Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 (mutated A677G) protein is a Human Full Length protein, in the 2 to 517 aa range, expressed in Baculovirus infected Sf9, with >=82% purity and suitable for SDS-PAGE, FuncS.
>=82% SDS-PAGE
Baculovirus infected Sf9 cells
His tag N-Terminus
DDDDK tag N-Terminus
SDS-PAGE, FuncS
Yes
This product is comprised of multiple sequences see
A A A I T D M A D L E E L S R L S P L P P G S P G S A A R G R A E P P E E E E E E E E E E E E A E A E A V A A L L L N G G S G G G G G G G G G G V G G G E A E T M S E P S P E S A S Q A G E D E D E E E D D E E E E D E S S S S G G G E E E S S A E S L V G S S G G S S S D E T R S L S P G A A S S S S G D G D G K E G L E E P K G P R G S Q G G G G G G S S S S S V V S S G G D E G Y G T G G G G S S A T S G G R R G S L E M S S D G E P L S R M D S E D S I S S T I M D V D S T I S S G R S T P A M M N G Q G S T T S S S K N I A Y N C C W D Q C Q A C F N S S P D L A D H I R S I H V D G Q R G G V F V C L W K G C K V Y N T P S T S Q S W L Q R H M L T H S G D K P F K C V V G G C N A S F A S Q G G L A R H V P T H F S Q Q N S S K V S S Q P K A K E E S P S K A G M N K R R K L K N K R R R S L P R P H D F F D A Q T L D A I R H R A I C F N L S A H I E S L G K G H S V V F H S T V I A K R K E D S G K I K L L L H W M P E D I L P D V W V N E S E R H Q L K T K V V H L S K L P K D T A L L L D P N I Y R T M P Q K R L K R T L I R K V F N L Y L S K Q
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
SUZ12, AEBP2, RBBP4, EZH2 mutated A677G
Polycomb protein EED, hEED, Embryonic ectoderm development protein, WD protein associating with integrin cytoplasmic tails 1, WAIT-1, EED
Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 (mutated A677G) protein is a Human Full Length protein, in the 2 to 517 aa range, expressed in Baculovirus infected Sf9, with >=82% purity and suitable for SDS-PAGE, FuncS.
>=82% SDS-PAGE
Baculovirus infected Sf9 cells
His tag N-Terminus
DDDDK tag N-Terminus
SDS-PAGE, FuncS
Yes
ab198114 is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
No
Human
pH: 8
Preservative: 0.14% Imidazole
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride
A A A I T D M A D L E E L S R L S P L P P G S P G S A A R G R A E P P E E E E E E E E E E E E A E A E A V A A L L L N G G S G G G G G G G G G G V G G G E A E T M S E P S P E S A S Q A G E D E D E E E D D E E E E D E S S S S G G G E E E S S A E S L V G S S G G S S S D E T R S L S P G A A S S S S G D G D G K E G L E E P K G P R G S Q G G G G G G S S S S S V V S S G G D E G Y G T G G G G S S A T S G G R R G S L E M S S D G E P L S R M D S E D S I S S T I M D V D S T I S S G R S T P A M M N G Q G S T T S S S K N I A Y N C C W D Q C Q A C F N S S P D L A D H I R S I H V D G Q R G G V F V C L W K G C K V Y N T P S T S Q S W L Q R H M L T H S G D K P F K C V V G G C N A S F A S Q G G L A R H V P T H F S Q Q N S S K V S S Q P K A K E E S P S K A G M N K R R K L K N K R R R S L P R P H D F F D A Q T L D A I R H R A I C F N L S A H I E S L G K G H S V V F H S T V I A K R K E D S G K I K L L L H W M P E D I L P D V W V N E S E R H Q L K T K V V H L S K L P K D T A L L L D P N I Y R T M P Q K R L K R T L I R K V F N L Y L S K Q
Full Length
53 kDa
2 to 517
Recombinant
His tag N-Terminus
A D K E A A F D D A V E E R V I N E E Y K I W K K N T P F L Y D L V M T H A L E W P S L T A Q W L P D V T R P E G K D F S I H R L V L G T H T S D E Q N H L V I A S V Q L P N D D A Q F D A S H Y D S E K G E F G G F G S V S G K I E I E I K I N H E G E V N R A R Y M P Q N P C I I A T K T P S S D V L V F D Y T K H P S K P D P S G E C N P D L R L R G H Q K E G Y G L S W N P N L S G H L L S A S D D H T I C L W D I S A V P K E G K V V D A K T I F T G H T A V V E D V S W H L L H E S L F G S V A D D Q K L M I W D T R S N N T S K P S H S V D A H T A E V N C L S F N P Y S E F I L A T G S A D K T V A L W D L R N L K L K L H S F E S H K D E I F Q V Q W S P H N E T I L A S S G T D R R L N V W D L S K I G E E Q S P E D A E D G P P E L L F I H G G H T A K I S D F S W N P N E P W V I C S V S E D N I M Q V W Q M A E N I Y N D E D P E G S V D P E G Q G S
Full Length
48 kDa
2 to 425
Recombinant
His tag N-Terminus
S E R E V S T A P A G T D M P A A K K Q K L S S D E N S N P D L S G D E N D D A V S I E S G T N T E R P D T P T N T P N A P G R K S W G K G K W K S K K C K Y S F K C V N S L K E D H N Q P L F G V Q F N W H S K E G D P L V F A T V G S N R V T L Y E C H S Q G E I R L L Q S Y V D A D A D E N F Y T C A W T Y D S N T S H P L L A V A G S R G I I R I I N P I T M Q C I K H Y V G H G N A I N E L K F H P R D P N L L L S V S K D H A L R L W N I Q T D T L V A I F G G V E G H R D E V L S A D Y D L L G E K I M S C G M D H S L K L W R I N S K R M M N A I K E S Y D Y N P N K T N R P F I S Q K I H F P D F S T R D I H R N Y V D C V R W L G D L I L S K S C E N A I V C W K P G K M E D D I D K I K P S E S N V T I L G R F D Y S Q C D I W Y M R F S M D F W Q K M L A L G N Q V G K L Y V W D L E V E D P H K A K C T T L T H H K C G A A I R Q T S F S R D S S I L I A V C D D A S I W R W D R L R
Full Length
51 kDa
2 to 441
Recombinant
DDDDK tag N-Terminus
Full Length
86 kDa
2 to 751
Recombinant
His tag N-Terminus
A P Q K H G G G G G G G S G P S A G S G G G G F G G S A A V A A A T A S G G K S G G G S C G G G G S Y S A S S S S S A A A A A G A A V L P V K K P K M E H V Q A D H E L F L Q A F E K P T Q I Y R F L R T R N L I A P I F L H R T L T Y M S H R N S R T N I K R K T F K V D D M L S K V E K M K G E Q E S H S L S A H L Q L T F T G F F H K N D K P S P N S E N E Q N S V T L E V L L V K V C H K K R K D V S C P I R Q V P T G K K Q V P L N P D L N Q T K P G N F P S L A V S S N E F E P S N S H M V K S Y S L L F R V T R P G R R E F N G M I N G E T N E N I D V N E E L P A R R K R N R E D G E K T F V A Q M T V F D K N R R L Q L L D G E Y E V A M Q E M E E C P I S K K R A T W E T I L D G K R L P P F E T F S Q G P T L Q F T L R W T G E T N D K S T A P I A K P L A T R N S E S L H Q E N K P G S V K P T Q T I A V K E S L T T D L Q T R K E K D T P N E N R Q K L R I F Y Q F L Y N N N T R Q Q T E A R D D L H C P W C T L N C R K L Y S L L K H L K L C H S R F I F N Y V Y H P K G A R I D V S I N E C Y D G S Y A G N P Q D I H R Q P G F A F S R N G P V K R T P I T H I L V C R P K R T K A S M S E F L E S E D G E V E Q Q R T Y S S G H N R L Y F H S D T C L P L R P Q E M E V D S E D E K D P E W L R E K T I T Q I E E F S D V N E G E K E V M K L W N L H V M K H G F I A D N Q M N H A C M L F V E N Y G Q K I I K K N L C R N F M L H L V S M H D F N L I S I M S I D K A V T K L R E M Q Q K L E K G E S A S P A N E E I T E E Q N G T A N G F S E I N S K E K A L E T D S V S G V S K Q S K K Q K L
Full Length
87 kDa
2 to 739
Recombinant
His tag N-Terminus
Liquid
Affinity purified.
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Belongs to the WD repeat ESC family.
Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines.
Nucleus
Dry Ice
-80°C
-80°C
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
This supplementary information is collated from multiple sources and compiled automatically.
The protein complex EZH2 + EED + SUZ12 + AEBP2 + RBBP4 often referred to as the Polycomb Repressive Complex 2 (PRC2) performs essential functions in chromatin remodeling. EZH2 the catalytic subunit carries out histone methylation specifically trimethylation of lysine 27 on histone H3 (H3K27me3) which leads to transcriptional repression. The full complex is a significant entity with variable mass between 200-700 kDa due to different interacting partners and post-translational modifications. PRC2's component proteins are widely expressed across different tissues playing integral roles in cellular processes.
The PRC2 complex regulates gene silencing important during development and differentiation. It forms a multiprotein complex where EZH2 works intimately with EED and SUZ12 to mediate its methyltransferase activity while AEBP2 and RBBP4 stabilize the complex and assist in DNA binding. The precise modulation of gene expression through PRC2 ensures correct lineage specification maintaining stem cell pluripotency and directing cellular differentiation.
The Polycomb Repressive Complex 2 influences major signaling networks such as the Wnt signaling and cell cycle regulation pathways. In the Wnt signaling pathway PRC2 interacts with other proteins like β-catenin to control transcription of target genes. Additionally in cell cycle regulation PRC2 modifies chromatin structures to repress genes essential for cell division when necessary. These interactions help maintain homeostasis and physiological balance in the cell.
PRC2 is linked to various cancers and developmental syndromes. Dysregulated EZH2 activity through overexpression or mutation often associates with malignancies like prostate cancer and lymphoma where it aberrantly silences tumor suppressor genes. Additionally PRC2 involvement has been noted in Weaver syndrome a developmental disorder connected with mutations in its components. The misregulation of PRC2 affects other proteins and pathways implicating it in complex disease mechanisms.
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Full details and terms and conditions can be found here:
Terms & Conditions.
4-20% SDS-PAGE analysis of ab198114.
Lane 1: 3.16 μg ab198114 enzyme complex
Lane 2: Protein marker
Stained with Coomassie blue.
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