Recombinant Human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 (mutated Y646F) protein is a Human Full Length protein, in the 2 to 503 aa range, expressed in Baculovirus infected Sf9, with >=90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus DDDDK tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
A A A I T D M A D L E E L S R L S P L P P G S P G S A A R G R A E P P E E E E E E E E E E E E A E A E A V A A L L L N G G S G G G G G G G G G G V G G G E A E T M S E P S P E S A S Q A G E D E D E E E D D E E E E D E S S S S G G G E E E S S A E S L V G S S G G S S S D E T R S L S P G A A S S S S G D G D G K E G L E E P K G P R G S Q G G G G G G S S S S S V V S S G G D E G Y G T G G G G S S A T S G G R R G S L E M S S D G E P L S R M D S E D S I S S T I M D V D S T I S S G R S T P A M M N G Q G S T T S S S K N I A Y N C C W D Q C Q A C F N S S P D L A D H I R S I H V D G Q R G G V F V C L W K G C K V Y N T P S T S Q S W L Q R H M L T H S G D K P F K C V V G G C N A S F A S Q G G L A R H V P T H F S Q Q N S S K V S S Q P K A K E E S P S K A G M N K R R K L K N K R R R S L P R P H D F F D A Q T L D A I R H R A I C F N L S A H I E S L G K G H S V V F H S T V I A K R K E D S G K I K L L L H W M P E D I L P D V W V N E S E R H Q L K T K V V H L S K L P K D T A L L L D P N I Y R T M P Q K R L K R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Additional Targets
SUZ12, AEBP2, RBBP4, EZH2 mutated Y646F
Alternative names
Polycomb protein EED, hEED, Embryonic ectoderm development protein, WD protein associating with integrin cytoplasmic tails 1, WAIT-1, EED
Recommended products
Recombinant Human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 (mutated Y646F) protein is a Human Full Length protein, in the 2 to 503 aa range, expressed in Baculovirus infected Sf9, with >=90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus DDDDK tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O75530-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.8% Sodium chloride, 0.4% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated
Sequence info
Amino acid sequence 1
- A A A I T D M A D L E E L S R L S P L P P G S P G S A A R G R A E P P E E E E E E E E E E E E A E A E A V A A L L L N G G S G G G G G G G G G G V G G G E A E T M S E P S P E S A S Q A G E D E D E E E D D E E E E D E S S S S G G G E E E S S A E S L V G S S G G S S S D E T R S L S P G A A S S S S G D G D G K E G L E E P K G P R G S Q G G G G G G S S S S S V V S S G G D E G Y G T G G G G S S A T S G G R R G S L E M S S D G E P L S R M D S E D S I S S T I M D V D S T I S S G R S T P A M M N G Q G S T T S S S K N I A Y N C C W D Q C Q A C F N S S P D L A D H I R S I H V D G Q R G G V F V C L W K G C K V Y N T P S T S Q S W L Q R H M L T H S G D K P F K C V V G G C N A S F A S Q G G L A R H V P T H F S Q Q N S S K V S S Q P K A K E E S P S K A G M N K R R K L K N K R R R S L P R P H D F F D A Q T L D A I R H R A I C F N L S A H I E S L G K G H S V V F H S T V I A K R K E D S G K I K L L L H W M P E D I L P D V W V N E S E R H Q L K T K V V H L S K L P K D T A L L L D P N I Y R T M P Q K R L K R
- Accession
- Q6ZN18
- Protein length
- Full Length
- Predicted molecular weight
- 53 kDa
- Amino acids
- 2 to 503
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Amino acid sequence 2
- G Q T G K K S E K G P V C W R K R V K S E Y M R L R Q L K R F R R A D E V K S M F S S N R Q K I L E R T E I L N Q E W K Q R R I Q P V H I L T S V S S L R G T R E C S V T S D L D F P T Q V I P L K T L N A V A S V P I M Y S W S P L Q Q N F M V E D E T V L H N I P Y M G D E V L D Q D G T F I E E L I K N Y D G K V H G D R E C G F I N D E I F V E L V N A L G Q Y N D D D D D D D G D D P E E R E E K Q K D L E D H R D D K E S R P P R K F P S D K I F E A I S S M F P D K G T A E E L K E K Y K E L T E Q Q L P G A L P P E C T P N I D G P N A K S V Q R E Q S L H S F H T L F C R R C F K Y D C F L H R K C N Y S F H A T P N T Y K R K N T E T A L D N K P C G P Q C Y Q H L E G A K E F A A A L T A E R I K T P P K R P G G R R R G R L P N N S S R P S T P T I N V L E S K D T D S D R E A G T E T G G E N N D K E E E E K K D E T S S S S E A N S R C Q T P I K M K P N I E P P E N V E W S G A E A S M F R V L I G T Y Y D N F C A I A R L I G T K T C R Q V Y E F R V K E S S I I A P A P A E D V D T P P R K K K R K H R L W A A H C R K I Q L K K D G S S N H V Y N Y Q P C D H P R Q P C D S S C P C V I A Q N F C E K F C Q C S S E C Q N R F P G C R C K A Q C N T K Q C P C Y L A V R E C D P D L C L T C G A A D H W D S K N V S C K N C S I Q R G S K K H L L L A P S D V A G W G I F I K D P V Q K N E F I S E F C G E I I S Q D E A D R R G K V Y D K Y M C S F L F N L N N D F V V D A T R K G N K I R F A N H S V N P N C Y A K V M M V N G D H R I G I F A K R A I Q T G E E L F F D Y R Y S Q A D A L K Y V G I E R E M E I P
- Accession
- Q15910
- Protein length
- Full Length
- Predicted molecular weight
- 86 kDa
- Amino acids
- 2 to 751
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Amino acid sequence 3
- A D K E A A F D D A V E E R V I N E E Y K I W K K N T P F L Y D L V M T H A L E W P S L T A Q W L P D V T R P E G K D F S I H R L V L G T H T S D E Q N H L V I A S V Q L P N D D A Q F D A S H Y D S E K G E F G G F G S V S G K I E I E I K I N H E G E V N R A R Y M P Q N P C I I A T K T P S S D V L V F D Y T K H P S K P D P S G E C N P D L R L R G H Q K E G Y G L S W N P N L S G H L L S A S D D H T I C L W D I S A V P K E G K V V D A K T I F T G H T A V V E D V S W H L L H E S L F G S V A D D Q K L M I W D T R S N N T S K P S H S V D A H T A E V N C L S F N P Y S E F I L A T G S A D K T V A L W D L R N L K L K L H S F E S H K D E I F Q V Q W S P H N E T I L A S S G T D R R L N V W D L S K I G E E Q S P E D A E D G P P E L L F I H G G H T A K I S D F S W N P N E P W V I C S V S E D N I M Q V W Q M A E N I Y N D E D P E G S V D P E G Q G S
- Accession
- Q09028
- Protein length
- Full Length
- Predicted molecular weight
- 48 kDa
- Amino acids
- 2 to 425
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Amino acid sequence 4
- S E R E V S T A P A G T D M P A A K K Q K L S S D E N S N P D L S G D E N D D A V S I E S G T N T E R P D T P T N T P N A P G R K S W G K G K W K S K K C K Y S F K C V N S L K E D H N Q P L F G V Q F N W H S K E G D P L V F A T V G S N R V T L Y E C H S Q G E I R L L Q S Y V D A D A D E N F Y T C A W T Y D S N T S H P L L A V A G S R G I I R I I N P I T M Q C I K H Y V G H G N A I N E L K F H P R D P N L L L S V S K D H A L R L W N I Q T D T L V A I F G G V E G H R D E V L S A D Y D L L G E K I M S C G M D H S L K L W R I N S K R M M N A I K E S Y D Y N P N K T N R P F I S Q K I H F P D F S T R D I H R N Y V D C V R W L G D L I L S K S C E N A I V C W K P G K M E D D I D K I K P S E S N V T I L G R F D Y S Q C D I W Y M R F S M D F W Q K M L A L G N Q V G K L Y V W D L E V E D P H K A K C T T L T H H K C G A A I R Q T S F S R D S S I L I A V C D D A S I W R W D R L R
- Accession
- O75530
- Protein length
- Full Length
- Predicted molecular weight
- 51 kDa
- Amino acids
- 2 to 441
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Amino acid sequence 5
- A P Q K H G G G G G G G S G P S A G S G G G G F G G S A A V A A A T A S G G K S G G G S C G G G G S Y S A S S S S S A A A A A G A A V L P V K K P K M E H V Q A D H E L F L Q A F E K P T Q I Y R F L R T R N L I A P I F L H R T L T Y M S H R N S R T N I K R K T F K V D D M L S K V E K M K G E Q E S H S L S A H L Q L T F T G F F H K N D K P S P N S E N E Q N S V T L E V L L V K V C H K K R K D V S C P I R Q V P T G K K Q V P L N P D L N Q T K P G N F P S L A V S S N E F E P S N S H M V K S Y S L L F R V T R P G R R E F N G M I N G E T N E N I D V N E E L P A R R K R N R E D G E K T F V A Q M T V F D K N R R L Q L L D G E Y E V A M Q E M E E C P I S K K R A T W E T I L D G K R L P P F E T F S Q G P T L Q F T L R W T G E T N D K S T A P I A K P L A T R N S E S L H Q E N K P G S V K P T Q T I A V K E S L T T D L Q T R K E K D T P N E N R Q K L R I F Y Q F L Y N N N T R Q Q T E A R D D L H C P W C T L N C R K L Y S L L K H L K L C H S R F I F N Y V Y H P K G A R I D V S I N E C Y D G S Y A G N P Q D I H R Q P G F A F S R N G P V K R T P I T H I L V C R P K R T K A S M S E F L E S E D G E V E Q Q R T Y S S G H N R L Y F H S D T C L P L R P Q E M E V D S E D E K D P E W L R E K T I T Q I E E F S D V N E G E K E V M K L W N L H V M K H G F I A D N Q M N H A C M L F V E N Y G Q K I I K K N L C R N F M L H L V S M H D F N L I S I M S I D K A V T K L R E M Q Q K L E K G E S A S P A N E E I T E E Q N G T A N G F S E I N S K E K A L E T D S V S G V S K Q S K K Q K L
- Accession
- Q15022
- Protein length
- Full Length
- Predicted molecular weight
- 87 kDa
- Amino acids
- 2 to 739
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
- Sequence similarities
Belongs to the WD repeat ESC family.
- Post-translational modifications
Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Mutant version of EZH2 5-member complex but with a Tyr-to-Phe mutation on aa646 of the EZH2 protein. Complex of Human EZH2 (NM_004456), aa2-end, Y646F*, with N terminal His tag, MW= 86 kDa, Human EED (NM_003797), aa2-end with N terminal DDDDK tag, MW= 51 kDa, Human SUZ12 (NM_015355), aa2-end with N terminal His tag, MW = 87 kDa, Human AEBP2 (NM_153207), aa2- end with N terminal His tag, MW = 53 kDa, and Human RBBP4 (NM_005610), aa2-end with N terminal His tag, MW = 48 kDa, co-expressed in a baculovirus expression system.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein complex EZH2 + EED + SUZ12 + AEBP2 + RBBP4 often referred to as the Polycomb Repressive Complex 2 (PRC2) performs essential functions in chromatin remodeling. EZH2 the catalytic subunit carries out histone methylation specifically trimethylation of lysine 27 on histone H3 (H3K27me3) which leads to transcriptional repression. The full complex is a significant entity with variable mass between 200-700 kDa due to different interacting partners and post-translational modifications. PRC2's component proteins are widely expressed across different tissues playing integral roles in cellular processes.
- Biological function summary
The PRC2 complex regulates gene silencing important during development and differentiation. It forms a multiprotein complex where EZH2 works intimately with EED and SUZ12 to mediate its methyltransferase activity while AEBP2 and RBBP4 stabilize the complex and assist in DNA binding. The precise modulation of gene expression through PRC2 ensures correct lineage specification maintaining stem cell pluripotency and directing cellular differentiation.
- Pathways
The Polycomb Repressive Complex 2 influences major signaling networks such as the Wnt signaling and cell cycle regulation pathways. In the Wnt signaling pathway PRC2 interacts with other proteins like β-catenin to control transcription of target genes. Additionally in cell cycle regulation PRC2 modifies chromatin structures to repress genes essential for cell division when necessary. These interactions help maintain homeostasis and physiological balance in the cell.
- Associated diseases and disorders
PRC2 is linked to various cancers and developmental syndromes. Dysregulated EZH2 activity through overexpression or mutation often associates with malignancies like prostate cancer and lymphoma where it aberrantly silences tumor suppressor genes. Additionally PRC2 involvement has been noted in Weaver syndrome a developmental disorder connected with mutations in its components. The misregulation of PRC2 affects other proteins and pathways implicating it in complex disease mechanisms.
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1 product image
SDS-PAGE - Recombinant Human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 (mutated Y646F) protein (ab198129)
10% gradient SDS-PAGE analysis of ab198129 (4μg complex).
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