3 belongs to the ephrin family with a molecular mass of approximately 37 kDa. It predominantly expresses in the brain although researchers have detected it in other tissues as well. The protein plays a significant role in various cellular processes such as cell adhesion shape and migration.
- Biological function summary
EFNB3 influences axon guidance and neural development. As part of a receptor-ligand complex it interacts with Eph receptors to regulate synaptic plasticity which is essential for learning and memory. This interaction also plays a role in mediating repulsive and attractive signals that guide neurons during developmental processes. EFNB3 exhibits distinct binding characteristics which determine specific cellular responses.
- Pathways
EFNB3 influences both the ephrin signaling pathway and the nervous system development pathway. It engages with Eph receptors particularly EphB3 and EphA4 to trigger downstream signaling cascades that influence cellular structure and fate. The Eph-ephrin pathways are important in neurodevelopmental processes assisting in the formation of spatially regulated cell organization.
- Associated diseases and disorders
EFNB3 has connections to neurodegenerative diseases and cancer. Abnormal EFNB3 signaling can contribute to Alzheimer's Disease due to its role in neuronal connectivity and communication. The protein also associates with glioblastoma as it can influence tumor cell invasiveness and growth. Changes in ephrin signaling can alter normal cellular behavior which may progress to various pathological conditions.
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1 product image
SDS-PAGE - Recombinant Human Ephrin B3 protein (denatured) (ab130026)
ab130026 at 3μg analysed by 15% SDS-PAGE.
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