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AB198146

Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 protein (6x His tag N-Term, DDDDK tag N-Term)

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Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 protein (6x His tag N-Term, DDDDK tag N-Term) is a Human Full Length protein, in the 2 to 425 aa range, expressed in Baculovirus infected insect cells, with >96%, suitable for SDS-PAGE, FuncS.

View Alternative Names

RBAP48, RBBP4, Histone-binding protein RBBP4, Chromatin assembly factor 1 subunit C, Chromatin assembly factor I p48 subunit, Nucleosome-remodeling factor subunit RBAP48, Retinoblastoma-binding protein 4, Retinoblastoma-binding protein p48, CAF-1 subunit C, CAF-I 48 kDa subunit, CAF-I p48, RBBP-4, KMT6, EZH2, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, CHET9, JJAZ1, KIAA0160, SUZ12, Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein, Suppressor of zeste 12 protein homolog, ChET 9 protein, Polycomb protein EED, hEED, Embryonic ectoderm development protein, WD protein associating with integrin cytoplasmic tails 1, WAIT-1, EED, Zinc finger protein AEBP2, Adipocyte enhancer-binding protein 2, AE-binding protein 2, AEBP2

2 Images
Functional Studies - Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 protein (6x His tag N-Term, DDDDK tag N-Term) (AB198146)
  • FuncS

Supplier Data

Functional Studies - Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 protein (6x His tag N-Term, DDDDK tag N-Term) (AB198146)

Specific activity of ab198146.

Assay conditions : 50 μL reaction mix (20 mM phosphate buffer pH 7.4, 0.05% Tween-20, 20 μM S-adenosylmethionine, and 50-200 ng enzyme complex) add to the wells coated with the substrate. Incubate for 1 hour. Add antibody against methylated K27 residue of histone H3, incubate 1 hour. Then, add secondary HRP-labeled antibody and incubate 30 minutes. Finally, add HRP chemiluminescent substrates and read luminesence.

SDS-PAGE - Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 protein (6x His tag N-Term, DDDDK tag N-Term) (AB198146)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 protein (6x His tag N-Term, DDDDK tag N-Term) (AB198146)

4-20% gradient SDS-PAGE Coomassie staining.

Lane 1 : 6.2 μg ab198146 enzyme complex

Lane 2 : Protein Marker

Key facts

Purity

>96% SDS-PAGE

Expression system

Baculovirus infected insect cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

0.04 pmol/min/μg

Accession

Q09028

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Preservative: 1.36% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride

storage-buffer

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Complementary Products

Proteins & Peptides

AB132934

Recombinant Human KMT6 / EZH2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human KMT6 / EZH2 protein (GST tag N-Terminus) (AB132934)

  • 0
  • 0
Primary Antibodies

AB240650

Anti-EED antibody [EPR23043-5] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated|Trial Size

Immunoprecipitation - Anti-EED antibody [EPR23043-5] - ChIP Grade (AB240650)

  • 4
  • 5
Proteins & Peptides

AB198157

Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein (His tag N-Terminus, DDDDK tag N-Terminus)

Functional Studies - Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein (His tag N-Terminus, DDDDK tag N-Terminus) (AB198157)

  • 0
  • 0
Primary Antibodies

AB191080

Anti-KMT6 / EZH2 antibody [EPR9307(2)] - N-terminal

RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Anti-KMT6 / EZH2 antibody [EPR9307(2)] - N-terminal (AB191080)

  • 2
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"48 kDa","actualMolecularWeight":null,"aminoAcidEnd":425,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q09028","tags":[{"tag":"6x His","terminus":"N-Terminus"}]},{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"51 kDa","actualMolecularWeight":null,"aminoAcidEnd":441,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O75530","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]},{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"53 kDa","actualMolecularWeight":null,"aminoAcidEnd":503,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q6ZN18","tags":[{"tag":"6x His","terminus":"N-Terminus"}]},{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"86 kDa","actualMolecularWeight":null,"aminoAcidEnd":746,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q15910","tags":[{"tag":"6x His","terminus":"N-Terminus"}]},{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"87 kDa","actualMolecularWeight":null,"aminoAcidEnd":739,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q15022","tags":[{"tag":"6x His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein complex EZH2 + EED + SUZ12 + AEBP2 + RBBP4 often referred to as the Polycomb Repressive Complex 2 (PRC2) performs essential functions in chromatin remodeling. EZH2 the catalytic subunit carries out histone methylation specifically trimethylation of lysine 27 on histone H3 (H3K27me3) which leads to transcriptional repression. The full complex is a significant entity with variable mass between 200-700 kDa due to different interacting partners and post-translational modifications. PRC2's component proteins are widely expressed across different tissues playing integral roles in cellular processes.
Biological function summary

The PRC2 complex regulates gene silencing important during development and differentiation. It forms a multiprotein complex where EZH2 works intimately with EED and SUZ12 to mediate its methyltransferase activity while AEBP2 and RBBP4 stabilize the complex and assist in DNA binding. The precise modulation of gene expression through PRC2 ensures correct lineage specification maintaining stem cell pluripotency and directing cellular differentiation.

Pathways

The Polycomb Repressive Complex 2 influences major signaling networks such as the Wnt signaling and cell cycle regulation pathways. In the Wnt signaling pathway PRC2 interacts with other proteins like β-catenin to control transcription of target genes. Additionally in cell cycle regulation PRC2 modifies chromatin structures to repress genes essential for cell division when necessary. These interactions help maintain homeostasis and physiological balance in the cell.

PRC2 is linked to various cancers and developmental syndromes. Dysregulated EZH2 activity through overexpression or mutation often associates with malignancies like prostate cancer and lymphoma where it aberrantly silences tumor suppressor genes. Additionally PRC2 involvement has been noted in Weaver syndrome a developmental disorder connected with mutations in its components. The misregulation of PRC2 affects other proteins and pathways implicating it in complex disease mechanisms.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA (PubMed : 10866654). Component of the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair (PubMed : 8858152). Component of the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression (PubMed : 9150135). Component of the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling (PubMed : 16428440, PubMed : 28977666, PubMed : 39460621). Component of the PRC2 complex, which promotes repression of homeotic genes during development (PubMed : 29499137, PubMed : 31959557). Component of the NURF (nucleosome remodeling factor) complex (PubMed : 14609955, PubMed : 15310751).

Sequence similarities

Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.

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Product protocols

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Target data

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA (PubMed : 10866654). Component of the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair (PubMed : 8858152). Component of the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression (PubMed : 9150135). Component of the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling (PubMed : 16428440, PubMed : 28977666, PubMed : 39460621). Component of the PRC2 complex, which promotes repression of homeotic genes during development (PubMed : 29499137, PubMed : 31959557). Component of the NURF (nucleosome remodeling factor) complex (PubMed : 14609955, PubMed : 15310751).
See full target information RBBP4

Additional targets

EED,SUZ12,AEBP2,EZH2
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