Recombinant Human EXOSC3 protein is a Human Full Length protein, in the 1 to 275 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A E P A S V A A E S L A G S R A R A A R T V L G Q V V L P G E E L L L P E Q E D A E G P G G A V E R P L S L N A R A C S R V R V V C G P G L R R C G D R L L V T K C G R L R H K E P G S G S G G G V Y W V D S Q Q K R Y V P V K G D H V I G I V T A K S G D I F K V D V G G S E P A S L S Y L S F E G A T K R N R P N V Q V G D L I Y G Q F V V A N K D M E P E M V C I D S C G R A N G M G V I G Q D G L L F K V T L G L I R K L L A P D C E I I Q E V G K L Y P L E I V F G M N G R I W V K A K T I Q Q T L I L A N I L E A C E H M T S D Q R K Q I F S R L A E S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC9 and EXOSC5.
Alternative names
RRP40, CGI-102, EXOSC3, Exosome complex component RRP40, Exosome component 3, Ribosomal RNA-processing protein 40, p10
Recommended products
Recombinant Human EXOSC3 protein is a Human Full Length protein, in the 1 to 275 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9NQT5-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A E P A S V A A E S L A G S R A R A A R T V L G Q V V L P G E E L L L P E Q E D A E G P G G A V E R P L S L N A R A C S R V R V V C G P G L R R C G D R L L V T K C G R L R H K E P G S G S G G G V Y W V D S Q Q K R Y V P V K G D H V I G I V T A K S G D I F K V D V G G S E P A S L S Y L S F E G A T K R N R P N V Q V G D L I Y G Q F V V A N K D M E P E M V C I D S C G R A N G M G V I G Q D G L L F K V T L G L I R K L L A P D C E I I Q E V G K L Y P L E I V F G M N G R I W V K A K T I Q Q T L I L A N I L E A C E H M T S D Q R K Q I F S R L A E S
- Accession
- Q9NQT5
- Protein length
- Full Length
- Predicted molecular weight
- 32 kDa
- Amino acids
- 1 to 275
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab181889 was purified using conventional chromatography techniques.
General info
- Function
Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC9 and EXOSC5.
- Sequence similarities
Belongs to the RRP40 family.
- Subcellular localisation
- Nucleus, Nucleolus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EXOSC3 also known as exosome component 3 is a core part of the RNA exosome complex. This protein weighs approximately 33 kDa and is located in both the nucleus and cytoplasm of eukaryotic cells. EXOSC3 along with other exosome components is highly expressed in tissues with rapid cell division and RNA turnover such as the bone marrow and spleen. These expression patterns are consistent with its function in RNA processing and degradation.
- Biological function summary
EXOSC3 plays a critical role in determining RNA stability as part of the exosome complex. This multi-subunit complex degrades various RNA species including mRNA rRNA and snRNA thereby maintaining cellular RNA quality and quantity. EXOSC3 in conjunction with other exosome components executes an important function in RNA surveillance and quality control. Through exosomal complexes EXOSC3 influences many processes that are necessary for cell growth and homeostasis.
- Pathways
The exosome component EXOSC3 shows involvement in the RNA processing pathway and the nuclear mRNA catabolic pathway. This protein collaborates with other exosome components like EXOSC10 to perform precise RNA surveillance. The complex carefully regulates RNA processing events within these pathways to ensure cellular homeostasis and proper gene expression regulation.
- Associated diseases and disorders
Mutations or malfunctions in EXOSC3 link to specific conditions notably pontocerebellar hypoplasia type 1B and spinal muscular atrophy. In particular through its role in RNA metabolism alterations in EXOSC3's function can lead to motor neuron dysfunction. EXOSC3's correlation with EXOSC8 another exosome component can exacerbate symptoms associated with these neural disorders shedding light on the importance of precise RNA processing in neurological health.
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SDS-PAGE - Recombinant Human EXOSC3 protein (ab181889)
15% SDS-PAGE analysis of 3ug ab181889.
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