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AB162265

Recombinant Human Epsin 1 protein (GST tag N-Terminus)

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Recombinant Human Epsin 1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 550 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

Epsin-1, EH domain-binding mitotic phosphoprotein, EPS-15-interacting protein 1, EPN1

1 Images
SDS-PAGE - Recombinant Human Epsin 1 protein (GST tag N-Terminus) (AB162265)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Epsin 1 protein (GST tag N-Terminus) (AB162265)

ab162265 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q9Y6I3

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB75879

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RabMAb|Recombinant

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Proteins & Peptides

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Recombinant Mouse OSTM1 protein (His tag)

SDS-PAGE - Recombinant Mouse OSTM1 protein (His tag) (AB277009)

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Proteins & Peptides

AB276605

Recombinant Human SEZ6L2 protein (His tag)

SDS-PAGE - Recombinant Human SEZ6L2 protein (His tag) (AB276605)

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Proteins & Peptides

AB162126

Recombinant Human MMADHC protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human MMADHC protein (GST tag N-Terminus) (AB162126)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MSTSSLRRQMKNIVHNYSEAEIKVREATSNDPWGPSSSLMSEIADLTYNVVAFSEIMSMIWKRLNDHGKNWRHVYKAMTLMEYLIKTGSERVSQQCKENMYAVQTLKDFQYVDRDGKDQGVNVREKAKQLVALLRDEDRLREERAHALKTKEKLAQTATASSAAVGSGPPPEAEQAWPQSSGEEELQLQLALAMSKEEADQEERIRRGDDLRLQMAIEESKRETGGKEESSLMDLADVFTAPAPAPTTDPWGGPAPMAAAVPTAAPTSDPWGGPPVPPAADPWGGPAPTPASGDPWRPAAPAGPSVDPWGGTPAPAAGEGPTPDPWGSSDGGVPVSGPSASDPWTPAPAFSDPWGGSPAKPSTNGTTAGGFDTEPDEFSDFDRLRTALPTSGSSAGELELLAGEVPARSPGAFDMSGVRGSLAEAVGSPPPAATPTPTPPTRKTPESFLGPNAALVDLDSLVSRPGPTPPGAKASNPFLPGGGPATGPSVTNPFQPAPPATLTLNQLRLSPVPPVPGAPPTYISPLGGGPGLPPMMPPGPPAPNTNPFLL","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":550,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9Y6I3","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epsin 1 commonly referred to as EPN1 is a protein that primarily participates in the process of endocytosis. It exhibits a molecular mass of approximately 68 kDa. Epsin 1 is found mainly in the cytoplasm but also shows a high expression in the brain indicating its importance in neurological functions. Additionally alternative splicing can lead to different isoforms enriching its functional diversity.
Biological function summary

Epsin 1 works as an endocytic accessory protein that plays a role in clathrin-mediated endocytosis facilitating the internalization of various receptors and membrane proteins. It interacts with clathrin and AP-2 adaptor complex helping to deform the membrane to form vesicles. Epsin 1 is known to bind phospholipids via its ENTH domain which is important for its function at the plasma membrane. This protein does not work alone and is part of larger protein complexes that include other proteins involved in vesicle formation and maturation.

Pathways

Epsin 1 participates in the endocytic pathway essential for the regulation of nutrient uptake and signaling receptor modulation. It coordinates with proteins such as dynamin and adaptin ensuring the proper invagination of the membrane and vesicle scission. Epsin 1 is also linked to the actin cytoskeleton affecting pathways linked to signal transduction and cellular organization.

Epsin 1 has been implicated in cancer and neurological disorders. Abnormal expression levels of Epsin 1 have been associated with tumor progression and metastasis particularly in breast cancer. This correlation suggests that Epsin 1 may influence pathways with epidermal growth factor receptor (EGFR) known for its role in cancer cell proliferation. Moreover its prominent expression in neurons indicates a possible connection to neurodegenerative diseases where similar proteins like amyloid precursor protein (APP) are involved raising interest in its potential role in Alzheimer's disease.
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Specifications

Form

Liquid

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General info

Function

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis (PubMed : 10393179, PubMed : 10557078).

Sequence similarities

Belongs to the epsin family.

Post-translational modifications

Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.. Ubiquitinated.

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Product protocols

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Target data

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis (PubMed : 10393179, PubMed : 10557078).
See full target information Epsin-1
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Product promise

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