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Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 (mutated A677G) protein is a Human Full Length protein, in the 2 to 517 aa range, expressed in Baculovirus infected Sf9, with >=82% purity and suitable for SDS-PAGE, FuncS.

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Images

SDS-PAGE - Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 (mutated A677G) protein (AB198114), expandable thumbnail

Key facts

Purity

>=82% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His tag N-Terminus
DDDDK tag N-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Sequence

This product is comprised of multiple sequences see

Amino acid sequence 1

A A A I T D M A D L E E L S R L S P L P P G S P G S A A R G R A E P P E E E E E E E E E E E E A E A E A V A A L L L N G G S G G G G G G G G G G V G G G E A E T M S E P S P E S A S Q A G E D E D E E E D D E E E E D E S S S S G G G E E E S S A E S L V G S S G G S S S D E T R S L S P G A A S S S S G D G D G K E G L E E P K G P R G S Q G G G G G G S S S S S V V S S G G D E G Y G T G G G G S S A T S G G R R G S L E M S S D G E P L S R M D S E D S I S S T I M D V D S T I S S G R S T P A M M N G Q G S T T S S S K N I A Y N C C W D Q C Q A C F N S S P D L A D H I R S I H V D G Q R G G V F V C L W K G C K V Y N T P S T S Q S W L Q R H M L T H S G D K P F K C V V G G C N A S F A S Q G G L A R H V P T H F S Q Q N S S K V S S Q P K A K E E S P S K A G M N K R R K L K N K R R R S L P R P H D F F D A Q T L D A I R H R A I C F N L S A H I E S L G K G H S V V F H S T V I A K R K E D S G K I K L L L H W M P E D I L P D V W V N E S E R H Q L K T K V V H L S K L P K D T A L L L D P N I Y R T M P Q K R L K R T L I R K V F N L Y L S K Q

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.

Targets

SUZ12, AEBP2, RBBP4, EZH2 mutated A677G

Alternative names

Recommended products

Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 (mutated A677G) protein is a Human Full Length protein, in the 2 to 517 aa range, expressed in Baculovirus infected Sf9, with >=82% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>=82% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Applications

SDS-PAGE, FuncS

Biological activity

ab198114 is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Accession
O75530-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Preservative: 0.14% Imidazole
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride

Sequence info

Amino acid sequence 1

A A A I T D M A D L E E L S R L S P L P P G S P G S A A R G R A E P P E E E E E E E E E E E E A E A E A V A A L L L N G G S G G G G G G G G G G V G G G E A E T M S E P S P E S A S Q A G E D E D E E E D D E E E E D E S S S S G G G E E E S S A E S L V G S S G G S S S D E T R S L S P G A A S S S S G D G D G K E G L E E P K G P R G S Q G G G G G G S S S S S V V S S G G D E G Y G T G G G G S S A T S G G R R G S L E M S S D G E P L S R M D S E D S I S S T I M D V D S T I S S G R S T P A M M N G Q G S T T S S S K N I A Y N C C W D Q C Q A C F N S S P D L A D H I R S I H V D G Q R G G V F V C L W K G C K V Y N T P S T S Q S W L Q R H M L T H S G D K P F K C V V G G C N A S F A S Q G G L A R H V P T H F S Q Q N S S K V S S Q P K A K E E S P S K A G M N K R R K L K N K R R R S L P R P H D F F D A Q T L D A I R H R A I C F N L S A H I E S L G K G H S V V F H S T V I A K R K E D S G K I K L L L H W M P E D I L P D V W V N E S E R H Q L K T K V V H L S K L P K D T A L L L D P N I Y R T M P Q K R L K R T L I R K V F N L Y L S K Q

Accession

Q6ZN18

Protein length

Full Length

Predicted molecular weight

53 kDa

Amino acids

2 to 517

Nature

Recombinant

Tags

His tag N-Terminus

Amino acid sequence 2

A D K E A A F D D A V E E R V I N E E Y K I W K K N T P F L Y D L V M T H A L E W P S L T A Q W L P D V T R P E G K D F S I H R L V L G T H T S D E Q N H L V I A S V Q L P N D D A Q F D A S H Y D S E K G E F G G F G S V S G K I E I E I K I N H E G E V N R A R Y M P Q N P C I I A T K T P S S D V L V F D Y T K H P S K P D P S G E C N P D L R L R G H Q K E G Y G L S W N P N L S G H L L S A S D D H T I C L W D I S A V P K E G K V V D A K T I F T G H T A V V E D V S W H L L H E S L F G S V A D D Q K L M I W D T R S N N T S K P S H S V D A H T A E V N C L S F N P Y S E F I L A T G S A D K T V A L W D L R N L K L K L H S F E S H K D E I F Q V Q W S P H N E T I L A S S G T D R R L N V W D L S K I G E E Q S P E D A E D G P P E L L F I H G G H T A K I S D F S W N P N E P W V I C S V S E D N I M Q V W Q M A E N I Y N D E D P E G S V D P E G Q G S

Accession

Q09028

Protein length

Full Length

Predicted molecular weight

48 kDa

Amino acids

2 to 425

Nature

Recombinant

Tags

His tag N-Terminus

Amino acid sequence 3

S E R E V S T A P A G T D M P A A K K Q K L S S D E N S N P D L S G D E N D D A V S I E S G T N T E R P D T P T N T P N A P G R K S W G K G K W K S K K C K Y S F K C V N S L K E D H N Q P L F G V Q F N W H S K E G D P L V F A T V G S N R V T L Y E C H S Q G E I R L L Q S Y V D A D A D E N F Y T C A W T Y D S N T S H P L L A V A G S R G I I R I I N P I T M Q C I K H Y V G H G N A I N E L K F H P R D P N L L L S V S K D H A L R L W N I Q T D T L V A I F G G V E G H R D E V L S A D Y D L L G E K I M S C G M D H S L K L W R I N S K R M M N A I K E S Y D Y N P N K T N R P F I S Q K I H F P D F S T R D I H R N Y V D C V R W L G D L I L S K S C E N A I V C W K P G K M E D D I D K I K P S E S N V T I L G R F D Y S Q C D I W Y M R F S M D F W Q K M L A L G N Q V G K L Y V W D L E V E D P H K A K C T T L T H H K C G A A I R Q T S F S R D S S I L I A V C D D A S I W R W D R L R

Accession

O75530

Protein length

Full Length

Predicted molecular weight

51 kDa

Amino acids

2 to 441

Nature

Recombinant

Tags

DDDDK tag N-Terminus

Amino acid sequence 4

Accession

Q15910

Protein length

Full Length

Predicted molecular weight

86 kDa

Amino acids

2 to 751

Nature

Recombinant

Tags

His tag N-Terminus

Amino acid sequence 5

A P Q K H G G G G G G G S G P S A G S G G G G F G G S A A V A A A T A S G G K S G G G S C G G G G S Y S A S S S S S A A A A A G A A V L P V K K P K M E H V Q A D H E L F L Q A F E K P T Q I Y R F L R T R N L I A P I F L H R T L T Y M S H R N S R T N I K R K T F K V D D M L S K V E K M K G E Q E S H S L S A H L Q L T F T G F F H K N D K P S P N S E N E Q N S V T L E V L L V K V C H K K R K D V S C P I R Q V P T G K K Q V P L N P D L N Q T K P G N F P S L A V S S N E F E P S N S H M V K S Y S L L F R V T R P G R R E F N G M I N G E T N E N I D V N E E L P A R R K R N R E D G E K T F V A Q M T V F D K N R R L Q L L D G E Y E V A M Q E M E E C P I S K K R A T W E T I L D G K R L P P F E T F S Q G P T L Q F T L R W T G E T N D K S T A P I A K P L A T R N S E S L H Q E N K P G S V K P T Q T I A V K E S L T T D L Q T R K E K D T P N E N R Q K L R I F Y Q F L Y N N N T R Q Q T E A R D D L H C P W C T L N C R K L Y S L L K H L K L C H S R F I F N Y V Y H P K G A R I D V S I N E C Y D G S Y A G N P Q D I H R Q P G F A F S R N G P V K R T P I T H I L V C R P K R T K A S M S E F L E S E D G E V E Q Q R T Y S S G H N R L Y F H S D T C L P L R P Q E M E V D S E D E K D P E W L R E K T I T Q I E E F S D V N E G E K E V M K L W N L H V M K H G F I A D N Q M N H A C M L F V E N Y G Q K I I K K N L C R N F M L H L V S M H D F N L I S I M S I D K A V T K L R E M Q Q K L E K G E S A S P A N E E I T E E Q N G T A N G F S E I N S K E K A L E T D S V S G V S K Q S K K Q K L

Accession

Q15022

Protein length

Full Length

Predicted molecular weight

87 kDa

Amino acids

2 to 739

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

Additional notes

Affinity purified.

General info

Function

Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.

Sequence similarities

Belongs to the WD repeat ESC family.

Post-translational modifications

Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein complex EZH2 + EED + SUZ12 + AEBP2 + RBBP4 often referred to as the Polycomb Repressive Complex 2 (PRC2) performs essential functions in chromatin remodeling. EZH2 the catalytic subunit carries out histone methylation specifically trimethylation of lysine 27 on histone H3 (H3K27me3) which leads to transcriptional repression. The full complex is a significant entity with variable mass between 200-700 kDa due to different interacting partners and post-translational modifications. PRC2's component proteins are widely expressed across different tissues playing integral roles in cellular processes.

Biological function summary

The PRC2 complex regulates gene silencing important during development and differentiation. It forms a multiprotein complex where EZH2 works intimately with EED and SUZ12 to mediate its methyltransferase activity while AEBP2 and RBBP4 stabilize the complex and assist in DNA binding. The precise modulation of gene expression through PRC2 ensures correct lineage specification maintaining stem cell pluripotency and directing cellular differentiation.

Pathways

The Polycomb Repressive Complex 2 influences major signaling networks such as the Wnt signaling and cell cycle regulation pathways. In the Wnt signaling pathway PRC2 interacts with other proteins like β-catenin to control transcription of target genes. Additionally in cell cycle regulation PRC2 modifies chromatin structures to repress genes essential for cell division when necessary. These interactions help maintain homeostasis and physiological balance in the cell.

Associated diseases and disorders

PRC2 is linked to various cancers and developmental syndromes. Dysregulated EZH2 activity through overexpression or mutation often associates with malignancies like prostate cancer and lymphoma where it aberrantly silences tumor suppressor genes. Additionally PRC2 involvement has been noted in Weaver syndrome a developmental disorder connected with mutations in its components. The misregulation of PRC2 affects other proteins and pathways implicating it in complex disease mechanisms.

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1 product image

  • SDS-PAGE - Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 (mutated A677G) protein (ab198114), expandable thumbnail

    SDS-PAGE - Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 (mutated A677G) protein (ab198114)

    4-20% SDS-PAGE analysis of ab198114.

    Lane 1: 3.16 μg ab198114 enzyme complex
    Lane 2: Protein marker

    Stained with Coomassie blue.

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