Recombinant human ErbB2 / HER2 (mutated G776V + G776C) protein (Cytoplasmic domain) is a Human Fragment protein, in the 676 to 1255 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >75% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
K R R Q Q K I R K Y T M R R L L Q E T E L V E P L T P S G A M P N Q A Q M R I L K E T E L R K V K V L G S G A F G T V Y K G I W I P D G E N V K I P V A I K V L R E N T S P K A N K E I L D E A Y V M A G V G S P Y V S R L L G I C L T S T V Q L V T Q L M P Y G C L L D H V R E N R G R L G S Q D L L N W C M Q I A K G M S Y L E D V R L V H R D L A A R N V L V K S P N H V K I T D F G L A R L L D I D E T E Y H A D G G K V P I K W M A L E S I L R R R F T H Q S D V W S Y G V T V W E L M T F G A K P Y D G I P A R E I P D L L E K G E R L P Q P P I C T I D V Y M I M V K C W M I D S E C R P R F R E L V S E F S R M A R D P Q R F V V I Q N E D L G P A S P L D S T F Y R S L L E D D D M G D L V D A E E Y L V P Q Q G F F C P D P A P G A G G M V H H R H R S S S T R S G G G D L T L G L E P S E E E A P R S P L A P S E G A G S D V F D G D L G M G A A K G L Q S L P T H D P S P L Q R Y S E D P T V P L P S E T D G Y V A P L T C S P Q P E Y V N Q P D V R P Q P P S P R E G P L P A A R P A G A T L E R P K T L S P G K N G V V K D V F A F G G A V E N P E Y L T P Q G G A A P Q P H P P P A F S P A F D N L Y Y W D Q D P P E R G A P P S T F K G T P T A E N P E Y L G L D V P V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
Alternative names
CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19
Recommended products
Recombinant human ErbB2 / HER2 (mutated G776V + G776C) protein (Cytoplasmic domain) is a Human Fragment protein, in the 676 to 1255 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >75% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity: 5 nmol/min/mg.
- Accession
- P04626-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- K R R Q Q K I R K Y T M R R L L Q E T E L V E P L T P S G A M P N Q A Q M R I L K E T E L R K V K V L G S G A F G T V Y K G I W I P D G E N V K I P V A I K V L R E N T S P K A N K E I L D E A Y V M A G V G S P Y V S R L L G I C L T S T V Q L V T Q L M P Y G C L L D H V R E N R G R L G S Q D L L N W C M Q I A K G M S Y L E D V R L V H R D L A A R N V L V K S P N H V K I T D F G L A R L L D I D E T E Y H A D G G K V P I K W M A L E S I L R R R F T H Q S D V W S Y G V T V W E L M T F G A K P Y D G I P A R E I P D L L E K G E R L P Q P P I C T I D V Y M I M V K C W M I D S E C R P R F R E L V S E F S R M A R D P Q R F V V I Q N E D L G P A S P L D S T F Y R S L L E D D D M G D L V D A E E Y L V P Q Q G F F C P D P A P G A G G M V H H R H R S S S T R S G G G D L T L G L E P S E E E A P R S P L A P S E G A G S D V F D G D L G M G A A K G L Q S L P T H D P S P L Q R Y S E D P T V P L P S E T D G Y V A P L T C S P Q P E Y V N Q P D V R P Q P P S P R E G P L P A A R P A G A T L E R P K T L S P G K N G V V K D V F A F G G A V E N P E Y L T P Q G G A A P Q P H P P P A F S P A F D N L Y Y W D Q D P P E R G A P P S T F K G T P T A E N P E Y L G L D V P V
- Accession
- P04626
- Protein length
- Fragment
- Predicted molecular weight
- 115 kDa
- Amino acids
- 676 to 1255
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
- Post-translational modifications
Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172, PubMed:33497358). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).
- Subcellular localisation
- Early endosome, Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.
- Biological function summary
The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.
- Pathways
ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.
- Associated diseases and disorders
ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.
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4 product images
Functional Studies - Recombinant human ErbB2 / HER2 (mutated G776V + G776C) protein (Cytoplasmic domain) (ab191466)
The specific activity of ErbB2 / HER2 (ab191466) was determined to be 175 nmol/min/mg as per activity assay protocol and was equivalent to 6 nmol/min/mg as per radiometric assay
SDS-PAGE - Recombinant human ErbB2 / HER2 (mutated G776V + G776C) protein (Cytoplasmic domain) (ab191466)
SDS PAGE analysis of ab191466
SDS-PAGE - Recombinant human ErbB2 / HER2 (mutated G776V + G776C) protein (Cytoplasmic domain) (ab191466)
SDS-PAGE of ErbB 2 (mutated G776 VC). Approx MW 115 kDa.
Functional Studies - Recombinant human ErbB2 / HER2 (mutated G776V + G776C) protein (Cytoplasmic domain) (ab191466)
Specific Activity: Sample Kinase Activity Plot. ErbB 2 (mutated G776 VC) activity assay with or without substrate Poly (Glu:Tyr, 4:1) peptide. The specific activity of ErbB2 / HER2 (mutated G776 VC) was determined to be 5 nmol /min/mg.
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