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Recombinant Human EZH2 + SUZ12 + RbAp48 protein is a Human Full Length protein, in the 2 to 425 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB.

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Images

SDS-PAGE - Recombinant Human EZH2 + SUZ12 + RbAp48 protein (AB198127), expandable thumbnail

Key facts

Purity
>95% SDS-PAGE
Expression system
Baculovirus infected Sf9 cells
Tags
His tag N-Terminus GST tag N-Terminus
Applications
SDS-PAGE, WB
Biologically active
No

Sequence

This product is comprised of multiple sequences see

Amino acid sequence 1

A D K E A A F D D A V E E R V I N E E Y K I W K K N T P F L Y D L V M T H A L E W P S L T A Q W L P D V T R P E G K D F S I H R L V L G T H T S D E Q N H L V I A S V Q L P N D D A Q F D A S H Y D S E K G E F G G F G S V S G K I E I E I K I N H E G E V N R A R Y M P Q N P C I I A T K T P S S D V L V F D Y T K H P S K P D P S G E C N P D L R L R G H Q K E G Y G L S W N P N L S G H L L S A S D D H T I C L W D I S A V P K E G K V V D A K T I F T G H T A V V E D V S W H L L H E S L F G S V A D D Q K L M I W D T R S N N T S K P S H S V D A H T A E V N C L S F N P Y S E F I L A T G S A D K T V A L W D L R N L K L K L H S F E S H K D E I F Q V Q W S P H N E T I L A S S G T D R R L N V W D L S K I G E E Q S P E D A E D G P P E L L F I H G G H T A K I S D F S W N P N E P W V I C S V S E D N I M Q V W Q M A E N I Y N D E D P E G S V D P E G Q G S

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

Requires EED protein for methyltransferase activity.

Target data

Function

Polycomb group (PcG) protein. Component of the PRC2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene (PubMed:15225548, PubMed:15231737, PubMed:15385962, PubMed:16618801, PubMed:17344414, PubMed:18285464, PubMed:28229514, PubMed:29499137, PubMed:31959557). The PRC2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (PubMed:12351676, PubMed:12435631, PubMed:15099518, PubMed:15225548, PubMed:15385962, PubMed:15684044, PubMed:16431907, PubMed:18086877, PubMed:18285464). Genes repressed by the PRC2 complex include HOXC8, HOXA9, MYT1 and CDKN2A (PubMed:15231737, PubMed:16618801, PubMed:17200670, PubMed:31959557).

Additional Targets

RBBP4, SUZ12

Alternative names

Recommended products

Recombinant Human EZH2 + SUZ12 + RbAp48 protein is a Human Full Length protein, in the 2 to 425 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB.

Key facts

Purity
>95% SDS-PAGE
Expression system
Baculovirus infected Sf9 cells
Applications
SDS-PAGE, WB
Accession
Q15022-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.49% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

Sequence info

Amino acid sequence 1

A D K E A A F D D A V E E R V I N E E Y K I W K K N T P F L Y D L V M T H A L E W P S L T A Q W L P D V T R P E G K D F S I H R L V L G T H T S D E Q N H L V I A S V Q L P N D D A Q F D A S H Y D S E K G E F G G F G S V S G K I E I E I K I N H E G E V N R A R Y M P Q N P C I I A T K T P S S D V L V F D Y T K H P S K P D P S G E C N P D L R L R G H Q K E G Y G L S W N P N L S G H L L S A S D D H T I C L W D I S A V P K E G K V V D A K T I F T G H T A V V E D V S W H L L H E S L F G S V A D D Q K L M I W D T R S N N T S K P S H S V D A H T A E V N C L S F N P Y S E F I L A T G S A D K T V A L W D L R N L K L K L H S F E S H K D E I F Q V Q W S P H N E T I L A S S G T D R R L N V W D L S K I G E E Q S P E D A E D G P P E L L F I H G G H T A K I S D F S W N P N E P W V I C S V S E D N I M Q V W Q M A E N I Y N D E D P E G S V D P E G Q G S
Accession
Q09028
Protein length
Full Length
Predicted molecular weight
48 kDa
Amino acids
2 to 425
Nature
Recombinant
Tags
His tag N-Terminus

Amino acid sequence 2

M A P Q K H G G G G G G G S G P S A G S G G G G F G G S A A V A A A T A S G G K S G G G S C G G G G S Y S A S S S S S A A A A A G A A V L P V K K P K M E H V Q A D H E L F L Q A F E K P T Q I Y R F L R T R N L I A P I F L H R T L T Y M S H R N S R T N I K R K T F K V D D M L S K V E K M K G E Q E S H S L S A H L Q L T F T G F F H K N D K P S P N S E N E Q N S V T L E V L L V K V C H K K R K D V S C P I R Q V P T G K K Q V P L N P D L N Q T K P G N F P S L A V S S N E F E P S N S H M V K S Y S L L F R V T R P G R R E F N G M I N G E T N E N I D V N E E L P A R R K R N R E D G E K T F V A Q M T V F D K N R R L Q L L D G E Y E V A M Q E M E E C P I S K K R A T W E T I L D G K R L P P F E T F S Q G P T L Q F T L R W T G E T N D K S T A P I A K P L A T R N S E S L H Q E N K P G S V K P T Q T I A V K E S L T T D L Q T R K E K D T P N E N R Q K L R I F Y Q F L Y N N N T R Q Q T E A R D D L H C P W C T L N C R K L Y S L L K H L K L C H S R F I F N Y V Y H P K G A R I D V S I N E C Y D G S Y A G N P Q D I H R Q P G F A F S R N G P V K R T P I T H I L V C R P K R T K A S M S E F L E S E D G E V E Q Q R T Y S S G H N R L Y F H S D T C L P L R P Q E M E V D S E D E K D P E W L R E K T I T Q I E E F S D V N E G E K E V M K L W N L H V M K H G F I A D N Q M N H A C M L F V E N Y G Q K I I K K N L C R N F M L H L V S M H D F N L I S I M S I D K A V T K L R E M Q Q K L E K G E S A S P A N E E I T E E Q N G T A N G F S E I N S K E K A L E T D S V S G V S K Q S K K Q K L
Accession
Q15022
Protein length
Full Length
Predicted molecular weight
87 kDa
Amino acids
1 to 739
Nature
Recombinant
Tags
His tag N-Terminus

Amino acid sequence 3

G Q T G K K S E K G P V C W R K R V K S E Y M R L R Q L K R F R R A D E V K S M F S S N R Q K I L E R T E I L N Q E W K Q R R I Q P V H I L T S V S S L R G T R E C S V T S D L D F P T Q V I P L K T L N A V A S V P I M Y S W S P L Q Q N F M V E D E T V L H N I P Y M G D E V L D Q D G T F I E E L I K N Y D G K V H G D R E C G F I N D E I F V E L V N A L G Q Y N D D D D D D D G D D P E E R E E K Q K D L E D H R D D K E S R P P R K F P S D K I F E A I S S M F P D K G T A E E L K E K Y K E L T E Q Q L P G A L P P E C T P N I D G P N A K S V Q R E Q S L H S F H T L F C R R C F K Y D C F L H R K C N Y S F H A T P N T Y K R K N T E T A L D N K P C G P Q C Y Q H L E G A K E F A A A L T A E R I K T P P K R P G G R R R G R L P N N S S R P S T P T I N V L E S K D T D S D R E A G T E T G G E N N D K E E E E K K D E T S S S S E A N S R C Q T P I K M K P N I E P P E N V E W S G A E A S M F R V L I G T Y Y D N F C A I A R L I G T K T C R Q V Y E F R V K E S S I I A P A P A E D V D T P P R K K K R K H R L W A A H C R K I Q L K K D G S S N H V Y N Y Q P C D H P R Q P C D S S C P C V I A Q N F C E K F C Q C S S E C Q N R F P G C R C K A Q C N T K Q C P C Y L A V R E C D P D L C L T C G A A D H W D S K N V S C K N C S I Q R G S K K H L L L A P S D V A G W G I F I K D P V Q K N E F I S E Y C G E I I S Q D E A D R R G K V Y D K Y M C S F L F N L N N D F V V D A T R K G N K I R F A N H S V N P N C Y A K V M M V N G D H R I G I F A K R A I Q T G E E L F F D Y R Y S Q A D A L K Y V G I E R E M E I P
Accession
Q15910
Protein length
Full Length
Predicted molecular weight
114 kDa
Amino acids
2 to 751
Nature
Recombinant
Tags
GST tag N-Terminus, His tag N-Terminus

Specifications

Form
Liquid
Additional notes

Affinity purified.

General info

Function

Polycomb group (PcG) protein. Component of the PRC2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene (PubMed:15225548, PubMed:15231737, PubMed:15385962, PubMed:16618801, PubMed:17344414, PubMed:18285464, PubMed:28229514, PubMed:29499137, PubMed:31959557). The PRC2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (PubMed:12351676, PubMed:12435631, PubMed:15099518, PubMed:15225548, PubMed:15385962, PubMed:15684044, PubMed:16431907, PubMed:18086877, PubMed:18285464). Genes repressed by the PRC2 complex include HOXC8, HOXA9, MYT1 and CDKN2A (PubMed:15231737, PubMed:16618801, PubMed:17200670, PubMed:31959557).

Sequence similarities

Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.

Post-translational modifications

Sumoylated, probably by PIAS2.

Subcellular localisation
Nucleus

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle

Notes

Useful for Western blot analysis and to investigate protein-protein interactions in the EZH2 complex. Requires EED protein for methyltransferase activity.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The target EZH2 + SUZ12 + RbAp48 forms a core part of the Polycomb Repressive Complex 2 (PRC2). EZH2 also known as Enhancer of Zeste Homolog 2 is a histone methyltransferase enzyme with a mass of about 85-97 kDa depending on isoforms and post-translational modifications. SUZ12 (Suppressor of Zeste 12 protein homolog) and RbAp48 (Retinoblastoma Binding Protein 4) work with EZH2 to regulate gene expression. Together they contribute to the trimethylation of histone H3 on lysine 27 (H3K27me3) silencing target genes. The expression of this complex varies but is often found in stem cells cancer cells and tissues undergoing rapid division.

Biological function summary

EZH2 SUZ12 and RbAp48 are significant in regulating gene expression through chromatin modification as part of the PRC2 complex. The complex plays a role in maintaining stem cell pluripotency and proper differentiation by stably repressing the transcription of specific gene sets. SUZ12 and RbAp48 stabilize the structure of the PRC2 complex facilitating the gene silencing activity of EZH2. Their activity highlights developmental processes such as cellular differentiation and proliferation.

Pathways

EZH2 + SUZ12 + RbAp48 heavily influence the Wnt signaling and Notch signaling pathways. In the Wnt pathway EZH2 can repress antagonists of Wnt signals modulating cellular responses. In the Notch pathway PRC2 helps in the transcriptional repression required for cellular differentiation. Proteins such as Beta-catenin in the Wnt pathway and Notch receptors in the Notch pathway are often interlinked with the regulatory functions of this complex.

Associated diseases and disorders

EZH2 aberrations are closely associated with cancers and developmental disorders. Overexpression or mutations in EZH2 are linked with tumorigenesis especially in prostate cancer and lymphomas. In developmental disorders mutations in SUZ12 or depletion of RbAp48 may cause syndromes characterized by intellectual disability and growth retardation. In cancers the functional connection of EZH2 with proteins like VHL (Von Hippel-Lindau tumor suppressor) can contribute to oncogenic processes by impacting pathways critical to cell cycle regulation and apoptosis suppression.

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1 product image

  • SDS-PAGE - Recombinant Human EZH2 + SUZ12 + RbAp48 protein (ab198127), expandable thumbnail

    SDS-PAGE - Recombinant Human EZH2 + SUZ12 + RbAp48 protein (ab198127)

    10% SDS-PAGE analysis of ab198127 with Coomassie staining.

    Lane 1: 2.8μg ab198127 complex
    Lane 2: Protein marker

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