Skip to main content

Recombinant Human EPO-R protein is a Human Fragment protein, in the 25 to 250 aa range, expressed in Insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

Be the first to review this product! Submit a review

Images

SDS-PAGE - Recombinant Human EPO-R protein (AB211321), expandable thumbnail

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Insect cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

A P P P N L P D P K F E S K A A L L A A R G P E E L L C F T E R L E D L V C F W E E A A S A G V G P G N Y S F S Y Q L E D E P W K L C R L H Q A P T A R G A V R F W C S L P T A D T S S F V P L E L R V T A A S G A P R Y H R V I H I N E V V L L D A P V G L V A R L A D E S G H V V L R W L P P P E T P M T S H I R Y E V D V S A G N G A G S V Q R V E I L E G R T E C V L S N L R G R T R Y T F A V R A R M A E P S F G G F W S A W S E P V S L L T P S D L D P H H H H H H

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation (PubMed:10388848, PubMed:2163695, PubMed:2163696, PubMed:8662939, PubMed:9774108). Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade (By similarity). In some cell types, can also activate STAT1 and STAT3 (PubMed:11756159). May also activate the LYN tyrosine kinase (By similarity).Isoform EPOR-TActs as a dominant-negative receptor of EPOR-mediated signaling.

Alternative names

Recommended products

Recombinant Human EPO-R protein is a Human Fragment protein, in the 25 to 250 aa range, expressed in Insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Insect cells

Applications

SDS-PAGE

Accession
P19235-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)

Sequence info

Amino acid sequence

A P P P N L P D P K F E S K A A L L A A R G P E E L L C F T E R L E D L V C F W E E A A S A G V G P G N Y S F S Y Q L E D E P W K L C R L H Q A P T A R G A V R F W C S L P T A D T S S F V P L E L R V T A A S G A P R Y H R V I H I N E V V L L D A P V G L V A R L A D E S G H V V L R W L P P P E T P M T S H I R Y E V D V S A G N G A G S V Q R V E I L E G R T E C V L S N L R G R T R Y T F A V R A R M A E P S F G G F W S A W S E P V S L L T P S D L D P H H H H H H

Accession

P19235

Protein length

Fragment

Predicted molecular weight

25.6 kDa

Amino acids

25 to 250

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Liquid

Additional notes

Affinity purified

General info

Function

Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation (PubMed:10388848, PubMed:2163695, PubMed:2163696, PubMed:8662939, PubMed:9774108). Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade (By similarity). In some cell types, can also activate STAT1 and STAT3 (PubMed:11756159). May also activate the LYN tyrosine kinase (By similarity).

Sequence similarities

Belongs to the type I cytokine receptor family. Type 1 subfamily.

Post-translational modifications

On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2 (PubMed:11781573). The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation (PubMed:10374881). Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11 (By similarity). Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site (PubMed:12027890).

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Previously labelled as EPO Receptor.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Erythropoietin receptor (EPO-R) also known as EPOR plays an important role in erythropoiesis by mediating the effects of erythropoietin (EPO). EPO-R is a member of the cytokine receptor family with a mass of approximately 55 kDa. This receptor can primarily be found on the surface of erythroid progenitor cells in the bone marrow. Its presence is important for the final differentiation of these cells into mature erythrocytes.

Biological function summary

EPO-R functions by dimerizing upon EPO binding subsequently activating intracellular signaling pathways. It is a component of a larger signaling complex that recruits and activates various molecules initiating a cascade of events critical for cell survival and proliferation. This receptor signaling prevents apoptosis in erythroid progenitor cells promoting their growth and differentiation into red blood cells.

Pathways

EPO-R's activity falls within the JAK-STAT and PI3K-AKT signaling pathways. These pathways are pivotal in regulating hematopoiesis and cell survival. JAK2 acts as a direct partner to EPO-R and transduces the signal downstream while molecules like STAT5 get phosphorylated and mediate transcription of target genes. The PI3K-AKT pathway also contributes to the anti-apoptotic effect essential for erythrocyte development.

Associated diseases and disorders

Altered EPO-R signaling associates with anemia of chronic disease and polycythemia vera. Anemic conditions often show reduced sensitivity or expression of EPO-R while mutations leading to EPO-R hypersensitivity link to polycythemia vera. Dysregulation of related proteins such as JAK2 also contributes to these conditions indicating the interconnected roles of these proteins in blood-related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • SDS-PAGE - Recombinant Human EPO-R protein (ab211321), expandable thumbnail

    SDS-PAGE - Recombinant Human EPO-R protein (ab211321)

    15% SDS-PAGE analysis of ab211321 (3μg). Molecular weight is 28-40kDa on SDS-PAGE under reducing conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com