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AB198127

Recombinant Human EZH2 + SUZ12 + RbAp48 protein (His tag N-Term, His tag N-Term + GST tag N-Term)

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Recombinant Human EZH2 + SUZ12 + RbAp48 protein (His tag N-Term, His tag N-Term + GST tag N-Term) is a Human Full Length protein, in the 2 to 425 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

CHET9, JJAZ1, KIAA0160, SUZ12, Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein, Suppressor of zeste 12 protein homolog, ChET 9 protein, RBAP48, RBBP4, Histone-binding protein RBBP4, Chromatin assembly factor 1 subunit C, Chromatin assembly factor I p48 subunit, Nucleosome-remodeling factor subunit RBAP48, Retinoblastoma-binding protein 4, Retinoblastoma-binding protein p48, CAF-1 subunit C, CAF-I 48 kDa subunit, CAF-I p48, RBBP-4, KMT6, EZH2, Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6

1 Images
SDS-PAGE - Recombinant Human EZH2 + SUZ12 + RbAp48 protein (His tag N-Term, His tag N-Term + GST tag N-Term) (AB198127)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human EZH2 + SUZ12 + RbAp48 protein (His tag N-Term, His tag N-Term + GST tag N-Term) (AB198127)

10% SDS-PAGE analysis of ab198127 with Coomassie staining.

Lane 1 : 2.8μg ab198127 complex
Lane 2 : Protein marker

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

Q15022

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.49% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

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Complementary Products

Proteins & Peptides

AB132934

Recombinant Human KMT6 / EZH2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human KMT6 / EZH2 protein (GST tag N-Terminus) (AB132934)

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Proteins & Peptides

AB156723

Recombinant Human EED protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human EED protein (His tag N-Terminus) (AB156723)

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Proteins & Peptides

AB198157

Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein (His tag N-Terminus, DDDDK tag N-Terminus)

Functional Studies - Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein (AB198157)

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Primary Antibodies

AB79416

Anti-RBBP4 antibody [EPR3411] - ChIP Grade

RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-RBBP4 antibody [EPR3411] - ChIP Grade (AB79416)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Requires EED protein for methyltransferase activity.</p>" } } }
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Product details

Useful for Western blot analysis and to investigate protein-protein interactions in the EZH2 complex. Requires EED protein for methyltransferase activity.
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Sequence info

[{"sequence":"ADKEAAFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTRPEGKDFSIHRLVLGTHTSDEQNHLVIASVQLPNDDAQFDASHYDSEKGEFGGFGSVSGKIEIEIKINHEGEVNRARYMPQNPCIIATKTPSSDVLVFDYTKHPSKPDPSGECNPDLRLRGHQKEGYGLSWNPNLSGHLLSASDDHTICLWDISAVPKEGKVVDAKTIFTGHTAVVEDVSWHLLHESLFGSVADDQKLMIWDTRSNNTSKPSHSVDAHTAEVNCLSFNPYSEFILATGSADKTVALWDLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLNVWDLSKIGEEQSPEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQVWQMAENIYNDEDPEGSVDPEGQGS","proteinLength":"Full Length","predictedMolecularWeight":"48 kDa","actualMolecularWeight":null,"aminoAcidEnd":425,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q09028","tags":[{"tag":"His","terminus":"N-Terminus"}]},{"sequence":"MAPQKHGGGGGGGSGPSAGSGGGGFGGSAAVAAATASGGKSGGGSCGGGGSYSASSSSSAAAAAGAAVLPVKKPKMEHVQADHELFLQAFEKPTQIYRFLRTRNLIAPIFLHRTLTYMSHRNSRTNIKRKTFKVDDMLSKVEKMKGEQESHSLSAHLQLTFTGFFHKNDKPSPNSENEQNSVTLEVLLVKVCHKKRKDVSCPIRQVPTGKKQVPLNPDLNQTKPGNFPSLAVSSNEFEPSNSHMVKSYSLLFRVTRPGRREFNGMINGETNENIDVNEELPARRKRNREDGEKTFVAQMTVFDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFETFSQGPTLQFTLRWTGETNDKSTAPIAKPLATRNSESLHQENKPGSVKPTQTIAVKESLTTDLQTRKEKDTPNENRQKLRIFYQFLYNNNTRQQTEARDDLHCPWCTLNCRKLYSLLKHLKLCHSRFIFNYVYHPKGARIDVSINECYDGSYAGNPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEVEQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIEEFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKNLCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEITEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL","proteinLength":"Full Length","predictedMolecularWeight":"87 kDa","actualMolecularWeight":null,"aminoAcidEnd":739,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q15022","tags":[{"tag":"His","terminus":"N-Terminus"}]},{"sequence":"GQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHRKCNYSFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVLESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIP","proteinLength":"Full Length","predictedMolecularWeight":"114 kDa","actualMolecularWeight":null,"aminoAcidEnd":751,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q15910","tags":[{"tag":"His","terminus":"N-Terminus"},{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target EZH2 + SUZ12 + RbAp48 forms a core part of the Polycomb Repressive Complex 2 (PRC2). EZH2 also known as Enhancer of Zeste Homolog 2 is a histone methyltransferase enzyme with a mass of about 85-97 kDa depending on isoforms and post-translational modifications. SUZ12 (Suppressor of Zeste 12 protein homolog) and RbAp48 (Retinoblastoma Binding Protein 4) work with EZH2 to regulate gene expression. Together they contribute to the trimethylation of histone H3 on lysine 27 (H3K27me3) silencing target genes. The expression of this complex varies but is often found in stem cells cancer cells and tissues undergoing rapid division.
Biological function summary

EZH2 SUZ12 and RbAp48 are significant in regulating gene expression through chromatin modification as part of the PRC2 complex. The complex plays a role in maintaining stem cell pluripotency and proper differentiation by stably repressing the transcription of specific gene sets. SUZ12 and RbAp48 stabilize the structure of the PRC2 complex facilitating the gene silencing activity of EZH2. Their activity highlights developmental processes such as cellular differentiation and proliferation.

Pathways

EZH2 + SUZ12 + RbAp48 heavily influence the Wnt signaling and Notch signaling pathways. In the Wnt pathway EZH2 can repress antagonists of Wnt signals modulating cellular responses. In the Notch pathway PRC2 helps in the transcriptional repression required for cellular differentiation. Proteins such as Beta-catenin in the Wnt pathway and Notch receptors in the Notch pathway are often interlinked with the regulatory functions of this complex.

EZH2 aberrations are closely associated with cancers and developmental disorders. Overexpression or mutations in EZH2 are linked with tumorigenesis especially in prostate cancer and lymphomas. In developmental disorders mutations in SUZ12 or depletion of RbAp48 may cause syndromes characterized by intellectual disability and growth retardation. In cancers the functional connection of EZH2 with proteins like VHL (Von Hippel-Lindau tumor suppressor) can contribute to oncogenic processes by impacting pathways critical to cell cycle regulation and apoptosis suppression.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Polycomb group (PcG) protein. Component of the PRC2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene (PubMed : 15225548, PubMed : 15231737, PubMed : 15385962, PubMed : 16618801, PubMed : 17344414, PubMed : 18285464, PubMed : 28229514, PubMed : 29499137, PubMed : 31959557). The PRC2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (PubMed : 12351676, PubMed : 12435631, PubMed : 15099518, PubMed : 15225548, PubMed : 15385962, PubMed : 15684044, PubMed : 16431907, PubMed : 18086877, PubMed : 18285464). Genes repressed by the PRC2 complex include HOXC8, HOXA9, MYT1 and CDKN2A (PubMed : 15231737, PubMed : 16618801, PubMed : 17200670, PubMed : 31959557).

Sequence similarities

Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.

Post-translational modifications

Sumoylated, probably by PIAS2.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Polycomb group (PcG) protein. Component of the PRC2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene (PubMed : 15225548, PubMed : 15231737, PubMed : 15385962, PubMed : 16618801, PubMed : 17344414, PubMed : 18285464, PubMed : 28229514, PubMed : 29499137, PubMed : 31959557). The PRC2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (PubMed : 12351676, PubMed : 12435631, PubMed : 15099518, PubMed : 15225548, PubMed : 15385962, PubMed : 15684044, PubMed : 16431907, PubMed : 18086877, PubMed : 18285464). Genes repressed by the PRC2 complex include HOXC8, HOXA9, MYT1 and CDKN2A (PubMed : 15231737, PubMed : 16618801, PubMed : 17200670, PubMed : 31959557).
See full target information SUZ12

Additional targets

RBBP4,EZH2
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