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AB158407

Recombinant Human Factor X protein

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Recombinant Human Factor X protein is a Human Full Length protein, in the 32 to 488 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

Coagulation factor X, Stuart factor, Stuart-Prower factor, F10

1 Images
SDS-PAGE - Recombinant Human Factor X protein (AB158407)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Factor X protein (AB158407)

ab158407 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

P00742

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"NNILARVTRANSFLEEMKKGHLERECMEETCSYEEAREVFEDSDKTNEFWNKYKDGDQCETSPCQNQGKCKDGLGEYTCTCLEGFEGKNCELFTRKLCSLDNGDCDQFCHEEQNSVVCSCARGYTLADNGKACIPTGPYPCGKQTLERRKRSVAQATSSSGEAPDSITWKPYDAADLDPTENPFDLLDFNQTQPERGDNNLTRIVGGQECKDGECPWQALLINEENEGFCGGTILSEFYILTAAHCLYQAKRFKVRVGDRNTEQEEGGEAVHEVEVVIKHNRFTKETYDFDIAVLRLKTPITFRMNVAPACLPERDWAESTLMTQKTGIVSGFGRTHEKGRQSTRLKMLEVPYVDRNSCKLSSSFIITQNMFCAGYDTKQEDACQGDSGGPHVTRFKDTYFVTGIVSWGEGCARKGKYGIYTKVTAFLKWIDRSMKTRGLPKAKSHAPEVITSSPLK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":488,"aminoAcidStart":32,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P00742","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor X also known as Stuart-Prower Factor is a critical component in the coagulation cascade. It has a molecular weight of approximately 59 kDa and is expressed mainly in the liver. This serine protease plays an important role in the conversion of prothrombin to thrombin an essential step in blood clot formation. Factor X circulates in the plasma as an inactive zymogen and becomes activated to its enzyme form factor Xa in response to specific physiological signals.
Biological function summary

Factor X contributes significantly to the coagulation process by activating prothrombin into thrombin. It is not part of a larger complex but functions closely with other components of the coagulation cascade to maintain hemostasis. Factor X interacts with calcium ions and phospholipids on the surface of platelets enhancing its enzymatic activity. This activation ensures proper regulation of blood clotting preventing excessive bleeding or uncontrolled clot formation.

Pathways

Factor X is central to both the intrinsic and extrinsic coagulation pathways. It interacts with proteins such as factor VIIa in the extrinsic pathway and factor IXa in the intrinsic pathway both pathways leading to the activation of factor Xa. In turn factor Xa is key in the common pathway which combines inputs from the intrinsic and extrinsic pathways to produce thrombin essential for fibrin clot formation.

Factor X is associated with bleeding disorders such as hemophilia and liver disease. Deficiencies or dysfunctions in factor X result in inadequate clot formation leading to prolonged bleeding episodes. Furthermore increased factor X activity as in cases of thrombophilia can raise the risk of abnormal blood clots. Factor X works alongside proteins like factor V with both deficiencies playing roles in hemophilia. Monitoring factor X activity is important in diagnosing and managing these coagulation-related conditions.
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Specifications

Form

Liquid

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General info

Function

Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting (PubMed : 22409427). Factor Xa activates pro-inflammatory signaling pathways in a protease-activated receptor (PAR)-dependent manner (PubMed : 24041930, PubMed : 30568593, PubMed : 34831181, PubMed : 18202198). Up-regulates expression of protease-activated receptors (PARs) F2R, F2RL1 and F2RL2 in dermal microvascular endothelial cells (PubMed : 35738824). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, in cardiac fibroblasts and umbilical vein endothelial cells in PAR-1/F2R-dependent manner (PubMed : 30568593, PubMed : 34831181). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2, IL6, TNF-alpha/TNF, IL-1beta/IL1B, IL8/CXCL8 and IL18, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Induces expression of adhesion molecules, such as ICAM1, VCAM1 and SELE, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Increases expression of phosphorylated ERK1/2 in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Triggers activation of the transcription factor NF-kappa-B in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Activates pro-inflammatory and pro-fibrotic responses in dermal fibroblasts and enhances wound healing probably via PAR-2/F2RL1-dependent mechanism (PubMed : 18202198). Activates barrier protective signaling responses in endothelial cells in PAR-2/F2RL1-dependent manner; the activity depends on the cleavage of PAR-2/F2RL1 by factor Xa (PubMed : 22409427). Up-regulates expression of plasminogen activator inhibitor 1 (SERPINE1) in atrial tissues (PubMed : 24041930).

Sequence similarities

Belongs to the peptidase S1 family.

Post-translational modifications

The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.. Proteolytically cleaved and activated by cathepsin CTSG (PubMed:8920993). The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway) (By similarity).. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

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Product protocols

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Target data

Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting (PubMed : 22409427). Factor Xa activates pro-inflammatory signaling pathways in a protease-activated receptor (PAR)-dependent manner (PubMed : 24041930, PubMed : 30568593, PubMed : 34831181, PubMed : 18202198). Up-regulates expression of protease-activated receptors (PARs) F2R, F2RL1 and F2RL2 in dermal microvascular endothelial cells (PubMed : 35738824). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, in cardiac fibroblasts and umbilical vein endothelial cells in PAR-1/F2R-dependent manner (PubMed : 30568593, PubMed : 34831181). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2, IL6, TNF-alpha/TNF, IL-1beta/IL1B, IL8/CXCL8 and IL18, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Induces expression of adhesion molecules, such as ICAM1, VCAM1 and SELE, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Increases expression of phosphorylated ERK1/2 in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Triggers activation of the transcription factor NF-kappa-B in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Activates pro-inflammatory and pro-fibrotic responses in dermal fibroblasts and enhances wound healing probably via PAR-2/F2RL1-dependent mechanism (PubMed : 18202198). Activates barrier protective signaling responses in endothelial cells in PAR-2/F2RL1-dependent manner; the activity depends on the cleavage of PAR-2/F2RL1 by factor Xa (PubMed : 22409427). Up-regulates expression of plasminogen activator inhibitor 1 (SERPINE1) in atrial tissues (PubMed : 24041930).
See full target information F10
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