Recombinant Human Erlin-2 protein (denatured) is a Human Fragment protein, in the 25 to 339 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S K I E E G H I G V Y Y R G G A L L T S T S G P G F H L M L P F I T S Y K S V Q T T L Q T D E V K N V P C G T S G G V M I Y F D R I E V V N F L V P N A V Y D I V K N Y T A D Y D K A L I F N K I H H E L N Q F C S V H T L Q E V Y I E L F D Q I D E N L K L A L Q Q D L T S M A P G L V I Q A V R V T K P N I P E A I R R N Y E L M E S E K T K L L I A A Q K Q K V V E K E A E T E R K K A L I E A E K V A Q V A E I T Y G Q K V M E K E T E K K I S E I E D A A F L A R E K A K A D A E C Y T A M K I A E A N K L K L T P E Y L Q L M K Y K A I A S N S K I Y F G K D I P N M F M D S A G S V S K Q F E G L A D K L S F G L E D E P L E T A T K E N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs) such as ITPR1 (PubMed:17502376, PubMed:19240031). Promotes sterol-accelerated ERAD of HMGCR probably implicating an AMFR/gp78-containing ubiquitin ligase complex (PubMed:21343306). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. May promote ER retention of the SCAP-SREBF complex (PubMed:24217618).
Alternative names
C8orf2, SPFH2, UNQ2441/PRO5003/PRO9924, ERLIN2, Erlin-2, Endoplasmic reticulum lipid raft-associated protein 2, Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2, SPFH domain-containing protein 2
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Recombinant Human Erlin-2 protein (denatured) is a Human Fragment protein, in the 25 to 339 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O94905-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 12.01% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S K I E E G H I G V Y Y R G G A L L T S T S G P G F H L M L P F I T S Y K S V Q T T L Q T D E V K N V P C G T S G G V M I Y F D R I E V V N F L V P N A V Y D I V K N Y T A D Y D K A L I F N K I H H E L N Q F C S V H T L Q E V Y I E L F D Q I D E N L K L A L Q Q D L T S M A P G L V I Q A V R V T K P N I P E A I R R N Y E L M E S E K T K L L I A A Q K Q K V V E K E A E T E R K K A L I E A E K V A Q V A E I T Y G Q K V M E K E T E K K I S E I E D A A F L A R E K A K A D A E C Y T A M K I A E A N K L K L T P E Y L Q L M K Y K A I A S N S K I Y F G K D I P N M F M D S A G S V S K Q F E G L A D K L S F G L E D E P L E T A T K E N
- Accession
- O94905
- Protein length
- Fragment
- Predicted molecular weight
- 37.8 kDa
- Amino acids
- 25 to 339
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs) such as ITPR1 (PubMed:17502376, PubMed:19240031). Promotes sterol-accelerated ERAD of HMGCR probably implicating an AMFR/gp78-containing ubiquitin ligase complex (PubMed:21343306). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. May promote ER retention of the SCAP-SREBF complex (PubMed:24217618).
- Sequence similarities
Belongs to the band 7/mec-2 family.
- Post-translational modifications
Deubiquitinated by USP25; leading to stabilization.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as SPFH2.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Erlin-2 also known as SPFH domain family member 2 is a protein with an approximate mass of 40 kDa. The protein is expressed in the endoplasmic reticulum where it participates in various cellular processes. Erlin-2 shares similarities structural with its homolog Erlin-1. Erlin-2 is part of a larger family of erlins that play essential roles in cellular regulation.
- Biological function summary
The functions of Erlin-2 involve its participation as a member of the erlins complex which includes Erlin-1 and other proteins. This complex helps regulate the degradation of inositol 145-trisphosphate receptors. Furthermore Erlin-2 facilitates the process of lipid raft-associated protein transport and maintains lipid homeostasis inside cells.
- Pathways
Erlin-2 contributes to the endoplasmic reticulum-associated degradation pathway which is important for managing protein quality control. In coordination with Erlin-1 Erlin-2 influences calcium signaling pathways by regulating the turnover of inositol 145-trisphosphate receptors. These pathways play a significant role in maintaining cellular homeostasis and responding to stress conditions.
- Associated diseases and disorders
Researchers have linked Erlin-2 to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS). Abnormalities in calcium signaling pathways mediated by Erlin-2 and associated with inositol 145-trisphosphate receptors can contribute to neuronal dysfunction in these diseases. Additionally changes in Erlin-2 expression may relate to certain cancers suggesting its potential role in tumorigenesis.
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1 product image
SDS-PAGE - Recombinant Human Erlin-2 protein (denatured) (ab136707)
15% SDS-PAGE analysis of 3 µg ab136707.
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