Recombinant human ERK5 protein is a Human Full Length protein, in the 1 to 816 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, WB, FuncS.
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- Current issues in molecular biology 45:6154-61692023Identification of a Novel ERK5 (MAPK7) Inhibitor, MHJ-627, and Verification of Its Potent Anticancer Efficacy in Cervical Cancer HeLa Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeonghye Hwang,Hyejin Moon,Hakwon Kim,Ki-Young KimPubMed 37504304
- Nature communications 8:4942017Metabolic stress-induced cardiomyopathy is caused by mitochondrial dysfunction due to attenuated Erk5 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Liu et. al.PubMed 28887535
Key facts
- Purity
>75% Densitometry
- Expression system
Baculovirus infected Sf9 cells
- Tags
Tag free
- Applications
SDS-PAGE, WB, FuncS
- Biologically active
Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.
Alternative names
Mitogen-activated protein kinase 7, MAP kinase 7, MAPK 7, Big MAP kinase 1, Extracellular signal-regulated kinase 5, BMK-1, ERK-5, MAPK7, BMK1, ERK5, PRKM7
Recommended products
Recombinant human ERK5 protein is a Human Full Length protein, in the 1 to 816 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
>75% Densitometry
- Expression system
Baculovirus infected Sf9 cells
- Tags
Tag free
- Applications
SDS-PAGE, WB, FuncS
- Biologically active
Yes
- Biological activity
The specific activity of ab126913 was determined to be 11 nmol/min/mg
- Accession
- Q13164-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- Accession
- Protein length
Full Length
- Predicted molecular weight
132 kDa
- Amino acids
1 to 816
- Nature
Recombinant
Specifications
- Form
Liquid
- Additional notes
Purity was determined to be >75% by densitometry. Affinity purified.
General info
- Function
Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.
- Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
- Post-translational modifications
Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
- Subcellular localisation
Nucleus, PML body
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Native Pig Myelin Basic Protein ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ERK5 also known as big MAP kinase 1 (BMK1) is an enzyme that functions as a protein-serine/threonine kinase within the MAP kinase family. It has a molecular weight of approximately 120 kDa. ERK5 is expressed in various tissues including the brain heart and lungs with notable expression in endothelial cells. The protein plays a mechanical role in signal transduction processes which it achieves by transmitting signals from extracellular sources to the cellular nucleus facilitating the regulation of gene expression.
- Biological function summary
ERK5 influences cellular functions through its role in promoting cell proliferation and differentiation. It serves as part of a signaling complex that includes the MAP kinase kinase 5 (MEK5). This interaction allows ERK5 to phosphorylate and subsequently activate target substrates such as transcription factors promoting cellular responses necessary for development and survival. ERK5 also plays a role in the response to oxidative stress which contributes to its impact on survival pathways and cellular stress responses.
- Pathways
ERK5 acts within the MAPK signaling cascade and is linked to the ERK/MAPK pathway and the phosphoinositide 3-kinase (PI3K) pathway. Within these pathways ERK5 interacts with other MAP kinases and regulatory proteins such as Ras and Raf contributing to a wide range of cellular processes including growth migration and survival. Through these interactions ERK5 influences cellular dynamics and mediates responses to growth factors and stress stimuli.
- Associated diseases and disorders
Abnormal activity of ERK5 has been implicated in various conditions including cardiovascular disease and cancer. Elevated ERK5 signaling can promote tumor progression and metastasis in certain cancers by enhancing tumor cell proliferation and survival. In cardiovascular disorders ERK5 interacts with proteins like calcium/calmodulin-dependent protein kinase II (CaMKII) influencing pathological cardiac remodeling and hypertrophy. These associations highlight ERK5's impact on disease mechanisms and highlight its potential as a therapeutic target.
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4 product images
Functional Studies - Recombinant human ERK5 protein (ab126913)
The specific activity of ERK5 (ab126913) was determined to be 2.5 nmol/min/mg as per activity assay protocol and was equivalent to 12.7 nmol/min/mg as per radiometric assay
SDS-PAGE - Recombinant human ERK5 protein (ab126913)
SDS PAGE analysis of ab126913
SDS-PAGE - Recombinant human ERK5 protein (ab126913)
SDS-PAGE using ab126913
Functional Studies - Recombinant human ERK5 protein (ab126913)
The specific activity of ab126913 was determined to be 11 nmol/min/mg
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