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AB158403

Recombinant Human Factor VIII protein (GST tag N-Terminus)

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(1 Publication)

Recombinant Human Factor VIII protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 216 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

F8C, F8, Coagulation factor VIII, Antihemophilic factor, Procoagulant component, AHF

1 Images
SDS-PAGE - Recombinant Human Factor VIII protein (GST tag N-Terminus) (AB158403)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human Factor VIII protein (GST tag N-Terminus) (AB158403)

ab158403 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

P00451

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Assay Kits

AB300307

Human Factor IX/PTC ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

  • 5
  • 1
Primary Antibodies

AB126598

Anti-Antithrombin III/ATIII antibody [EP5372]

RabMAb|Recombinant

Western blot - Anti-Antithrombin III/ATIII antibody [EP5372] (AB126598)

  • 0
  • 0
Primary Antibodies

AB195028

Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Von Willebrand Factor antibody [EPSISR15] (AB195028)

  • 5
  • 1
Assay Kits

AB223864

Human Von Willebrand Factor ELISA Kit

Recombinant|SimpleStep

ELISA - Human Von Willebrand Factor ELISA Kit (AB223864)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MRIQDPGKVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGVTTQGVKSLLTSMYVKEFLISSSQDGHQWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY","proteinLength":"Full Length","predictedMolecularWeight":"51 kDa","actualMolecularWeight":null,"aminoAcidEnd":216,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P00451","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor VIII also known as anti-hemophilic factor is a glycoprotein with a molecular mass of approximately 250 kDa. This protein circulates in the plasma mainly in the liver where hepatocytes produce it. Factor VIII remains inactive while circulating in the blood and exists within the factor VIII/von Willebrand factor complex. Upon activation it plays a significant role in the blood coagulation process.
Biological function summary

Factor VIII greatly contributes to hemostasis by serving as a cofactor for factor IXa forming the intrinsic tenase complex. This activation results in the conversion of factor X to factor Xa which is important for the common pathway of the coagulation cascade. By participating in the clotting process factor VIII ensures proper wound healing and prevention of blood loss.

Pathways

Factor VIII activity is pivotal within the coagulation cascade particularly the intrinsic and common pathways. Activation of the intrinsic pathway begins through interactions with factor IXa leading to efficient activation of factor X. This process closely relates factor VIII to proteins like factor IX and factor X ensuring proper clot formation and stabilization. Factor VIII products and assays are available to study and characterize its critical role in these pathways.

Factor VIII is closely associated with hemophilia A a genetic bleeding disorder resulting from deficiency or dysfunction of this protein. Patients with hemophilia A exhibit prolonged bleeding times due to impaired coagulation. The disorder links directly to von Willebrand factor as it protects factor VIII from degradation in circulation stabilizing its presence in the blood. Anti-factor VIII antibodies are important tools for diagnosing and studying factor VIII-related disorders.
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Specifications

Form

Liquid

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General info

Function

Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.

Sequence similarities

Belongs to the multicopper oxidase family.

Post-translational modifications

Sulfation on Tyr-1699 is essential for binding vWF.. Proteolytically cleaved by cathepsin CTSG to produce a partially activated form.

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Product protocols

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Target data

Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.
See full target information F8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Mediterranean journal of hematology and infectious diseases 13:e2021041 PubMed34276910

2021

Hsa-miR-5581-3p and Hsa-miR-542-3p Target the Gene in Hemophilia A without Mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Feiying Meng
View all publications
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Product promise

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