Recombinant human EPO-R protein (Fc Chimera) is a Human Fragment protein, in the 25 to 250 aa range, expressed in HEK 293, with >95% purity and suitable for FuncS, SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- FuncS, SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
T h e o r e t i c a l s e q u e n c e : A P P P N L P D P K F E S K A A L L A A R G P E E L L C F T E R L E D L V C F W E E A A S A G V G P G N Y S F S Y Q L E D E P W K L C R L H Q A P T A R G A V R F W C S L P T A D T S S F V P L E L R V T A A S G A P R Y H R V I H I N E V V L L D A P V G L V A R L A D E S G H V V L R W L P P P E T P M T S H I R Y E V D V S A G N G A G S V Q R V E I L E G R T E C V L S N L R G R T R Y T F A V R A R M A E P S F G G F W S A W S E P V S L L T P S D L D P R I P K V D K K V E P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C R V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes ab84001 migrates as a broad band between 50 and 65 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted molecular mass of 52 kDa. ab84001 consists of 0-20% carbohydrate by weight. The unmodified protein has a predicted pI of 6.4. |
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Target data
Function
Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation (PubMed:10388848, PubMed:2163695, PubMed:2163696, PubMed:8662939, PubMed:9774108). Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade (By similarity). In some cell types, can also activate STAT1 and STAT3 (PubMed:11756159). May also activate the LYN tyrosine kinase (By similarity). Isoform EPOR-T. Acts as a dominant-negative receptor of EPOR-mediated signaling.
Alternative names
Erythropoietin receptor, EPO-R, EPOR
Recommended products
Recombinant human EPO-R protein (Fc Chimera) is a Human Fragment protein, in the 25 to 250 aa range, expressed in HEK 293, with >95% purity and suitable for FuncS, SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- FuncS, SDS-PAGE
- Biologically active
- Yes
- Biological activity
- The ED50 of ab84001 is typically 0.15-2.5 ng/ml as measured by its ability to neutralize EPO-mediated proliferation of TF-1 cells.
- Accession
- P19235-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in 0.5 mL of PBS
- Concentration
- Storage buffer
Constituents: 10% Trehalose, 1% Human serum albumin
Sequence info
Amino acid sequence
- T h e o r e t i c a l s e q u e n c e : A P P P N L P D P K F E S K A A L L A A R G P E E L L C F T E R L E D L V C F W E E A A S A G V G P G N Y S F S Y Q L E D E P W K L C R L H Q A P T A R G A V R F W C S L P T A D T S S F V P L E L R V T A A S G A P R Y H R V I H I N E V V L L D A P V G L V A R L A D E S G H V V L R W L P P P E T P M T S H I R Y E V D V S A G N G A G S V Q R V E I L E G R T E C V L S N L R G R T R Y T F A V R A R M A E P S F G G F W S A W S E P V S L L T P S D L D P R I P K V D K K V E P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C R V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K
- Accession
- P19235
- Protein length
- Fragment
- Amino acids
- 25 to 250
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation (PubMed:10388848, PubMed:2163695, PubMed:2163696, PubMed:8662939, PubMed:9774108). Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade (By similarity). In some cell types, can also activate STAT1 and STAT3 (PubMed:11756159). May also activate the LYN tyrosine kinase (By similarity).
- Sequence similarities
Belongs to the type I cytokine receptor family. Type 1 subfamily.
- Post-translational modifications
On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2 (PubMed:11781573). The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation (PubMed:10374881). Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11 (By similarity). Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site (PubMed:12027890).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle, Reconstitute for long term storage
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Previously labelled as EPO Receptor.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Erythropoietin receptor (EPO-R) also known as EPOR plays an important role in erythropoiesis by mediating the effects of erythropoietin (EPO). EPO-R is a member of the cytokine receptor family with a mass of approximately 55 kDa. This receptor can primarily be found on the surface of erythroid progenitor cells in the bone marrow. Its presence is important for the final differentiation of these cells into mature erythrocytes.
- Biological function summary
EPO-R functions by dimerizing upon EPO binding subsequently activating intracellular signaling pathways. It is a component of a larger signaling complex that recruits and activates various molecules initiating a cascade of events critical for cell survival and proliferation. This receptor signaling prevents apoptosis in erythroid progenitor cells promoting their growth and differentiation into red blood cells.
- Pathways
EPO-R's activity falls within the JAK-STAT and PI3K-AKT signaling pathways. These pathways are pivotal in regulating hematopoiesis and cell survival. JAK2 acts as a direct partner to EPO-R and transduces the signal downstream while molecules like STAT5 get phosphorylated and mediate transcription of target genes. The PI3K-AKT pathway also contributes to the anti-apoptotic effect essential for erythrocyte development.
- Associated diseases and disorders
Altered EPO-R signaling associates with anemia of chronic disease and polycythemia vera. Anemic conditions often show reduced sensitivity or expression of EPO-R while mutations leading to EPO-R hypersensitivity link to polycythemia vera. Dysregulation of related proteins such as JAK2 also contributes to these conditions indicating the interconnected roles of these proteins in blood-related diseases.
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1 product image
SDS-PAGE - Recombinant human EPO-R protein (Fc Chimera) (ab84001)
Lane 1 – MW markers; Lane 2 – ab84001 ; Lane 3 – ab84001 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab84001 treated with a glycosidase cocktail to remove potential N- and O linked glycans. 10 μg of protein was loaded per lane. Gel was stained with Deep Purple™.
Appearance of additional band at lower MW after treatment with PNGase F indicates the presence of N-linked glycans. A possible subsequent drop in MW after treatment with a glycosidase cocktail indicates O-linked glycans may be present. Additional high MW bands in lane 4 are glycosidase enzymes.
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