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AB307479

Recombinant Human Factor D/CFD Protein (His tag) (Active)

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Recombinant Human Factor D/CFD Protein (His tag) (Active) is a Human Full Length protein, in the 26 to 253 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for HPLC, Mass Spec, SDS-PAGE.

View Alternative Names

DF, PFD, CFD, Complement factor D, Adipsin, C3 convertase activator, Properdin factor D

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Mass Spectrometry - Recombinant Human Factor D/CFD Protein (His tag) (Active) (AB307479)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human Factor D/CFD Protein (His tag) (Active) (AB307479)

Mass determination by ESI-TOF. Predicted MW is 25913.33 Da (+/-10 Da by ESI-TOF). Observed MW is 25914.02 Da.

Biological Activity - Recombinant Human Factor D/CFD Protein (His tag) (Active) (AB307479)
  • Biological Activity

Supplier Data

Biological Activity - Recombinant Human Factor D/CFD Protein (His tag) (Active) (AB307479)

Fully active determined by its ability to cleave colorimetric substrate N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl) in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is ≥ 149 pmol/min/ug.

SDS-PAGE - Recombinant Human Factor D/CFD Protein (His tag) (Active) (AB307479)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Factor D/CFD Protein (His tag) (Active) (AB307479)

SDS-PAGE analysis of ab307479

HPLC - Recombinant Human Factor D/CFD Protein (His tag) (Active) (AB307479)
  • HPLC

Supplier Data

HPLC - Recombinant Human Factor D/CFD Protein (His tag) (Active) (AB307479)

HPLC analysis of ab307479

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

Mass Spec, HPLC, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Fully active determined by its ability to cleave colorimetric substrate N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl) in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is ≥ 149 pmol/min/ug.

Accession

P00746

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"ILGGREAEAHARPYMASVQLNGAHLCGGVLVAEQWVLSAAHCLEDAADGKVQVLLGAHSLSQPEPSKRLYDVLRAVPHPDSQPDTIDHDLLLLQLSEKATLGPAVRPLPWQRVDRDVAPGTLCDVAGWGIVNHAGRRPDSLQHVLLPVLDRATCNRRTHHDGAITERLMCAESNRRDSCKGDSGGPLVCGGVLEGVVTSGSRVCGNRKKPGIYTRVASYAAWIDSVLA","proteinLength":"Full Length","predictedMolecularWeight":"25.91 kDa","actualMolecularWeight":"25.91 kDa","aminoAcidEnd":253,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P00746","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor D also known as complement factor D or CFD protein is a serine protease involved in the alternative pathway of the complement system. It plays a mechanical role by cleaving factor B when it is bound to C3b forming the active C3 convertase C3bBb. This enzyme is relatively small with a mass of approximately 28.5 kDa. It is mainly expressed in adipose tissue but it can also be found in the blood plasma.
Biological function summary

Factor D aids the immune system's ability to destroy pathogens. As part of the alternative complement pathway it acts independently and does not associate with larger protein complexes. Its activation of the alternative pathway leads to direct lysis of pathogens therefore contributing to innate immunity.

Pathways

Factor D participates specifically in the complement activation pathway particularly in the innate immune response. It works closely with other proteins in the complement system such as factor B and C3. This pathway enhances the opsonization and removal of microbes and damaged cells acting in parallel with the classical pathway of the complement system.

Abnormal function or regulation of factor D can relate to chronic inflammation and age-related macular degeneration. Disorders involving factor D may involve excess complement activation leading to tissue damage. The role of factor D in these diseases often connects it to other key complement components such as C3 and factor B which can also become problematic when dysregulated.
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Specifications

Form

Lyophilized

Additional notes

SDS-PAGE >= 95%

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General info

Function

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.

Sequence similarities

Belongs to the peptidase S1 family.

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Product protocols

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Target data

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
See full target information CFD
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