JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB196409

Recombinant human EZH2 + EED + SUZ12 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human EZH2 + EED + SUZ12 protein is a Human Full Length protein, in the 2 to 739 aa range, expressed in Baculovirus infected Sf9 cells, with >82%, suitable for SDS-PAGE, FuncS.

View Alternative Names

CHET9, JJAZ1, KIAA0160, SUZ12, Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein, Suppressor of zeste 12 protein homolog, ChET 9 protein

2 Images
Functional Studies - Recombinant human EZH2 + EED + SUZ12 protein (AB196409)
  • FuncS

Supplier Data

Functional Studies - Recombinant human EZH2 + EED + SUZ12 protein (AB196409)

Activity assay of ab196409.

SDS-PAGE - Recombinant human EZH2 + EED + SUZ12 protein (AB196409)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human EZH2 + EED + SUZ12 protein (AB196409)

4-20% SDS-PAGE analysis of 3.9 μg ab196409 with Coomassie staining.

Key facts

Purity

>82% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: 0.08 pmole/min/μg.

Assay conditions; 50 μl reaction mix (20 mM phosphate pH 7.4, 0.05% Tween-20, 40 μM S-adenosylmethionine, and 250-2000 ng ab196409 is added to the wells coated with histone H3 substrate on a Neutravidin black plate (ThermoFisher). Incubate for 1 hr. Add antibody against methylated K27 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Add HRP chemiluminescent substrates and read luminescence.

Accession

Q15022

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Complex of Human EZH2 (NM_004456), aa2-end with N-terminal His tag, MW= 86 kDa, Human EED (NM_003797), aa2-end with N-terminal DDDDK tag, MW= 51 kDa, and Human SUZ12 (NM_015355), aa2-end with N-terminal His tag, MW = 87 kDa, co-expressed in a baculovirus expression system.
style
left-column

Sequence info

[{"sequence":"APQKHGGGGGGGSGPSAGSGGGGFGGSAAVAAATASGGKSGGGSCGGGGSYSASSSSSAAAAAGAAVLPVKKPKMEHVQADHELFLQAFEKPTQIYRFLRTRNLIAPIFLHRTLTYMSHRNSRTNIKRKTFKVDDMLSKVEKMKGEQESHSLSAHLQLTFTGFFHKNDKPSPNSENEQNSVTLEVLLVKVCHKKRKDVSCPIRQVPTGKKQVPLNPDLNQTKPGNFPSLAVSSNEFEPSNSHMVKSYSLLFRVTRPGRREFNGMINGETNENIDVNEELPARRKRNREDGEKTFVAQMTVFDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFETFSQGPTLQFTLRWTGETNDKSTAPIAKPLATRNSESLHQENKPGSVKPTQTIAVKESLTTDLQTRKEKDTPNENRQKLRIFYQFLYNNNTRQQTEARDDLHCPWCTLNCRKLYSLLKHLKLCHSRFIFNYVYHPKGARIDVSINECYDGSYAGNPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEVEQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIEEFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKNLCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEITEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL","proteinLength":"Full Length","predictedMolecularWeight":"87 kDa","actualMolecularWeight":null,"aminoAcidEnd":739,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q15022","tags":[{"tag":"His","terminus":"N-Terminus"}]},{"sequence":"SEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR","proteinLength":"Full Length","predictedMolecularWeight":"51 kDa","actualMolecularWeight":null,"aminoAcidEnd":441,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O75530","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]},{"sequence":"GQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHRKCNYSFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVLESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIP","proteinLength":"Full Length","predictedMolecularWeight":"86 kDa","actualMolecularWeight":null,"aminoAcidEnd":751,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q15910","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EZH2 + EED + SUZ12 complex also called the Polycomb Repressive Complex 2 (PRC2) plays a significant role in gene silencing through methylation of histone H3 at lysine 27 (H3K27me3). EZH2 acts as the catalytic subunit while EED and SUZ12 stabilize and enhance its catalytic activity. The mass of EZH2 is approximately 83 kDa. This complex is usually expressed in the nucleus where it performs its function in epigenetic regulation.
Biological function summary

PRC2 is essential for maintaining the silenced state of target genes. It acts as a part of the Polycomb-group (PcG) proteins which are important for transcriptional repression. Through their activity these complexes preserve the repression of developmental genes and prevent abnormal activation during cell differentiation and proliferation. PRC2 often teams up with other chromatin modifiers and DNA-binding proteins to accurately target specific genomic loci.

Pathways

EZH2 + EED + SUZ12 functions in the epigenetic regulatory pathway influencing major signaling pathways such as the Wnt and Notch pathways. Through its role in these pathways PRC2 regulates gene expression and is directly tied to critical cellular processes like cell cycle control and differentiation. Other proteins such as members of the SWI/SNF complex often counteract the function of PRC2 to balance gene expression patterns.

The abnormal regulation of EZH2 + EED + SUZ12 links to cancer and developmental disorders. Overexpression or mutation of EZH2 contributes to oncogenic processes in several types of cancer including prostate cancer. In the context of these diseases PRC2 interacts with additional oncogenic factors such as MYC promoting tumor progression. Furthermore disruptions in PRC2 function have connections to developmental disorders like Weaver syndrome through improper regulation of growth-regulating genes.
style
left-column

Specifications

Form

Liquid

Additional notes

Affinity purified.

style
left-column

General info

Function

Polycomb group (PcG) protein. Component of the PRC2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene (PubMed : 15225548, PubMed : 15231737, PubMed : 15385962, PubMed : 16618801, PubMed : 17344414, PubMed : 18285464, PubMed : 28229514, PubMed : 29499137, PubMed : 31959557). The PRC2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (PubMed : 12351676, PubMed : 12435631, PubMed : 15099518, PubMed : 15225548, PubMed : 15385962, PubMed : 15684044, PubMed : 16431907, PubMed : 18086877, PubMed : 18285464). Genes repressed by the PRC2 complex include HOXC8, HOXA9, MYT1 and CDKN2A (PubMed : 15231737, PubMed : 16618801, PubMed : 17200670, PubMed : 31959557).

Sequence similarities

Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.

Post-translational modifications

Sumoylated, probably by PIAS2.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Polycomb group (PcG) protein. Component of the PRC2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene (PubMed : 15225548, PubMed : 15231737, PubMed : 15385962, PubMed : 16618801, PubMed : 17344414, PubMed : 18285464, PubMed : 28229514, PubMed : 29499137, PubMed : 31959557). The PRC2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (PubMed : 12351676, PubMed : 12435631, PubMed : 15099518, PubMed : 15225548, PubMed : 15385962, PubMed : 15684044, PubMed : 16431907, PubMed : 18086877, PubMed : 18285464). Genes repressed by the PRC2 complex include HOXC8, HOXA9, MYT1 and CDKN2A (PubMed : 15231737, PubMed : 16618801, PubMed : 17200670, PubMed : 31959557).
See full target information SUZ12

Additional targets

EZH2,SUZ12,SUZ12
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB132934

Recombinant Human KMT6 / EZH2 protein

SDS-PAGE - Recombinant Human KMT6 / EZH2 protein (AB132934)

  • 0
  • 0
Primary Antibodies

AB240650

Anti-EED antibody [EPR23043-5] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated|Trial Size

ChIP-sequencing - Anti-EED antibody [EPR23043-5] - ChIP Grade (AB240650)

  • 4
  • 5
Proteins & Peptides

AB198157

Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein

Functional Studies - Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein (AB198157)

  • 0
  • 0
Primary Antibodies

AB191080

Anti-KMT6 / EZH2 antibody [EPR9307(2)] - N-terminal

RabMAb|Recombinant|KO Validated|Trial Size

Western blot - Anti-KMT6 / EZH2 antibody [EPR9307(2)] - N-terminal (AB191080)

  • 2
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com