Recombinant Human ERp72 protein (His tag) is a Human Fragment protein, in the 1 to 641 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M R P R K A F L L L L L L G L V Q L L A V A G A E G P D E D S S N R E N A I E D E E E E E E E D D D E E E D D L E V K E E N G V L V L N D A N F D N F V A D K D T V L L E F Y A P W C G H C K Q F A P E Y E K I A N I L K D K D P P I P V A K I D A T S A S V L A S R F D V S G Y P T I K I L K K G Q A V D Y E G S R T Q E E I V A K V R E V S Q P D W T P P P E V T L V L T K E N F D E V V N D A D I I L V E F Y A P W C G H C K K L A P E Y E K A A K E L S K R S P P I P L A K V D A T A E T D L A K R F D V S G Y P T L K I F R K G R P Y D Y N G P R E K Y G I V D Y M I E Q S G P P S K E I L T L K Q V Q E F L K D G D D V I I I G V F K G E S D P A Y Q Q Y Q D A A N N L R E D Y K F H H T F S T E I A K F L K V S Q G Q L V V M Q P E K F Q S K Y E P R S H M M D V Q G S T Q D S A I K D F V L K Y A L P L V G H R K V S N D A K R Y T R R P L V V V Y Y S V D F S F D Y R A A T Q F W R S K V L E V A K D F P E Y T F A I A D E E D Y A G E V K D L G L S E S G E D V N A A I L D E S G K K F A M E P E E F D S D T L R E F V T A F K K G K L K P V I K S Q P V P K N N K G P V K V V V G K T F D S I V M D P K K D V L I E F Y A P W C G H C K Q L E P V Y N S L A K K Y K G Q K G L V I A K M D A T A N D V P S D R Y K V E G F P T I Y F A P S G D K K N P V K F E G G D R D L E H L S K F I E E H A T K L S R T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
ERP70, ERP72, PDIA4, Protein disulfide-isomerase A4, Endoplasmic reticulum resident protein 70, Endoplasmic reticulum resident protein 72, ER protein 70, ERp70, ER protein 72, ERp-72, ERp72
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Recombinant Human ERp72 protein (His tag) is a Human Fragment protein, in the 1 to 641 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P13667-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M R P R K A F L L L L L L G L V Q L L A V A G A E G P D E D S S N R E N A I E D E E E E E E E D D D E E E D D L E V K E E N G V L V L N D A N F D N F V A D K D T V L L E F Y A P W C G H C K Q F A P E Y E K I A N I L K D K D P P I P V A K I D A T S A S V L A S R F D V S G Y P T I K I L K K G Q A V D Y E G S R T Q E E I V A K V R E V S Q P D W T P P P E V T L V L T K E N F D E V V N D A D I I L V E F Y A P W C G H C K K L A P E Y E K A A K E L S K R S P P I P L A K V D A T A E T D L A K R F D V S G Y P T L K I F R K G R P Y D Y N G P R E K Y G I V D Y M I E Q S G P P S K E I L T L K Q V Q E F L K D G D D V I I I G V F K G E S D P A Y Q Q Y Q D A A N N L R E D Y K F H H T F S T E I A K F L K V S Q G Q L V V M Q P E K F Q S K Y E P R S H M M D V Q G S T Q D S A I K D F V L K Y A L P L V G H R K V S N D A K R Y T R R P L V V V Y Y S V D F S F D Y R A A T Q F W R S K V L E V A K D F P E Y T F A I A D E E D Y A G E V K D L G L S E S G E D V N A A I L D E S G K K F A M E P E E F D S D T L R E F V T A F K K G K L K P V I K S Q P V P K N N K G P V K V V V G K T F D S I V M D P K K D V L I E F Y A P W C G H C K Q L E P V Y N S L A K K Y K G Q K G L V I A K M D A T A N D V P S D R Y K V E G F P T I Y F A P S G D K K N P V K F E G G D R D L E H L S K F I E E H A T K L S R T
- Accession
- P13667
- Protein length
- Fragment
- Predicted molecular weight
- 71.6 kDa
- Amino acids
- 1 to 641
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Sequence similarities
Belongs to the protein disulfide isomerase family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ERp72 also known as PDIA4 is a protein with a molecular weight of approximately 72 kDa. It belongs to the protein disulfide isomerase (PDI) family and functions as an enzyme within the endoplasmic reticulum (ER). The protein helps with folding by catalyzing the formation rearrangement and breakage of disulfide bonds in nascent proteins. ERp72 is expressed in a variety of tissues with high abundance in the liver pancreas and other secretory organs reflecting its role in protein synthesis and processing.
- Biological function summary
The protein disulfide isomerase family member ERp72 plays a significant part in maintaining ER function. It participates in the unfolded protein response (UPR) which ensures proper protein folding and manages ER stress. ERp72 operates as part of a larger multiprotein complex that aids in the quality control of newly synthesized proteins. The protein interacts with calnexin and BiP to perform these functions. This makes ERp72 essential for cellular homeostasis and adaptation to changing physiological conditions.
- Pathways
ERp72 is closely involved in the protein folding and ER stress response pathways. The unfolded protein response pathway recognizes misfolded proteins in the ER and initiates mechanisms to restore normal function. It connects with other proteins such as ATF6 and IRE1 to modulate gene expression and improve protein folding capacity. Additionally ERp72 participates in the pathway related to oxidative protein folding working alongside proteins like ERO1. This confirms its key contribution to cellular function by maintaining the balance between protein folding and degradation.
- Associated diseases and disorders
Alterations in ERp72 expression or function link to conditions such as neurodegenerative diseases and cancer. Alzheimer's disease shows aberrant ER stress and misfolded protein accumulation where ERp72's interaction with amyloid precursor protein might play a role. In the case of cancer ER stress and UPR pathway alterations contribute to cancer cell survival associating ERp72 with this adaptation process particularly its interaction with BiP and other PDIs. These connections highlight the relevance of ERp72 in disease progression and as a potential therapeutic target.
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SDS-PAGE - Recombinant Human ERp72 protein (His tag) (ab276531)
SDS-PAGE analysis of ab276531
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