Recombinant Human ERK2 protein

• WB

Lab

Western blot - Recombinant Human ERK2 protein (AB43625)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205819 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-ERK2 antibody [E460] (<a href='/en-us/products/primary-antibodies/hrp-erk2-antibody-e460-ab205819'>ab205819</a>) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (<a href='/en-us/products/cell-lysates/hela-whole-cell-lysate-ab150035'>ab150035</a>) at 10 µg

Lane 2:

46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate at 10 µg

Lane 3:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Recombinant Human ERK1 protein (<a href='/en-us/products/unavailable/recombinant-human-erk1-protein-ab43623-ab43623'>ab43623</a>) (<a href='/en-us/products/unavailable/recombinant-human-erk1-protein-ab43623-ab43623'>ab43623</a>) at 0.1 µg

Lane 5:

Western blot - Recombinant Human ERK2 protein (ab43625) at 0.1 µg

Lane 6:

Wild-type HAP1 cell lysate at 20 µg

Lane 7:

ERK2 knockout HAP1 cell lysate at 20 µg

Predicted band size: 41 kDa

Observed band size: 41 kDa

true

Exposure time: 12min

• WB

Lab

Western blot - Recombinant Human ERK2 protein (AB43625)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab32081 overnight at 4°C. Antibody binding was detected using ab175781 (goat anti-rabbit Alexa Fluor 790) at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-ERK2 antibody [E460] (<a href='/en-us/products/primary-antibodies/erk2-antibody-e460-ab32081'>ab32081</a>) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Lane 3:

46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate at 20 µg

Lane 4:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg

Lane 5:

Recombinant Human ERK1 protein (<a href='/en-us/products/unavailable/recombinant-human-erk1-protein-ab43623-ab43623'>ab43623</a>) (<a href='/en-us/products/unavailable/recombinant-human-erk1-protein-ab43623-ab43623'>ab43623</a>) at 20 µg

Lane 6:

Western blot - Recombinant Human ERK2 protein (ab43625) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-alexa-fluor-790-ab175781'>ab175781</a>) at 1/10000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

• WB

Lab

Western blot - Recombinant Human ERK2 protein (AB43625)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab32537 overnight at 4°C. Antibody binding was detected using a goat anti-rabbit Alexa Fluor® 790) ab175781 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-ERK1 antibody [Y72] (<a href='/en-us/products/primary-antibodies/erk1-antibody-y72-ab32537'>ab32537</a>) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Lane 3:

Recombinant Human ERK1 protein (<a href='/en-us/products/unavailable/recombinant-human-erk1-protein-ab43623-ab43623'>ab43623</a>) (<a href='/en-us/products/unavailable/recombinant-human-erk1-protein-ab43623-ab43623'>ab43623</a>) at 20 µg

Lane 4:

Western blot - Recombinant Human ERK2 protein (ab43625) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-alexa-fluor-790-ab175781'>ab175781</a>) at 1/10000 dilution

Predicted band size: 43 kDa

Observed band size: 44 kDa

false

• WB

Supplier Data

Western blot - Recombinant Human ERK2 protein (AB43625)

Recombinant full length ERK2 protein (ab43625) contains aa1-360. Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (<a href='/en-us/products/primary-antibodies/erk1-erk2-antibody-epr17526-ab184699'>ab184699</a>) at 1/10000 dilution

All lanes:

Western blot - Recombinant Human ERK2 protein (ab43625) at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa,52 kDa

Observed band size: 42 kDa

false

• SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ERK2 protein (AB43625)

The purity of ab43625 was determined to be >90% by SDS-PAGE. MW ~42kDa.