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AB206788

Recombinant human FABP-1 protein (His tag N-Terminus)

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Recombinant human FABP-1 protein (His tag N-Terminus) is a Human Full Length protein, in the 30 to 430 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec.

View Alternative Names

KYAT4, GOT2, mAspAT, Fatty acid-binding protein, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, Transaminase A, FABP-1, FABPpm

3 Images
Western blot - Recombinant human FABP-1 protein (His tag N-Terminus) (AB206788)
  • WB

Unknown

Western blot - Recombinant human FABP-1 protein (His tag N-Terminus) (AB206788)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

All lanes:

Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (<a href='/en-us/products/primary-antibodies/aspartate-aminotransferase-fabp-1-antibody-epr12145-ab170950'>ab170950</a>) at 1/1000 dilution

All lanes:

Western blot - Recombinant human FABP-1 protein (His tag N-Terminus) (ab206788) at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 47 kDa

false

Exposure time: 180s

Western blot - Recombinant human FABP-1 protein (His tag N-Terminus) (AB206788)
  • WB

Lab

Western blot - Recombinant human FABP-1 protein (His tag N-Terminus) (AB206788)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FABP-1 antibody [EPR12354(B)] (<a href='/en-us/products/primary-antibodies/fabp-1-antibody-epr12354b-ab171739'>ab171739</a>) at 1/1000 dilution

All lanes:

Western blot - Recombinant human FABP-1 protein (His tag N-Terminus) (ab206788) at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 47 kDa

false

Exposure time: 10s

SDS-PAGE - Recombinant human FABP-1 protein (His tag N-Terminus) (AB206788)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human FABP-1 protein (His tag N-Terminus) (AB206788)

15% SDS-PAGE analysis of ab206788 (3μg).

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity is > 60 units/mg, and is defined as the amount of enzyme that convert 1μmole of α-ketoglutarate to L-Glutamate per
minute at pH 8.0 at 25°C.

Accession

P00505

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as GOT2.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSSSWWTHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDDNGKPYVLPSVRKAEAQIAAKNLDKEYLPIGGLAEFCKASAELALGENSEVLKSGRFVTVQTISGTGALRIGASFLQRFFKFSRDVFLPKPTWGNHTPIFRDAGMQLQGYRYYDPKTCGFDFTGAVEDISKIPEQSVLLLHACAHNPTGVDPRPEQWKEIATVVKKRNLFAFFDMAYQGFASGDGDKDAWAVRHFIEQGINVCLCQSYAKNMGLYGERVGAFTMVCKDADEAKRVESQLKILIRPMYSNPPLNGARIAAAILNTPDLRKQWLQEVKVMADRIIGMRTQLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVERLIKEFSIYMTKDGRISVAGVTSSNVGYLAHAIHQVTK","proteinLength":"Full Length","predictedMolecularWeight":"47 kDa","actualMolecularWeight":null,"aminoAcidEnd":430,"aminoAcidStart":30,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00505","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fatty Acid Binding Protein 1 (FABP-1) also known as Liver-type fatty acid-binding protein (L-FABP) is a small protein with a molecular weight of approximately 14 kDa. FABP-1 is abundantly expressed in the liver and also found in the intestine and kidneys. It functions by binding long-chain fatty acids and other lipophilic substances facilitating their transport within cells. The protein aids intracellular lipid trafficking assisting in the storage and metabolism of fatty acids.
Biological function summary

FABP-1 assists cells in maintaining lipid homeostasis by transporting these fatty acids to different cellular compartments for oxidation signaling or storage. It does not form part of a complex but independently interacts with lipids to buffer their concentration within the cytoplasm. Its role in lipid transport is essential for maintaining metabolic balance and energy production.

Pathways

FABP-1 participates in key metabolic pathways specifically the lipid metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathway. These pathways influence energy homeostasis and lipid modulation within cells. FABP-1 interacts with other proteins such as peroxisome proliferator-activated receptor alpha (PPARα) which regulates the expression of genes involved in lipid metabolic processes.

FABP-1 shows connections to liver conditions and metabolic syndrome. Altered levels of FABP-1 can indicate liver injury and could be used as a biomarker for non-alcoholic fatty liver disease (NAFLD). FABP-1 also connects to apolipoprotein E (ApoE) a protein involved in lipid metabolism in relation to the development and progression of atherosclerosis impacting cardiovascular health.
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Specifications

Form

Liquid

Additional notes

ab206788 was purified using conventional chromatography techniques.

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General info

Function

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.

Sequence similarities

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.
See full target information GOT2
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