Recombinant Human Factor X protein is a Human Full Length protein, in the 32 to 488 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
N N I L A R V T R A N S F L E E M K K G H L E R E C M E E T C S Y E E A R E V F E D S D K T N E F W N K Y K D G D Q C E T S P C Q N Q G K C K D G L G E Y T C T C L E G F E G K N C E L F T R K L C S L D N G D C D Q F C H E E Q N S V V C S C A R G Y T L A D N G K A C I P T G P Y P C G K Q T L E R R K R S V A Q A T S S S G E A P D S I T W K P Y D A A D L D P T E N P F D L L D F N Q T Q P E R G D N N L T R I V G G Q E C K D G E C P W Q A L L I N E E N E G F C G G T I L S E F Y I L T A A H C L Y Q A K R F K V R V G D R N T E Q E E G G E A V H E V E V V I K H N R F T K E T Y D F D I A V L R L K T P I T F R M N V A P A C L P E R D W A E S T L M T Q K T G I V S G F G R T H E K G R Q S T R L K M L E V P Y V D R N S C K L S S S F I I T Q N M F C A G Y D T K Q E D A C Q G D S G G P H V T R F K D T Y F V T G I V S W G E G C A R K G K Y G I Y T K V T A F L K W I D R S M K T R G L P K A K S H A P E V I T S S P L K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting (PubMed:22409427). Factor Xa activates pro-inflammatory signaling pathways in a protease-activated receptor (PAR)-dependent manner (PubMed:24041930, PubMed:30568593, PubMed:34831181, PubMed:18202198). Up-regulates expression of protease-activated receptors (PARs) F2R, F2RL1 and F2RL2 in dermal microvascular endothelial cells (PubMed:35738824). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, in cardiac fibroblasts and umbilical vein endothelial cells in PAR-1/F2R-dependent manner (PubMed:30568593, PubMed:34831181). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2, IL6, TNF-alpha/TNF, IL-1beta/IL1B, IL8/CXCL8 and IL18, in endothelial cells and atrial tissues (PubMed:24041930, PubMed:35738824, PubMed:9780208). Induces expression of adhesion molecules, such as ICAM1, VCAM1 and SELE, in endothelial cells and atrial tissues (PubMed:24041930, PubMed:35738824, PubMed:9780208). Increases expression of phosphorylated ERK1/2 in dermal microvascular endothelial cells and atrial tissues (PubMed:24041930, PubMed:35738824). Triggers activation of the transcription factor NF-kappa-B in dermal microvascular endothelial cells and atrial tissues (PubMed:24041930, PubMed:35738824). Activates pro-inflammatory and pro-fibrotic responses in dermal fibroblasts and enhances wound healing probably via PAR-2/F2RL1-dependent mechanism (PubMed:18202198). Activates barrier protective signaling responses in endothelial cells in PAR-2/F2RL1-dependent manner; the activity depends on the cleavage of PAR-2/F2RL1 by factor Xa (PubMed:22409427). Up-regulates expression of plasminogen activator inhibitor 1 (SERPINE1) in atrial tissues (PubMed:24041930).
Alternative names
Coagulation factor X, Stuart factor, Stuart-Prower factor, F10
Recommended products
Recombinant Human Factor X protein is a Human Full Length protein, in the 32 to 488 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P00742-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- N N I L A R V T R A N S F L E E M K K G H L E R E C M E E T C S Y E E A R E V F E D S D K T N E F W N K Y K D G D Q C E T S P C Q N Q G K C K D G L G E Y T C T C L E G F E G K N C E L F T R K L C S L D N G D C D Q F C H E E Q N S V V C S C A R G Y T L A D N G K A C I P T G P Y P C G K Q T L E R R K R S V A Q A T S S S G E A P D S I T W K P Y D A A D L D P T E N P F D L L D F N Q T Q P E R G D N N L T R I V G G Q E C K D G E C P W Q A L L I N E E N E G F C G G T I L S E F Y I L T A A H C L Y Q A K R F K V R V G D R N T E Q E E G G E A V H E V E V V I K H N R F T K E T Y D F D I A V L R L K T P I T F R M N V A P A C L P E R D W A E S T L M T Q K T G I V S G F G R T H E K G R Q S T R L K M L E V P Y V D R N S C K L S S S F I I T Q N M F C A G Y D T K Q E D A C Q G D S G G P H V T R F K D T Y F V T G I V S W G E G C A R K G K Y G I Y T K V T A F L K W I D R S M K T R G L P K A K S H A P E V I T S S P L K
- Accession
- P00742
- Protein length
- Full Length
- Amino acids
- 32 to 488
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting (PubMed:22409427). Factor Xa activates pro-inflammatory signaling pathways in a protease-activated receptor (PAR)-dependent manner (PubMed:24041930, PubMed:30568593, PubMed:34831181, PubMed:18202198). Up-regulates expression of protease-activated receptors (PARs) F2R, F2RL1 and F2RL2 in dermal microvascular endothelial cells (PubMed:35738824). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, in cardiac fibroblasts and umbilical vein endothelial cells in PAR-1/F2R-dependent manner (PubMed:30568593, PubMed:34831181). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2, IL6, TNF-alpha/TNF, IL-1beta/IL1B, IL8/CXCL8 and IL18, in endothelial cells and atrial tissues (PubMed:24041930, PubMed:35738824, PubMed:9780208). Induces expression of adhesion molecules, such as ICAM1, VCAM1 and SELE, in endothelial cells and atrial tissues (PubMed:24041930, PubMed:35738824, PubMed:9780208). Increases expression of phosphorylated ERK1/2 in dermal microvascular endothelial cells and atrial tissues (PubMed:24041930, PubMed:35738824). Triggers activation of the transcription factor NF-kappa-B in dermal microvascular endothelial cells and atrial tissues (PubMed:24041930, PubMed:35738824). Activates pro-inflammatory and pro-fibrotic responses in dermal fibroblasts and enhances wound healing probably via PAR-2/F2RL1-dependent mechanism (PubMed:18202198). Activates barrier protective signaling responses in endothelial cells in PAR-2/F2RL1-dependent manner; the activity depends on the cleavage of PAR-2/F2RL1 by factor Xa (PubMed:22409427). Up-regulates expression of plasminogen activator inhibitor 1 (SERPINE1) in atrial tissues (PubMed:24041930).
- Sequence similarities
Belongs to the peptidase S1 family.
- Post-translational modifications
The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Factor X also known as Stuart-Prower Factor is a critical component in the coagulation cascade. It has a molecular weight of approximately 59 kDa and is expressed mainly in the liver. This serine protease plays an important role in the conversion of prothrombin to thrombin an essential step in blood clot formation. Factor X circulates in the plasma as an inactive zymogen and becomes activated to its enzyme form factor Xa in response to specific physiological signals.
- Biological function summary
Factor X contributes significantly to the coagulation process by activating prothrombin into thrombin. It is not part of a larger complex but functions closely with other components of the coagulation cascade to maintain hemostasis. Factor X interacts with calcium ions and phospholipids on the surface of platelets enhancing its enzymatic activity. This activation ensures proper regulation of blood clotting preventing excessive bleeding or uncontrolled clot formation.
- Pathways
Factor X is central to both the intrinsic and extrinsic coagulation pathways. It interacts with proteins such as factor VIIa in the extrinsic pathway and factor IXa in the intrinsic pathway both pathways leading to the activation of factor Xa. In turn factor Xa is key in the common pathway which combines inputs from the intrinsic and extrinsic pathways to produce thrombin essential for fibrin clot formation.
- Associated diseases and disorders
Factor X is associated with bleeding disorders such as hemophilia and liver disease. Deficiencies or dysfunctions in factor X result in inadequate clot formation leading to prolonged bleeding episodes. Furthermore increased factor X activity as in cases of thrombophilia can raise the risk of abnormal blood clots. Factor X works alongside proteins like factor V with both deficiencies playing roles in hemophilia. Monitoring factor X activity is important in diagnosing and managing these coagulation-related conditions.
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1 product image
SDS-PAGE - Recombinant Human Factor X protein (ab158407)
ab158407 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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