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AB119454

Recombinant Human ETHE1 protein (His tag N-Terminus)

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Recombinant Human ETHE1 protein (His tag N-Terminus) is a Human Full Length protein, in the 13 to 254 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

HSCO, ETHE1, Ethylmalonic encephalopathy protein 1, Hepatoma subtracted clone one protein, Sulfur dioxygenase ETHE1

1 Images
SDS-PAGE - Recombinant Human ETHE1 protein (His tag N-Terminus) (AB119454)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ETHE1 protein (His tag N-Terminus) (AB119454)

3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O95571

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMLSQRGGSGAPILLRQMFEPVSCTFTYLLGDRESREAVLIDPVLETAPRDAQLIKELGLRLLYAVNTHCHADHITGSGLLRSLLPGCQSVISRLSGAQADLHIEDGDSIRFGRFALETRASPGHTPGCVTFVLNDHSMAFTGDALLIRGCGRTDFQQGCAKTLYHSVHEKIFTLPGDCLIYPAHDYHGFTVSTVEEERTLNPRLTLSCEEFVKIMGNLNLPKPQQIDFAVPANMRCGVQTPTA","proteinLength":"Full Length","predictedMolecularWeight":"29.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":254,"aminoAcidStart":13,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O95571","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ETHE1 also known as persulfide dioxygenase plays an important role in the breakdown of sulfur-containing compounds. It catalyzes the conversion of persulfides to sulfite in the mitochondrial matrix. The protein has an approximate mass of 26 kDa and is strongly expressed in tissues with high metabolic activity such as the liver kidney and muscle. It plays an important role in maintaining cellular function and energy balance by efficiently processing by-products of cysteine metabolism.
Biological function summary

ETHE1 assists in detoxifying potentially harmful sulfur compounds. It forms a part of a complex with sulfide:quinone oxidoreductase (SQR) and thiosulfate sulfurtransferase (TST) to facilitate efficient sulfur metabolism. The importance of ETHE1 lies in its ability to prevent the accumulation of toxic sulfide levels which can impair cellular respiration and energy production. The protein's role as a persulfide dioxygenase places it in a unique position as one of the body's defenses against sulfide toxicity.

Pathways

ETHE1 has significant relevance in the sulfide oxidation pathway and cellular respiration processes. Particularly it interacts with SQR in the mitochondrial matrix to convert hydrogen sulfide to thiosulfate and sulfite which then enter the citric acid cycle. This critical pathway is associated with energy production and efficient cellular function. The cooperation between ETHE1 and SQR ensures that toxic sulfide levels do not rise protecting mitochondrial integrity.

Defects in ETHE1 result in ethylmalonic encephalopathy a severe metabolic disorder. The lack of functional ETHE1 leads to the accumulation of toxic levels of hydrogen sulfide which may disrupt mitochondrial function and energy production. This disorder highlights the interconnectedness of ETHE1 with SQR as impairments in this protein complex can exacerbate the pathological condition. Understanding the role of ETHE1 in this disease helps target therapies aimed at reducing sulfide toxicity.
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Specifications

Form

Liquid

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General info

Function

Sulfur dioxygenase that plays an essential role in hydrogen sulfide catabolism in the mitochondrial matrix. Hydrogen sulfide (H(2)S) is first oxidized by SQRDL, giving rise to cysteine persulfide residues. ETHE1 consumes molecular oxygen to catalyze the oxidation of the persulfide, once it has been transferred to a thiophilic acceptor, such as glutathione (R-SSH). Plays an important role in metabolic homeostasis in mitochondria by metabolizing hydrogen sulfide and preventing the accumulation of supraphysiological H(2)S levels that have toxic effects, due to the inhibition of cytochrome c oxidase. First described as a protein that can shuttle between the nucleus and the cytoplasm and suppress p53-induced apoptosis by sequestering the transcription factor RELA/NFKB3 in the cytoplasm and preventing its accumulation in the nucleus (PubMed : 12398897).

Sequence similarities

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Sulfur dioxygenase that plays an essential role in hydrogen sulfide catabolism in the mitochondrial matrix. Hydrogen sulfide (H(2)S) is first oxidized by SQRDL, giving rise to cysteine persulfide residues. ETHE1 consumes molecular oxygen to catalyze the oxidation of the persulfide, once it has been transferred to a thiophilic acceptor, such as glutathione (R-SSH). Plays an important role in metabolic homeostasis in mitochondria by metabolizing hydrogen sulfide and preventing the accumulation of supraphysiological H(2)S levels that have toxic effects, due to the inhibition of cytochrome c oxidase. First described as a protein that can shuttle between the nucleus and the cytoplasm and suppress p53-induced apoptosis by sequestering the transcription factor RELA/NFKB3 in the cytoplasm and preventing its accumulation in the nucleus (PubMed : 12398897).
See full target information ETHE1
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