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AB101664

Recombinant Human ERP27 protein

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Recombinant Human ERP27 protein is a Human Full Length protein, in the 26 to 273 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

C12orf46, UNQ781/PRO1575, ERP27, Endoplasmic reticulum resident protein 27, ER protein 27, ERp27, Inactive protein disulfide-isomerase 27

1 Images
SDS-PAGE - Recombinant Human ERP27 protein (AB101664)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ERP27 protein (AB101664)

ab101664 at 3 ug on an SDS-PAGE gel (15%)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q96DN0

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.242% Tris, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMEVEKSSDGPGAAQEPTWLTDVPAAMEFIAATEVAVIGFFQDLEIPAVPILHSMVQKFPGVSFGISTDSEVLTHYNITGNTICLFRLVDNEQLNLEDEDIESIDATKLSRFIEINSLHMVTEYNPVTVIGLFNSVIQIHLLLIMNKASPEYEENMHRYQKAAKLFQGKILFILVDSGMKENGKVISFFKLKESQLPALAIYQTLDDEWDTLPTAEVSVEHVQNFCDGFLSGKLLKENRESEGKTPKVEL","proteinLength":"Full Length","predictedMolecularWeight":"30 kDa","actualMolecularWeight":null,"aminoAcidEnd":273,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96DN0","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERP27 also known as Endoplasmic Reticulum Protein 27 is a protein component with a molecular mass of approximately 27 kDa. It resides within the endoplasmic reticulum (ER) of the cell where it participates in protein folding and processing. Expressed in many tissues ERP27 is part of the protein disulfide isomerase (PDI) family. Although its structural features do not include the typical thioredoxin-like active sites ERP27 influences protein maturation by assisting in the correct assembly and folding of nascent proteins.
Biological function summary

The protein exhibits important roles in the cellular stress response and quality control mechanisms within the endoplasmic reticulum. It does not form a stable complex with other proteins but interacts transiently to exert its function. ERP27 collaborates closely with other ER-resident proteins like ERP44 and the PDI family to enhance the checking and refolding of misfolded proteins maintaining cellular homeostasis.

Pathways

ERP27 participates directly in the unfolded protein response (UPR) pathway a critical mechanism activated during ER stress. This pathway involves ERP27's transient interactions within a network of proteins including ER chaperones and other PDIs to alleviate stress and restore normal function. Furthermore ERP27 plays a role in calcium homeostasis through these pathways interacting with proteins such as calnexin and calreticulin.

ERP27's impairment or dysregulation associates with neurodegenerative diseases like Alzheimer's and Parkinson’s. The protein's involvement in the UPR links it to cellular processes that are disrupted in these conditions. Moreover it connects to proteins such as amyloid precursor protein (APP) in Alzheimer's influencing amyloid-beta peptide production. In Parkinson’s interactions with parkin and related proteins highlight ERP27's potential implications in disease pathogenesis and progression.
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Specifications

Form

Liquid

Additional notes

purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

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General info

Function

Specifically binds unfolded proteins and may recruit protein disulfide isomerase PDIA3 to unfolded substrates (PubMed : 16940051, PubMed : 23192347). Binds protein substrates via a hydrophobic pocket in the C-terminal domain (PubMed : 16940051, PubMed : 23192347). May play a role in the unfolded stress response (PubMed : 23192347).

Sequence similarities

Belongs to the protein disulfide isomerase family.

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Product protocols

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Target data

Specifically binds unfolded proteins and may recruit protein disulfide isomerase PDIA3 to unfolded substrates (PubMed : 16940051, PubMed : 23192347). Binds protein substrates via a hydrophobic pocket in the C-terminal domain (PubMed : 16940051, PubMed : 23192347). May play a role in the unfolded stress response (PubMed : 23192347).
See full target information ERP27
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