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AB268550

Recombinant Human FANCL protein (Tagged)

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Recombinant Human FANCL protein (Tagged) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE.

View Alternative Names

PHF9, FANCL, E3 ubiquitin-protein ligase FANCL, Fanconi anemia group L protein, Fanconi anemia-associated polypeptide of 43 kDa, RING-type E3 ubiquitin transferase FANCL, FAAP43

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SDS-PAGE - Recombinant Human FANCL protein (Tagged) (AB268550)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human FANCL protein (Tagged) (AB268550)

SDS-PAGE analysis of ab268550.

Key facts

Purity

>85% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9NW38

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9NW38","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FANCL also known as Fanconi anemia group L protein participates in the Fanconi anemia (FA) pathway. It has a molecular mass of about 42 kDa. FANCL expresses in various tissues with notable expression in the bone marrow and blood-forming organs. The protein works mechanically by functioning as an E3 ubiquitin ligase essential for monoubiquitination of FANCD2 and FANCI which is an important step in the FA DNA repair pathway.
Biological function summary

This protein plays a role in DNA damage repair. FANCL is part of a multiprotein FA complex which is responsible for the repair of DNA interstrand crosslinks. The FA complex coordinates the activation and repair processes required to maintain genome stability. It ensures proper cell cycle progression while responding to DNA damage facilitating normal cellular function and development.

Pathways

FANCL is a component of the FA-BRCA pathway and has strong connections with DNA repair processes. This pathway includes interactions with proteins such as BRCA1 and BRCA2 which manage DNA repair to prevent tumorigenesis. FANCL also engages with elements of the homologous recombination repair pathway where it ensures proper DNA repair by managing the ubiquitination processes.

Defects in FANCL relate to Fanconi anemia a genetic disorder marked by bone marrow failure and cancer predisposition. The protein's association with BRCA2 also links to hereditary breast and ovarian cancers. These connections highlight FANCL's significance in maintaining genetic integrity and its potential impact on cancer development when impaired.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Ubiquitin ligase protein that mediates monoubiquitination of FANCD2 in the presence of UBE2T, a key step in the DNA damage pathway (PubMed : 12973351, PubMed : 16916645, PubMed : 17938197, PubMed : 19111657, PubMed : 24389026). Also mediates monoubiquitination of FANCI (PubMed : 19589784). May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.

Post-translational modifications

The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Ubiquitin ligase protein that mediates monoubiquitination of FANCD2 in the presence of UBE2T, a key step in the DNA damage pathway (PubMed : 12973351, PubMed : 16916645, PubMed : 17938197, PubMed : 19111657, PubMed : 24389026). Also mediates monoubiquitination of FANCI (PubMed : 19589784). May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.
See full target information FANCL
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