Recombinant Human EXOSC5/CML28 protein is a Human Full Length protein, in the 1 to 235 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Publications
Filter by
- Cellular physiology and biochemistry : internation 31:659-722013Klotho ameliorates chemically induced endoplasmic reticulum (ER) stress signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSrijita Banerjee et. al.PubMed 23711492
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M M E E E T H T D A K I R A E N G T G S S P R G P G C S L R H F A C E Q N L L S R P D G S A S F L Q G D T S V L A G V Y G P A E V K V S K E I F N K A T L E V I L R P K I G L P G V A E K S R E R L I R N T C E A V V L G T L H P R T S I T V V L Q V V S D A G S L L A C C L N A A C M A L V D A G V P M R A L F C G V A C A L D S D G T L V L D P T S K Q E K E A R A V L T F A L D S V E R K L L M S S T K G L Y S D T E L Q Q C L A A A Q A A S Q H V F R F Y R E S L Q R R Y S K S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes (PubMed:11782436, PubMed:21269460). In vitro, EXOSC5 does not bind or digest single-stranded RNA and binds to double-stranded DNA without detectable DNase activity (PubMed:20660080).
Alternative names
CML28, RRP46, EXOSC5, Exosome complex component RRP46, Chronic myelogenous leukemia tumor antigen 28, Exosome component 5, Ribosomal RNA-processing protein 46, p12B
Recommended products
Recombinant Human EXOSC5/CML28 protein is a Human Full Length protein, in the 1 to 235 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9NQT4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M M E E E T H T D A K I R A E N G T G S S P R G P G C S L R H F A C E Q N L L S R P D G S A S F L Q G D T S V L A G V Y G P A E V K V S K E I F N K A T L E V I L R P K I G L P G V A E K S R E R L I R N T C E A V V L G T L H P R T S I T V V L Q V V S D A G S L L A C C L N A A C M A L V D A G V P M R A L F C G V A C A L D S D G T L V L D P T S K Q E K E A R A V L T F A L D S V E R K L L M S S T K G L Y S D T E L Q Q C L A A A Q A A S Q H V F R F Y R E S L Q R R Y S K S
- Accession
- Q9NQT4
- Protein length
- Full Length
- Predicted molecular weight
- 27.5 kDa
- Amino acids
- 1 to 235
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab124595 is purified using conventional chromatography techniques.
General info
- Function
Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes (PubMed:11782436, PubMed:21269460). In vitro, EXOSC5 does not bind or digest single-stranded RNA and binds to double-stranded DNA without detectable DNase activity (PubMed:20660080).
- Sequence similarities
Belongs to the RNase PH family.
- Subcellular localisation
- Nucleus, Nucleolus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as EXOSC5
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EXOSC5 also known as CML28 is a core component of the exosome complex an important multiprotein machinery involved in RNA processing and degradation. EXOSC5 has a molecular mass of approximately 34 kDa and is expressed in a wide range of tissues. It functions as a scaffold within the exosome complex interacting with other exosome subunits to maintain the stability and structure of the complex.
- Biological function summary
EXOSC5 is important for the normal function of the RNA exosome complex which plays a central role in 3'-to-5' RNA processing and degradation. The exosome complex is essential for removing defective RNA molecules and managing RNA stability. EXOSC5 acts as a bridge between the core exosome and additional cofactors that enhance the exosome's specific functions. This regulation ensures appropriate cellular RNA levels impacting gene expression and integrity.
- Pathways
EXOSC5 is involved in the RNA surveillance pathway which monitors and degrades aberrant RNA transcripts in the cell. The pathway ensures that only properly processed RNAs are translated into proteins. Within this framework EXOSC5 interacts with RRP44 a catalytic component of the exosome bolstering its activity in degrading faulty RNAs. The balance of RNA processing and degradation that EXOSC5 supports is vital in maintaining cellular homeostasis.
- Associated diseases and disorders
Abnormalities in EXOSC5 have been linked to autoimmune diseases and cancers. Autoimmune responses may target EXOSC5 leading to disrupted RNA processing. Moreover alterations in EXOSC5 expression or function can contribute to oncogenesis by impacting the cell cycle and apoptosis. In cancers interactions between EXOSC5 and the protein p53 suggest a connection in cell survival pathways highlighting EXOSC5's potential as a therapeutic target in cancer treatments.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human EXOSC5/CML28 protein (ab124595)
15% SDS-PAGE showing ab124595 (3μg).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com