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AB140726

Recombinant Human FAM49B protein (His tag N-Terminus)

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Recombinant Human FAM49B protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 324 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

CYRI, FAM49B, BM-009, CYRIB, CYFIP-related Rac1 interactor B, L1

1 Images
SDS-PAGE - Recombinant Human FAM49B protein (His tag N-Terminus) (AB140726)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human FAM49B protein (His tag N-Terminus) (AB140726)

15% SDS-PAGE analysis of ab140726 (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9NUQ9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMGNLLKVLTCTDLEQGPNFFLDFENAQPTESEKEIYNQVNVVLKDAEGILEDLQSYRGAGHEIREAIQHPADEKLQEKAWGAVVPLVGKLKKFYEFSQRLEAALRGLLGALTSTPYSPTQHLEREQALAKQFAEILHFTLRFDELKMTNPAIQNDFSYYRRTLSRMRINNVPAEGENEVNNELANRMSLFYAEATPMLKTLSDATTKFVSENKNLPIENTTDCLSTMASVCRVMLETPEYRSRFTNEETVSFCLRVMVGVIILYDHVHPVGAFAKTSKIDMKGCIKVLKDQPPNSVEGLLNALRYTTKHLNDETTSKQIKSMLQ","proteinLength":"Full Length","predictedMolecularWeight":"39.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":324,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NUQ9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab140726 is purified using conventional chromatography techniques.

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General info

Function

Negatively regulates RAC1 signaling and RAC1-driven cytoskeletal remodeling (PubMed : 30250061, PubMed : 31285585). Regulates chemotaxis, cell migration and epithelial polarization by controlling the polarity, plasticity, duration and extent of protrusions. Limits Rac1 mediated activation of the Scar/WAVE complex, focuses protrusion signals and regulates pseudopod complexity by inhibiting Scar/WAVE-induced actin polymerization (PubMed : 30250061). Protects against Salmonella bacterial infection. Attenuates processes such as macropinocytosis, phagocytosis and cell migration and restrict sopE-mediated bacterial entry (PubMed : 31285585). Restricts also infection mediated by Mycobacterium tuberculosis and Listeria monocytogenes (By similarity). Involved in the regulation of mitochondrial dynamics and oxidative stress (PubMed : 29059164).

Sequence similarities

Belongs to the CYRI family.

Post-translational modifications

Ubiquitinated at Lys-74 upon Salmonella bacterial infection.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Negatively regulates RAC1 signaling and RAC1-driven cytoskeletal remodeling (PubMed : 30250061, PubMed : 31285585). Regulates chemotaxis, cell migration and epithelial polarization by controlling the polarity, plasticity, duration and extent of protrusions. Limits Rac1 mediated activation of the Scar/WAVE complex, focuses protrusion signals and regulates pseudopod complexity by inhibiting Scar/WAVE-induced actin polymerization (PubMed : 30250061). Protects against Salmonella bacterial infection. Attenuates processes such as macropinocytosis, phagocytosis and cell migration and restrict sopE-mediated bacterial entry (PubMed : 31285585). Restricts also infection mediated by Mycobacterium tuberculosis and Listeria monocytogenes (By similarity). Involved in the regulation of mitochondrial dynamics and oxidative stress (PubMed : 29059164).
See full target information CYRIB
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Complementary Products

Primary Antibodies

AB121299

Anti-FAM49B antibody

Immunocytochemistry/ Immunofluorescence - Anti-FAM49B antibody (AB121299)

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Cell Lines & Lysates

AB265906

Human FAM49B knockout HeLa cell line

Sanger Sequencing - Human FAM49B knockout HeLa cell line (AB265906)

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Cell Lines & Lysates

AB258868

Human FAM49B knockout HeLa cell lysate

Sanger Sequencing - Human FAM49B knockout HeLa cell lysate (AB258868)

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Product promise

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