Recombinant Human Estrogen Receptor beta protein is a Human Fragment protein, in the 22 to 121 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
I L P L E H G S I Y I P S S Y V D S H H E Y P A M T F Y S P A V M N Y S I P S N V T N L E G G P G R Q T T S P N V L W P T P G H L S P L V V H R Q L S H L Y A E P Q K S P W C E A R S L E H T L P V N R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes (Recombinant protein) |
Target data
Function
Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). Isoform 2. Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.
Alternative names
ESTRB, NR3A2, ESR2, Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2
Recommended products
Recombinant Human Estrogen Receptor beta protein is a Human Fragment protein, in the 22 to 121 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q92731-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- I L P L E H G S I Y I P S S Y V D S H H E Y P A M T F Y S P A V M N Y S I P S N V T N L E G G P G R Q T T S P N V L W P T P G H L S P L V V H R Q L S H L Y A E P Q K S P W C E A R S L E H T L P V N R
- Accession
- Q92731
- Protein length
- Fragment
- Predicted molecular weight
- 36.63 kDa
- Amino acids
- 22 to 121
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560).
- Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
- Post-translational modifications
Phosphorylation at Ser-87 and Ser-105 recruits NCOA1.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Estrogen Receptor beta (ERβ) also known as ERbeta or beta receptor is an important nuclear receptor involved in mediating the effects of estrogens such as estradiol. This receptor with a molecular weight of approximately 59 kDa resides mainly in endothelial and epithelial tissues and can also be present in the central nervous system. ERβ possesses the ability to bind ligands and translocate to the nucleus where it regulates the transcription of specific target genes.
- Biological function summary
Estrogen Receptor beta plays a significant role in cell growth regulation and differentiation. It often forms dimers with Estrogen Receptor alpha (ERα) contributing to the diversity of estrogen signaling responses. As part of the nuclear receptor family ERβ engages in binding with DNA at estrogen response elements influencing various physiological processes. The presence of ERβ in tissues implies it has a potentially protective role possibly balancing the actions of ERα.
- Pathways
Estrogen Receptor beta plays an essential role in the estrogen signaling pathway and the apoptosis pathway. Interaction with ERα can influence pathways related to cell proliferation and survival contributing to distinct physiological outcomes. ERβ's involvement with proteins like the retinoic acid receptor within these pathways highlights its impact on controlling gene expression important for cell cycle regulation and programmed cell death.
- Associated diseases and disorders
Estrogen Receptor beta has implications in cancers and cardiovascular diseases. In breast cancer ERβ may act differently from ERα possibly providing protective effects against tumor growth. Moreover the receptor's expression affects cardiovascular health by modulating inflammation and vascular function. ERβ interacts with proteins such as p53 hinting at its role in controlling cellular stress responses and combating oncogenesis.
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1 product image
SDS-PAGE - Recombinant Human Estrogen Receptor beta protein (ab114181)
ab114181 on a 12.5% SDS-PAGE Stained with Coomassie Blue.
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Product protocols
- Visit the general protocols
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