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AB316637

Recombinant Human FAP Protein Standard (His tag)

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Recombinant Human FAP Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.

View Alternative Names

Prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, Surface-expressed protease, FAPalpha, SIMP, Seprase, FAP

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Sandwich ELISA - Recombinant Human FAP Protein Standard (His tag) (AB316637)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human FAP Protein Standard (His tag) (AB316637)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human FAP Protein Standard (His tag) (AB316637)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human FAP Protein Standard (His tag) (AB316637)

SDS-PAGE analysis of ab316637 under reducing conditions for 2ug protein.

Key facts

Purity

>80% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

Q12884

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Primary Antibodies

AB207178

Anti-Fibroblast activation protein, alpha antibody [EPR20021]

KO Validated|BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (AB207178)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Assay Kits

AB256404

Human FAP ELISA Kit

Recombinant|SimpleStep

ELISA - Human FAP ELISA Kit (AB256404)

  • 0
  • 1
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

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Sequence info

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"86.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q12884","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAP also known as fibroblast activation protein alpha or seprase is a serine protease with a mass of approximately 97 kDa. It is largely expressed in embryonic tissues certain carcinomas and tissue remodeling sites. FAP acts mechanistically by exhibiting dipeptidyl peptidase and endopeptidase activities breaking down peptide chains. Cellular membrane-bound FAP localizes mainly on reactive stromal fibroblasts not normal tissues making it an interesting target for research.
Biological function summary

FAP plays a role in tissue remodeling and wound healing influencing the microenvironment of tumors and fibrotic tissues. It contributes to extracellular matrix degradation participating in the complex network of proteases. FAP is not a standalone unit but part of a larger complex that interacts with other proteases helping in cell migration and invasion. As a result its activity impacts processes like inflammation and carcinogenesis.

Pathways

FAP participates in processes related to tissue repair and cancer progression. Significant pathways include the degradation of the extracellular matrix and the PI3K/AKT signaling. It functions alongside proteins like DPP4 another dipeptidyl peptidase in these pathways. The matrix breakdown is central to cancer metastasis and wound healing enhancing our understanding of FAP's role in larger biological contexts.

FAP has links with cancer and fibrosis. Its expression correlates with aggressive tumors and poor prognosis in cancers such as breast and ovarian. In fibrotic diseases like pulmonary and liver fibrosis it contributes to excessive matrix deposition and tissue stiffening. FAP's involvement alongside collagenases in these disorders makes it a target of potential therapeutic interest.
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Specifications

Form

Liquid

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General info

Function

Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2 (PubMed : 14751930, PubMed : 16223769, PubMed : 16410248, PubMed : 16480718, PubMed : 17381073, PubMed : 18095711, PubMed : 21288888, PubMed : 24371721). Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vitronectin, tenascin, laminin, fibronectin, fibrin or casein (PubMed : 10347120, PubMed : 10455171, PubMed : 12376466, PubMed : 16223769, PubMed : 16651416, PubMed : 18095711, PubMed : 2172980, PubMed : 7923219, PubMed : 9065413). Also has dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro (PubMed : 10347120, PubMed : 10593948, PubMed : 16175601, PubMed : 16223769, PubMed : 16410248, PubMed : 16651416, PubMed : 17381073, PubMed : 21314817, PubMed : 24371721, PubMed : 24717288). Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB) (PubMed : 21314817). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.

Sequence similarities

Belongs to the peptidase S9B family.

Post-translational modifications

N-glycosylated.. The N-terminus may be blocked.

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Product protocols

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Target data

Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2 (PubMed : 14751930, PubMed : 16223769, PubMed : 16410248, PubMed : 16480718, PubMed : 17381073, PubMed : 18095711, PubMed : 21288888, PubMed : 24371721). Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vitronectin, tenascin, laminin, fibronectin, fibrin or casein (PubMed : 10347120, PubMed : 10455171, PubMed : 12376466, PubMed : 16223769, PubMed : 16651416, PubMed : 18095711, PubMed : 2172980, PubMed : 7923219, PubMed : 9065413). Also has dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro (PubMed : 10347120, PubMed : 10593948, PubMed : 16175601, PubMed : 16223769, PubMed : 16410248, PubMed : 16651416, PubMed : 17381073, PubMed : 21314817, PubMed : 24371721, PubMed : 24717288). Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB) (PubMed : 21314817). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.
See full target information FAP
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