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AB107152

Recombinant Human ETFB protein

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Recombinant Human ETFB protein is a Human Full Length protein, in the 1 to 255 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

FP585, ETFB, Electron transfer flavoprotein subunit beta, Beta-ETF

1 Images
SDS-PAGE - Recombinant Human ETFB protein (AB107152)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ETFB protein (AB107152)

15% SDS-PAGE showing ab107152 (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P38117

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAELRVLVAVKRVIDYAVKIRVKPDRTGVVTDGVKHSMNPFCEIAVEEAVRLKEKKLVKEVIAVSCGPAQCQETIRTALAMGADRGIHVEVPPAEAERLGPLQVARVLAKLAEKEKVDLVLLGKQAIDDDCNQTGQMTAGFLDWPQGTFASQVTLEGDKLKVEREIDGGLETLRLKLPAVVTADLRLNEPRYATLPNIMKAKKKKIEVIKPGDLGVDLTSKLSVISVEDPPQRTAGVKVETTEDLVAKLKEIGRI","proteinLength":"Full Length","predictedMolecularWeight":"30 kDa","actualMolecularWeight":null,"aminoAcidEnd":255,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P38117","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETFB also known as electron transfer flavoprotein subunit beta is a protein that plays an important role in transferring electrons in cellular energy metabolism. This protein has a molecular mass of approximately 27 kDa and is mainly expressed in mitochondria-rich organs such as the liver heart and muscle tissues. This protein pairs with the alpha subunit (ETFA) to form the heterodimeric structure of the electron transfer flavoprotein (ETF) essential for its function. ETFB's presence is significant in tissues with high metabolic demand aligning with its involvement in energy production processes.
Biological function summary

The ETFB protein partners with ETFA to form the complete ETF complex which serves as an important electron transporter in the mitochondria. It accepts electrons from various flavoprotein dehydrogenases and transfers them to the mitochondrial respiratory chain. This process is important for beta-oxidation of fatty acids and catabolism of some amino acids. ETFB ensures proper energy production and cellular respiration and its deficiency can lead to severe metabolic disruptions.

Pathways

Several important biological pathways involve ETFB most notably the fatty acid oxidation pathway and the branched-chain amino acid catabolism. It interacts closely with proteins such as the ACADs (acyl-CoA dehydrogenases) which directly send electrons to ETF in the mitochondria. This interaction allows ETFB to maintain efficient energy conversion processes directly linking its function to metabolic activity and energy homeostasis.

ETFB's dysfunction associates with metabolic disorders like glutaric aciduria type II (GA II) and multiple acyl-CoA dehydrogenase deficiency (MADD). These conditions stem from defects in the electron transport function of the ETF complex leading to energy production impairment. Patients with these disorders may have symptoms ranging from mild to severe metabolic crises. ETFB's disturbance in this context often involves ETFA as both proteins form the ETF complex important for normal metabolic function.
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Specifications

Form

Liquid

Additional notes

ab107152 is purified using conventional chromatography techniques.

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General info

Function

Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase (PubMed : 15159392, PubMed : 15975918, PubMed : 25416781). It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (Probable). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism (PubMed : 12815589, PubMed : 7912128). ETFB binds an AMP molecule that probably has a purely structural role (PubMed : 15159392, PubMed : 15975918, PubMed : 8962055).

Sequence similarities

Belongs to the ETF beta-subunit/FixA family.

Post-translational modifications

Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from acyl-CoA dehydrogenases.

Subcellular localisation

Mitochondrion matrix

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase (PubMed : 15159392, PubMed : 15975918, PubMed : 25416781). It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (Probable). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism (PubMed : 12815589, PubMed : 7912128). ETFB binds an AMP molecule that probably has a purely structural role (PubMed : 15159392, PubMed : 15975918, PubMed : 8962055).
See full target information ETFB
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Product promise

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For full details, please see our Terms & Conditions
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