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AB92937

Recombinant Human ERp57 protein (His tag N-Terminus)

5

(1 Review)

|

(3 Publications)

Recombinant Human ERp57 protein (His tag N-Terminus) is a Human Full Length protein, in the 25 to 505 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

ERP57, ERP60, GRP58, PDIA3, Protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, Endoplasmic reticulum resident protein 60, p58, ER protein 57, ERp57, ER protein 60, ERp60

3 Images
Western blot - Recombinant Human ERp57 protein (His tag N-Terminus) (AB92937)
  • WB

Unknown

Western blot - Recombinant Human ERp57 protein (His tag N-Terminus) (AB92937)

All lanes:

Western blot - Anti-ERp57 antibody (<a href='/en-us/products/primary-antibodies/erp57-antibody-ab10287'>ab10287</a>) at 1/1000 dilution

All lanes:

Western blot - Recombinant Human ERp57 protein (His tag N-Terminus) (ab92937) at 0.01 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution

Predicted band size: 57 kDa

true

Exposure time: 150s

Western blot - Recombinant Human ERp57 protein (His tag N-Terminus) (AB92937)
  • WB

Unknown

Western blot - Recombinant Human ERp57 protein (His tag N-Terminus) (AB92937)

All lanes:

Western blot - Anti-ERp57 antibody (<a href='/en-us/products/primary-antibodies/erp57-antibody-ab10287'>ab10287</a>) at 1/1000 dilution

All lanes:

Western blot - Recombinant Human ERp57 protein (His tag N-Terminus) (ab92937) at 0.01 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution

Predicted band size: 57 kDa

true

Exposure time: 150s

SDS-PAGE - Recombinant Human ERp57 protein (His tag N-Terminus) (AB92937)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ERp57 protein (His tag N-Terminus) (AB92937)

15% SDS-PAGE showing ab92937 (3μg) at approximately 58.5kDa.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

P30101

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab92937 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/erp57-antibody-ab10287'>ab10287</a>.</p>" } } }
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Sequence info

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMSDVLELTDDNFESRISDTGSAGLMLVEFFAPWCGHCKRLAPEYEAAATRLKGIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFFDDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDKTVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL","proteinLength":"Full Length","predictedMolecularWeight":"58.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":505,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P30101","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERp57 also known as 4f9 is a protein responsible for catalyzing disulfide bond formations and isomerizations in the endoplasmic reticulum. ERp57 has a molecular mass of approximately 57 kDa and belongs to the protein disulfide isomerase (PDI) family. Its main expression occurs in the endoplasmic reticulum of cells where it plays a role in ensuring proper protein folding and quality control.
Biological function summary

ERp57 interacts with calnexin and calreticulin to form a complex involved in glycoprotein folding. This complex assists in the proper assembly and maturation of nascent glycoproteins by ensuring correct folding pathways within the cell. ERp57 has a specialized role in managing oxidative protein folding which makes it essential for cell function and homeostasis.

Pathways

ERp57 participates in the calnexin/calreticulin cycle which is critical for protein triage in the endoplasmic reticulum. This cycle ensures that only properly folded proteins progress to their final destinations. ERp57 also interacts with ERp72 another member of the PDI family to facilitate thiol-disulfide exchange reactions. These interactions position ERp57 as a central player in the maintenance of cellular redox balance and protein homeostasis.

Alterations in ERp57 function can relate to neurodegenerative diseases such as Alzheimer's and certain types of cancer. Misfolded proteins often accumulate in these conditions highlighting ERp57's role in preventing cellular stress. Moreover ERp57 associates with the protein tau in Alzheimer's disease where it assists in tau’s proper folding and diminishes its aggregation tendency. Additionally disruptions in the ERp57 pathway may interact with cancer-related proteins like p53 modulating apoptosis and cell cycle regulation.
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Specifications

Form

Liquid

Additional notes

ab92937 is purified using conventional chromatography techniques.

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General info

Function

Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone (PubMed : 11825568, PubMed : 16193070, PubMed : 27897272, PubMed : 36104323, PubMed : 7487104). Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity (PubMed : 35948544, PubMed : 36104323).

Sequence similarities

Belongs to the protein disulfide isomerase family.

Post-translational modifications

Within the major histocompatibility complex class I (MHC I) peptide loading complex forms reversible disulfide-linked heterodimers with TAPBP as part of its protein folding chaperone activity. This is essential to assist the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum.. Phosphorylated.

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Product protocols

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Target data

Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone (PubMed : 11825568, PubMed : 16193070, PubMed : 27897272, PubMed : 36104323, PubMed : 7487104). Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity (PubMed : 35948544, PubMed : 36104323).
See full target information PDIA3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 7: PubMed36509292

2022

Single-cell analysis of senescent epithelia reveals targetable mechanisms promoting fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Eoin D O'Sullivan,Katie J Mylonas,Rachel Bell,Cyril Carvalho,David P Baird,Carolynn Cairns,Kevin M Gallagher,Ross Campbell,Marie Docherty,Alexander Laird,Neil C Henderson,Tamir Chandra,Kristina Kirschner,Bryan Conway,Gry H Dihazi,Michael Zeisberg,Jeremy Hughes,Laura Denby,Hassan Dihazi,David A Ferenbach

American journal of physiology. Cell physiology 322:C624-C644 PubMed35196163

2022

PDIA3/ERp57 promotes a matrix-rich secretome that stimulates fibroblast adhesion through CCN2.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew L Hellewell,Kate J Heesom,Mark A Jepson,Josephine C Adams

Bioscience reports 40: PubMed33095243

2020

Impairment of cell adhesion and migration by inhibition of protein disulphide isomerases in three breast cancer cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Henry S Young,Lucy M McGowan,Katy A Jepson,Josephine C Adams
View all publications
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Complementary Products

Primary Antibodies

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Anti-PCNA antibody [PC10]

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PCNA antibody [PC10] (AB29)

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