Recombinant human EPO-R protein (Active) is a Human Fragment protein, in the 25 to 250 aa range, expressed in Baculovirus infected insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
A P P P N L P D P K F E S K A A L L A A R G P E E L L C F T E R L E D L V C F W E E A A S A G V G P G N Y S F S Y Q L E D E P W K L C R L H Q A P T A R G A V R F W C S L P T A D T S S F V P L E L R V T A A S G A P R Y H R V I H I N E V V L L D A P V G L V A R L A D E S G H V V L R W L P P P E T P M T S H I R Y E V D V S A G N G A G S V Q R V E I L E G R T E C V L S N L R G R T R Y T F A V R A R M A E P S F G G F W S A W S E P V S L L T P S D L D P H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation (PubMed:10388848, PubMed:2163695, PubMed:2163696, PubMed:8662939, PubMed:9774108). Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade (By similarity). In some cell types, can also activate STAT1 and STAT3 (PubMed:11756159). May also activate the LYN tyrosine kinase (By similarity). Isoform EPOR-T. Acts as a dominant-negative receptor of EPOR-mediated signaling.
Alternative names
Erythropoietin receptor, EPO-R, EPOR
Recommended products
Recombinant human EPO-R protein (Active) is a Human Fragment protein, in the 25 to 250 aa range, expressed in Baculovirus infected insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its ability to inhibit EPO dependent proliferation using TF-1 human erythroleukemic cells. The ED50 for this effect is less or equal to 70ng/ml.
- Accession
- P19235-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A P P P N L P D P K F E S K A A L L A A R G P E E L L C F T E R L E D L V C F W E E A A S A G V G P G N Y S F S Y Q L E D E P W K L C R L H Q A P T A R G A V R F W C S L P T A D T S S F V P L E L R V T A A S G A P R Y H R V I H I N E V V L L D A P V G L V A R L A D E S G H V V L R W L P P P E T P M T S H I R Y E V D V S A G N G A G S V Q R V E I L E G R T E C V L S N L R G R T R Y T F A V R A R M A E P S F G G F W S A W S E P V S L L T P S D L D P H H H H H H
- Accession
- P19235
- Protein length
- Fragment
- Predicted molecular weight
- 25.6 kDa
- Amino acids
- 25 to 250
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation (PubMed:10388848, PubMed:2163695, PubMed:2163696, PubMed:8662939, PubMed:9774108). Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade (By similarity). In some cell types, can also activate STAT1 and STAT3 (PubMed:11756159). May also activate the LYN tyrosine kinase (By similarity).
- Sequence similarities
Belongs to the type I cytokine receptor family. Type 1 subfamily.
- Post-translational modifications
On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2 (PubMed:11781573). The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation (PubMed:10374881). Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11 (By similarity). Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site (PubMed:12027890).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Previously labelled as EPO Receptor.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Erythropoietin receptor (EPO-R) also known as EPOR plays an important role in erythropoiesis by mediating the effects of erythropoietin (EPO). EPO-R is a member of the cytokine receptor family with a mass of approximately 55 kDa. This receptor can primarily be found on the surface of erythroid progenitor cells in the bone marrow. Its presence is important for the final differentiation of these cells into mature erythrocytes.
- Biological function summary
EPO-R functions by dimerizing upon EPO binding subsequently activating intracellular signaling pathways. It is a component of a larger signaling complex that recruits and activates various molecules initiating a cascade of events critical for cell survival and proliferation. This receptor signaling prevents apoptosis in erythroid progenitor cells promoting their growth and differentiation into red blood cells.
- Pathways
EPO-R's activity falls within the JAK-STAT and PI3K-AKT signaling pathways. These pathways are pivotal in regulating hematopoiesis and cell survival. JAK2 acts as a direct partner to EPO-R and transduces the signal downstream while molecules like STAT5 get phosphorylated and mediate transcription of target genes. The PI3K-AKT pathway also contributes to the anti-apoptotic effect essential for erythrocyte development.
- Associated diseases and disorders
Altered EPO-R signaling associates with anemia of chronic disease and polycythemia vera. Anemic conditions often show reduced sensitivity or expression of EPO-R while mutations leading to EPO-R hypersensitivity link to polycythemia vera. Dysregulation of related proteins such as JAK2 also contributes to these conditions indicating the interconnected roles of these proteins in blood-related diseases.
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1 product image
SDS-PAGE - Recombinant human EPO-R protein (Active) (ab219430)
15% SDS-PAGE analysis of 3 μg ab219430.
Molecular Weight: 28-40 kDa (SDS-PAGE under reducing conditions).
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