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AB277025

Recombinant Human EPO-R + IL-3RB protein (Fc Chimera)

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Recombinant Human EPO-R + IL-3RB protein (Fc Chimera) is a Human Fragment protein, in the 17 to 443 aa range, expressed in HEK 293 cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

Erythropoietin receptor, EPO-R, EPOR, CD131, IL3RB, IL5RB, CSF2RB, Cytokine receptor common subunit beta, CDw131, GM-CSF/IL-3/IL-5 receptor common beta subunit

1 Images
SDS-PAGE - Recombinant Human EPO-R + IL-3RB protein (Fc Chimera) (AB277025)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human EPO-R + IL-3RB protein (Fc Chimera) (AB277025)

SDS-PAGE analysis of ab277025

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P19235

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"WERSLAGAEETIPLQTLRCYNDYTSHITCRWADTQDAQRLVNVTLIRRVNEDLLEPVSCDLSDDMPWSACPHPRCVPRRCVIPCQSFVVTDVDYFSFQPDRPLGTRLTVTLTQHVQPPEPRDLQISTDQDHFLLTWSVALGSPQSHWLSPGDLEFEVVYKRLQDSWEDAAILLSNTSQATLGPEHLMPSSTYVARVRTRLAPGSRLSGRPSKWSPEVCWDSQPGDEAQPQNLECFFDGAAVLSCSWEVRKEVASSVSFGLFYKPSPDAGEEECSPVLREGLGSLHTRHHCQIPVPDPATHGQYIVSVQPRRAEKHIKSSVNIQMAPPSLNVTKDGDSYSLRWETMKMRYEHIDHTFEIQYRKDTATWKDSKTETLQNAHSMALPALEPSTRYWARVRVRTSRTGYNGIWSEWSEARSWDTESVLPMW","proteinLength":"Fragment","predictedMolecularWeight":"101 kDa","actualMolecularWeight":null,"aminoAcidEnd":443,"aminoAcidStart":17,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P32927","tags":[{"tag":"Fc","terminus":"C-Terminus"}]},{"linker":null,"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"101 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P19235","tags":[{"tag":"Fc","terminus":"C-Terminus"}]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EPO-R also known as erythropoietin receptor is a cell surface receptor primarily involved in erythropoiesis. The receptor has a molecular weight of around 55 kDa. It is expressed predominantly in erythroid progenitor cells and also in some non-hematopoietic tissues. IL-3RB another component of this receptor complex helps form high-affinity binding sites for erythropoietin on these cells.
Biological function summary

EPO-R operates as part of a receptor complex that is critical in promoting the survival proliferation and differentiation of erythroid progenitors. It forms a functional unit by associating with the JAK2 kinase which gets activated upon ligand binding. This activation leads to the initiation of signaling cascades that result in the production of red blood cells. IL-3RB cooperates with other cytokine receptors like GM-CSF and IL-3 adding to the complexity of cell signaling.

Pathways

EPO-R plays a significant role in the Janus kinase (JAK) and signal transducer and activator of transcription (STAT) pathway. This pathway is essential for transferring signals from the cell membrane to the nucleus ultimately influencing gene expression. The receptor's interaction with proteins such as JAK2 and STAT5 is important for the cascade that impacts erythroid cell functions and hematopoietic regulation.

EPO-R is associated with conditions such as anemia and polycythemia. In anemia insufficient EPO-R signaling leads to reduced red blood cell production contributing to fatigue and weakness. In polycythemia excessive activation of EPO-R can result in an over-production of red blood cells increasing the risk of blood clots. Both conditions can be assessed by investigating EPO-R interactions with proteins like JAK2 which can be mutated in such disorders.
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General info

Function

Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation (PubMed : 10388848, PubMed : 2163695, PubMed : 2163696, PubMed : 8662939, PubMed : 9774108). Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade (By similarity). In some cell types, can also activate STAT1 and STAT3 (PubMed : 11756159). May also activate the LYN tyrosine kinase (By similarity).. Isoform EPOR-T. Acts as a dominant-negative receptor of EPOR-mediated signaling.

Sequence similarities

Belongs to the type I cytokine receptor family. Type 1 subfamily.

Post-translational modifications

On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2 (PubMed:11781573). The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation (PubMed:10374881). Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11 (By similarity). Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site (PubMed:12027890).. Ubiquitinated by the ECS(SOCS2) complex following ligand-binding and phosphorylation by JAK2, leading to its degradation by the proteasome (PubMed:11781573, PubMed:31182716). Regulation by the ECS(SOCS2) complex acts as a negative feedback loop of erythropoietin-mediated signaling pathway (PubMed:11781573). Ubiquitination at Lys-281 mediates receptor internalization, whereas ubiquitination at Lys-453 promotes trafficking of activated receptors to the lysosomes for degradation (By similarity). Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated (PubMed:12746455). Ubiquitination mediates proliferation and survival of EPO-dependent cells (By similarity).

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Product protocols

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Target data

Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation (PubMed : 10388848, PubMed : 2163695, PubMed : 2163696, PubMed : 8662939, PubMed : 9774108). Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade (By similarity). In some cell types, can also activate STAT1 and STAT3 (PubMed : 11756159). May also activate the LYN tyrosine kinase (By similarity).. Isoform EPOR-T. Acts as a dominant-negative receptor of EPOR-mediated signaling.
See full target information EPOR

Additional targets

CSF2RB
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