Recombinant human Factor H protein (His tag) is a Human Fragment protein, in the 860 to 1231 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
S I P L C V E K I P C S Q P P Q I E H G T I N S S R S S Q E S Y A H G T K L S Y T C E G G F R I S E E N E T T C Y M G K W S S P P Q C E G L P C K S P P E I S H G V V A H M S D S Y Q Y G E E V T Y K C F E G F G I D G P A I A K C L G E K W S H P P S C I K T D C L S L P S F E N A I P M G E K K D V Y K A G E Q V T Y T C A T Y Y K M D G A S N V T C I N S R W T G R P T C R D T S C V N P P T V Q N A Y I V S R Q M S K Y P S G E R V R Y Q C R S P Y E M F G D E E V M C L N G N W T E P P Q C K D S T G K C G P P P P I D N G D I T S F P L S V Y A P A S S V E Y Q C Q N L Y Q L E G N K R I T C R N G Q W S E P P K C L H P C V I S R E I M E N Y N I A L R W T A K Q K L Y S R T G E S V E F V C K R G Y R L S S R S H T L R T T C W D G K L E Y P T C A K R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces (PubMed:21285368, PubMed:25402769). Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop (PubMed:19503104, PubMed:26700768). As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b (PubMed:18252712, PubMed:23332154, PubMed:28671664). In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed (PubMed:20008295, PubMed:9558116). (Microbial infection) In the mosquito midgut, binds to the surface of parasite P.falciparum gametocytes and protects the parasite from alternative complement pathway-mediated elimination.
Alternative names
HF, HF1, HF2, CFH, Complement factor H, H factor 1
Recommended products
Recombinant human Factor H protein (His tag) is a Human Fragment protein, in the 860 to 1231 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its ability to bind biotinylated human DMP1 in a functional ELISA.
- Accession
- P08603-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- S I P L C V E K I P C S Q P P Q I E H G T I N S S R S S Q E S Y A H G T K L S Y T C E G G F R I S E E N E T T C Y M G K W S S P P Q C E G L P C K S P P E I S H G V V A H M S D S Y Q Y G E E V T Y K C F E G F G I D G P A I A K C L G E K W S H P P S C I K T D C L S L P S F E N A I P M G E K K D V Y K A G E Q V T Y T C A T Y Y K M D G A S N V T C I N S R W T G R P T C R D T S C V N P P T V Q N A Y I V S R Q M S K Y P S G E R V R Y Q C R S P Y E M F G D E E V M C L N G N W T E P P Q C K D S T G K C G P P P P I D N G D I T S F P L S V Y A P A S S V E Y Q C Q N L Y Q L E G N K R I T C R N G Q W S E P P K C L H P C V I S R E I M E N Y N I A L R W T A K Q K L Y S R T G E S V E F V C K R G Y R L S S R S H T L R T T C W D G K L E Y P T C A K R
- Accession
- P08603
- Protein length
- Fragment
- Predicted molecular weight
- 43 kDa
- Amino acids
- 860 to 1231
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces (PubMed:21285368, PubMed:25402769). Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop (PubMed:19503104, PubMed:26700768). As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b (PubMed:18252712, PubMed:23332154, PubMed:28671664). In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed (PubMed:20008295, PubMed:9558116).
- Post-translational modifications
Sulfated on tyrosine residues.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Factor H also known as complement factor H is an important regulatory protein in the complement system. It has a molecular mass of approximately 155 kDa. This protein is mainly expressed in the liver but it can also be found in low levels in other tissues. Factor H serves as a control element for complement activation particularly affecting the alternative pathway. It binds to C3b a central component of the complement system and accelerates the decay of C3 convertase as well as promotes the proteolytic inactivation of C3b by factor I.
- Biological function summary
Factor H limits the activity of the complement system to prevent damage to host tissues. The protein exists in the plasma in a soluble form. It functions by recognizing host cell surfaces via specific markers avoiding inappropriate activation. Factor H belongs to a group of proteins which include other regulators of complement activation. These proteins maintain the balance between effective immune defense and protection of host tissue from excessive immune responses.
- Pathways
Factor H is a part of the alternative complement pathway. This pathway is important for innate immune response involving proteins like factor P (properdin) which stabilizes C3 convertase. Factor H modulates these interactions to prevent unwarranted complement activity on host cells. Another related pathway is the classic complement pathway although factor H's involvement here is less direct since it primarily regulates the alternative pathway.
- Associated diseases and disorders
Factor H associations include atypical hemolytic uremic syndrome and age-related macular degeneration. Factor H deficiency or dysfunction can lead to uncontrolled complement activation resulting in kidney damage in atypical hemolytic uremic syndrome where it is also related to factor I. Additionally in age-related macular degeneration variants in the factor H gene are linked to increased susceptibility further highlighting the protein's importance in regulating immune responses.
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2 product images
Functional Studies - Recombinant human Factor H protein (His tag) (ab276309)
Measured by its ability to bind biotinylated human DMP1 in a functional ELISA.
SDS-PAGE - Recombinant human Factor H protein (His tag) (ab276309)
SDS-PAGE analysis of ab276309
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