Recombinant human EZH1 + EED + SUZ12 + RBBP4 + AEBP2 protein (Active) is a Human Full Length protein, in the 2 to 425 aa range, expressed in Baculovirus infected Sf9, with >=79% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >=79% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
A D K E A A F D D A V E E R V I N E E Y K I W K K N T P F L Y D L V M T H A L E W P S L T A Q W L P D V T R P E G K D F S I H R L V L G T H T S D E Q N H L V I A S V Q L P N D D A Q F D A S H Y D S E K G E F G G F G S V S G K I E I E I K I N H E G E V N R A R Y M P Q N P C I I A T K T P S S D V L V F D Y T K H P S K P D P S G E C N P D L R L R G H Q K E G Y G L S W N P N L S G H L L S A S D D H T I C L W D I S A V P K E G K V V D A K T I F T G H T A V V E D V S W H L L H E S L F G S V A D D Q K L M I W D T R S N N T S K P S H S V D A H T A E V N C L S F N P Y S E F I L A T G S A D K T V A L W D L R N L K L K L H S F E S H K D E I F Q V Q W S P H N E T I L A S S G T D R R L N V W D L S K I G E E Q S P E D A E D G P P E L L F I H G G H T A K I S D F S W N P N E P W V I C S V S E D N I M Q V W Q M A E N I Y N D E D P E G S V D P E G Q G S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
Additional Targets
EED, SUZ12, RBBP4, Zinc finger protein AEBP2
Alternative names
RBAP48, RBBP4, Histone-binding protein RBBP4, Chromatin assembly factor 1 subunit C, Chromatin assembly factor I p48 subunit, Nucleosome-remodeling factor subunit RBAP48, Retinoblastoma-binding protein 4, Retinoblastoma-binding protein p48, CAF-1 subunit C, CAF-I 48 kDa subunit, CAF-I p48, RBBP-4
Recommended products
Recombinant human EZH1 + EED + SUZ12 + RBBP4 + AEBP2 protein (Active) is a Human Full Length protein, in the 2 to 425 aa range, expressed in Baculovirus infected Sf9, with >=79% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=79% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- 50 μl reaction mix (20 mM phosphate pH 7.4, 0.05% Tween-20, 40 μM S-adenosylmethionine, and ab196413 were added to the wells coated with the substrate on a Neutravidin white plate. Incubated for 1 hr. Added antibody against methylated K27 residue of histone H3, incubated 1 hr. Then, added secondary HRP-labeled antibody and incubated 30 min. Finally, added HRP chemiluminescent substrates and read luminescence.
- Accession
- Q09028-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Preservative: 1.36% Imidazole
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride
Sequence info
Amino acid sequence 1
- A D K E A A F D D A V E E R V I N E E Y K I W K K N T P F L Y D L V M T H A L E W P S L T A Q W L P D V T R P E G K D F S I H R L V L G T H T S D E Q N H L V I A S V Q L P N D D A Q F D A S H Y D S E K G E F G G F G S V S G K I E I E I K I N H E G E V N R A R Y M P Q N P C I I A T K T P S S D V L V F D Y T K H P S K P D P S G E C N P D L R L R G H Q K E G Y G L S W N P N L S G H L L S A S D D H T I C L W D I S A V P K E G K V V D A K T I F T G H T A V V E D V S W H L L H E S L F G S V A D D Q K L M I W D T R S N N T S K P S H S V D A H T A E V N C L S F N P Y S E F I L A T G S A D K T V A L W D L R N L K L K L H S F E S H K D E I F Q V Q W S P H N E T I L A S S G T D R R L N V W D L S K I G E E Q S P E D A E D G P P E L L F I H G G H T A K I S D F S W N P N E P W V I C S V S E D N I M Q V W Q M A E N I Y N D E D P E G S V D P E G Q G S
- Accession
- Q09028
- Protein length
- Full Length
- Amino acids
- 2 to 425
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Amino acid sequence 2
- S E R E V S T A P A G T D M P A A K K Q K L S S D E N S N P D L S G D E N D D A V S I E S G T N T E R P D T P T N T P N A P G R K S W G K G K W K S K K C K Y S F K C V N S L K E D H N Q P L F G V Q F N W H S K E G D P L V F A T V G S N R V T L Y E C H S Q G E I R L L Q S Y V D A D A D E N F Y T C A W T Y D S N T S H P L L A V A G S R G I I R I I N P I T M Q C I K H Y V G H G N A I N E L K F H P R D P N L L L S V S K D H A L R L W N I Q T D T L V A I F G G V E G H R D E V L S A D Y D L L G E K I M S C G M D H S L K L W R I N S K R M M N A I K E S Y D Y N P N K T N R P F I S Q K I H F P D F S T R D I H R N Y V D C V R W L G D L I L S K S C E N A I V C W K P G K M E D D I D K I K P S E S N V T I L G R F D Y S Q C D I W Y M R F S M D F W Q K M L A L G N Q V G K L Y V W D L E V E D P H K A K C T T L T H H K C G A A I R Q T S F S R D S S I L I A V C D D A S I W R W D R L R
- Accession
- O75530
- Protein length
- Full Length
- Amino acids
- 2 to 441
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Amino acid sequence 3
- A P Q K H G G G G G G G S G P S A G S G G G G F G G S A A V A A A T A S G G K S G G G S C G G G G S Y S A S S S S S A A A A A G A A V L P V K K P K M E H V Q A D H E L F L Q A F E K P T Q I Y R F L R T R N L I A P I F L H R T L T Y M S H R N S R T N I K R K T F K V D D M L S K V E K M K G E Q E S H S L S A H L Q L T F T G F F H K N D K P S P N S E N E Q N S V T L E V L L V K V C H K K R K D V S C P I R Q V P T G K K Q V P L N P D L N Q T K P G N F P S L A V S S N E F E P S N S H M V K S Y S L L F R V T R P G R R E F N G M I N G E T N E N I D V N E E L P A R R K R N R E D G E K T F V A Q M T V F D K N R R L Q L L D G E Y E V A M Q E M E E C P I S K K R A T W E T I L D G K R L P P F E T F S Q G P T L Q F T L R W T G E T N D K S T A P I A K P L A T R N S E S L H Q E N K P G S V K P T Q T I A V K E S L T T D L Q T R K E K D T P N E N R Q K L R I F Y Q F L Y N N N T R Q Q T E A R D D L H C P W C T L N C R K L Y S L L K H L K L C H S R F I F N Y V Y H P K G A R I D V S I N E C Y D G S Y A G N P Q D I H R Q P G F A F S R N G P V K R T P I T H I L V C R P K R T K A S M S E F L E S E D G E V E Q Q R T Y S S G H N R L Y F H S D T C L P L R P Q E M E V D S E D E K D P E W L R E K T I T Q I E E F S D V N E G E K E V M K L W N L H V M K H G F I A D N Q M N H A C M L F V E N Y G Q K I I K K N L C R N F M L H L V S M H D F N L I S I M S I D K A V T K L R E M Q Q K L E K G E S A S P A N E E I T E E Q N G T A N G F S E I N S K E K A L E T D S V S G V S K Q S K K Q K L
- Accession
- Q15022
- Protein length
- Full Length
- Amino acids
- 2 to 739
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Amino acid sequence 4
- A A A I T D M A D L E E L S R L S P L P P G S P G S A A R G R A E P P E E E E E E E E E E E E A E A E A V A A L L L N G G S G G G G G G G G G G V G G G E A E T M S E P S P E S A S Q A G E D E D E E E D D E E E E D E S S S S G G G E E E S S A E S L V G S S G G S S S D E T R S L S P G A A S S S S G D G D G K E G L E E P K G P R G S Q G G G G G G S S S S S V V S S G G D E G Y G T G G G G S S A T S G G R R G S L E M S S D G E P L S R M D S E D S I S S T I M D V D S T I S S G R S T P A M M N G Q G S T T S S S K N I A Y N C C W D Q C Q A C F N S S P D L A D H I R S I H V D G Q R G G V F V C L W K G C K V Y N T P S T S Q S W L Q R H M L T H S G D K P F K C V V G G C N A S F A S Q G G L A R H V P T H F S Q Q N S S K V S S Q P K A K E E S P S K A G M N K R R K L K N K R R R S L P R P H D F F D A Q T L D A I R H R A I C F N L S A H I E S L G K G H S V V F H S T V I A K R K E D S G K I K L L L H W M P E D I L P D V W V N E S E R H Q L K T K V V H L S K L P K D T A L L L D P N I Y R T M P Q K R L K R
- Accession
- Q6ZN18
- Protein length
- Full Length
- Amino acids
- 2 to 503
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Amino acid sequence 5
- E I P N P P T S K C I T Y W K R K V K S E Y M R L R Q L K R L Q A N M G A K A L Y V A N F A K V Q E K T Q I L N E E W K K L R V Q P V Q S M K P V S G H P F L K K C T I E S I F P G F A S Q H M L M R S L N T V A L V P I M Y S W S P L Q Q N F M V E D E T V L C N I P Y M G D E V K E E D E T F I E E L I N N Y D G K V H G E E E M I P G S V L I S D A V F L E L V D A L N Q Y S D E E E E G H N D T S D G K Q D D S K E D L P V T R K R K R H A I E G N K K S S K K Q F P N D M I F S A I A S M F P E N G V P D D M K E R Y R E L T E M S D P N A L P P Q C T P N I D G P N A K S V Q R E Q S L H S F H T L F C R R C F K Y D C F L H P F H A T P N V Y K R K N K E I K I E P E P C G T D C F L L L E G A K E Y A M L H N P R S K C S G R R R R R H H I V S A S C S N A S A S A V A E T K E G D S D R D T G N D W A S S S S E A N S R C Q T P T K Q K A S P A P P Q L C V V E A P S E P V E W T G A E E S L F R V F H G T Y F N N F C S I A R L L G T K T C K Q V F Q F A V K E S L I L K L P T D E L M N P S Q K K K R K H R L W A A H C R K I Q L K K D N S S T Q V Y N Y Q P C D H P D R P C D S T C P C I M T Q N F C E K F C Q C N P D C Q N R F P G C R C K T Q C N T K Q C P C Y L A V R E C D P D L C L T C G A S E H W D C K V V S C K N C S I Q R G L K K H L L L A P S D V A G W G T F I K E S V Q K N E F I S E Y C G E L I S Q D E A D R R G K V Y D K Y M S S F L F N L N N D F V V D A T R K G N K I R F A N H S V N P N C Y A K V V M V N G D H R I G I F A K R A I Q A G E E L F F D Y R Y S Q A D A L K Y V G I E R E T D V L
- Accession
- Q92800
- Protein length
- Full Length
- Amino acids
- 2 to 747
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
- Sequence similarities
Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The EZH1 EED SUZ12 RBBP4 and AEBP2 proteins form the core of Polycomb Repressive Complex 2 (PRC2) which is an essential multi-subunit protein assembly. The main function of PRC2 lies in its ability to methylate histone H3 on lysine 27 (H3K27me3) an important histone modification promoting chromatin compaction and gene silencing. The molecular weight of each subunit varies with EZH1 around 85 kDa. The PRC2 components such as EZH1 mostly express in tissues where epigenetic regulation is required including embryonic stem cells and various somatic tissues.
- Biological function summary
This complex plays a significant role in maintaining transcriptional repression across the genome contributing to cellular identity and stem cell pluripotency. PRC2 including the catalytic subunit EZH1 and its accessory proteins EED SUZ12 RBBP4 and AEBP2 facilitates chromatin remodeling and maintenance of gene silencing by regulating the histone methylation status. This biological mechanism influences differentiation pathways and is important in preserving a balance between cell proliferation and specialization.
- Pathways
EZH1 and its associated complex modulate important developmental and signaling pathways such as the Wnt and Notch pathways. PRC2 works closely with other chromatin modifiers and regulators like HDAC1/2 contributing to the fine-tuning of gene expression during development and cellular differentiation. These pathways are integral to the regulation of cell fate decisions and PRC2's activity ensures proper pathway signaling through chromatin-mediated control.
- Associated diseases and disorders
Abnormalities in EZH1 and associated PRC2 components link to certain cancers especially leukemia and breast cancer. Mutations or altered expression levels of PRC2 subunits influence chromatin structure and gene regulation potentially leading to oncogenesis. These components interact with other proteins such as DNMT3A and TET2 which also play roles in epigenetic modifications and cancer development. Understanding the relationship between PRC2 and disease offers potential therapeutic avenues for targeting these pathways in cancer treatment.
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Terms & Conditions.
2 product images
Functional Studies - Recombinant human EZH1 + EED + SUZ12 + RBBP4 + AEBP2 protein (Active) (ab196413)
Specific activity of ab196413.
SDS-PAGE - Recombinant human EZH1 + EED + SUZ12 + RBBP4 + AEBP2 protein (Active) (ab196413)
SDS-PAGE analysis of ab196413.
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