Recombinant Human FANCA/FAA protein is a Human Full Length protein, in the 1 to 297 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M S D S W V P R S A S G Q D P G G R R R A W A E L L A G R V K R E K Y N P E R A Q K L K E S A V R L L R S H Q D L N A L L L E V E G P L C K K L S L S K V I D C D S S E A Y A N H S S S F I G S A L Q D Q A S R L G V P V G I L S A G M V A S S V G Q I C T A P A E T S H P V L L T V E Q R K K L S S L L E F A R Y L L A H S M F S R L S F C Q E L W K I Q S S L L L E A V W H L H V Q G I V S L Q E L L E S H P D M H A V G S W L F R N L C C L C E Q M E A S C Q H A D V A R A M L S D F V Q M F V L R G F Q K N S D L R R T V E P E K M P Q V A V D V L Q R M L I F A L D A L A A G V Q E E S S T H K I V R C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
Alternative names
FAA, FACA, FANCH, FANCA, Fanconi anemia group A protein, Protein FACA
Recommended products
Recombinant Human FANCA/FAA protein is a Human Full Length protein, in the 1 to 297 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- O15360-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M S D S W V P R S A S G Q D P G G R R R A W A E L L A G R V K R E K Y N P E R A Q K L K E S A V R L L R S H Q D L N A L L L E V E G P L C K K L S L S K V I D C D S S E A Y A N H S S S F I G S A L Q D Q A S R L G V P V G I L S A G M V A S S V G Q I C T A P A E T S H P V L L T V E Q R K K L S S L L E F A R Y L L A H S M F S R L S F C Q E L W K I Q S S L L L E A V W H L H V Q G I V S L Q E L L E S H P D M H A V G S W L F R N L C C L C E Q M E A S C Q H A D V A R A M L S D F V Q M F V L R G F Q K N S D L R R T V E P E K M P Q V A V D V L Q R M L I F A L D A L A A G V Q E E S S T H K I V R C
- Accession
- O15360
- Protein length
- Full Length
- Predicted molecular weight
- 58.41 kDa
- Amino acids
- 1 to 297
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
- Post-translational modifications
Phosphorylation is required for the formation of the nuclear complex. Not phosphorylated in cells derived from groups A, B, C, E, F, G, and H.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as FANCA.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FANCA protein also known as FAA protein is a critical component in the DNA repair mechanism. FANCA is approximately 162 kDa and shows widespread expression in the human body particularly in proliferating cells. This protein plays an essential role in maintaining genomic stability by participating in the repair of DNA interstrand crosslinks. FANCA's primary function involves its interaction with other proteins in the cell nucleus where DNA repair and replication processes occur.
- Biological function summary
FANCA works as a part of the Fanconi anemia (FA) core complex involving around 13 proteins. This complex plays an important role in the FA DNA repair pathway facilitating the repair of interstrand DNA crosslinks. FANCA acts by orchestrating the recruitment and activation of downstream repair proteins which ensures the high-fidelity maintenance of the genetic material during cell division. Dysfunction in the FA complex where FANCA operates can lead to chromosomal instability and increased mutation rates.
- Pathways
FANCA functions prominently in the Fanconi anemia pathway and is also involved in the homologous recombination repair pathway. In the FA pathway FANCA works closely with related proteins like FANCB and FANCC to initiate the DNA repair process. FANCA's interaction with these proteins allows it to execute its repair role effectively ensuring cellular genomic integrity. These pathways are critical for protecting cells from DNA damage during replication and shielding them from cancer development.
- Associated diseases and disorders
FANCA mutations are directly associated with Fanconi anemia a genetic disorder characterized by marrow failure cancer predisposition and various congenital abnormalities. Loss of FANCA function disrupts the repair pathway leading to cellular hypersensitivity to DNA-damaging agents. Additionally FANCA's impairment has been implicated in certain cancers like acute myeloid leukemia where its interaction with other FA proteins gets compromised leading to tumor progression.
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1 product image
SDS-PAGE - Recombinant Human FANCA/FAA protein (ab152374)
12.5% SDS-PAGE analysis of ab152374 stained with Coomassie Blue.
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Product protocols
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