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AB114696

Recombinant Human EWSR1/EWS protein (GST tag N-Terminus)

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Recombinant Human EWSR1/EWS protein (GST tag N-Terminus) is a Human Fragment protein, in the 358 to 453 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

EWS, EWSR1, RNA-binding protein EWS, EWS oncogene, Ewing sarcoma breakpoint region 1 protein

1 Images
SDS-PAGE - Recombinant Human EWSR1/EWS protein (GST tag N-Terminus) (AB114696)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human EWSR1/EWS protein (GST tag N-Terminus) (AB114696)

12.5% SDS-PAGE showing ab114696 at approximately 36.19 kDa stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, SDS-PAGE, WB

applications

Biologically active

No

Accession

Q01844

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein).</p>" } } }
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Product details

This product was previously labelled as EWSR1.
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Sequence info

[{"sequence":"SDNSAIYVQGLNDSVTLDDLADFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPPTAKAAVEWFDGKDFQGSKLKVSLARKKPPMNS","proteinLength":"Fragment","predictedMolecularWeight":"36.19 kDa","actualMolecularWeight":null,"aminoAcidEnd":453,"aminoAcidStart":358,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q01844","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EWS protein also known as EWSR1 or 2'1b1' comes from the EWSR1 gene. It is a part of the TET family of proteins and has a molecular weight of about 68 kDa. EWS is expressed widely in human tissues with high levels in the brain gonads and skeletal muscle. The protein contains an RNA-binding domain and amino acid repeats which allow it to interact with other proteins and RNA molecules.
Biological function summary

The EWS protein plays roles in various cellular processes such as transcription and RNA splicing. It can participate in forming different protein complexes which influence its function. EWS serves as a cofactor for several transcription factors and it is important in regulating the expression of genes involved in cell cycle progression and differentiation. The protein’s interaction with RNA and other molecules highlights its multifunctional nature within cellular environments.

Pathways

EWS engages in pathways like the regulation of transcription and RNA processing. It closely associates with proteins involved in gene expression surveillance such as the RNA polymerase II which is significant for transcription activities. EWS partners with proteins such as FUS and TAF15 which are essential parts of transcription regulation networks. These interactions place EWS in a central role in cellular regulatory machinery.

EWS is notably linked to Ewing sarcoma and neurodegenerative diseases. In Ewing sarcoma EWS forms fusion proteins like EWS-FLI1 by translocation events which lead to tumor formation. This fusion protein acts abnormally in transcription regulation driving oncogenic pathways. EWS's interaction with proteins like FUS and TAF15 also relate it to certain neurodegenerative conditions indicating its importance in both cancer and neurobiology.
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Specifications

Form

Liquid

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General info

Function

Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3' (PubMed : 21256132). Might normally function as a transcriptional repressor (PubMed : 10767297). EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Sequence similarities

Belongs to the RRM TET family.

Post-translational modifications

Phosphorylated; calmodulin-binding inhibits phosphorylation of Ser-266.. Highly methylated on arginine residues. Methylation is mediated by PRMT1 and, at lower level by PRMT8.

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Product protocols

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Target data

Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3' (PubMed : 21256132). Might normally function as a transcriptional repressor (PubMed : 10767297). EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.
See full target information EWSR1
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Product promise

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